The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit (DGKC Method) is a specialized diagnostic tool used in clinical laboratories to measure the activity of α-HBDH in human serum. This enzyme is crucial for assessing cardiac and liver health, making the kit an essential component in diagnostic procedures. The kit includes two reagents, R1 and R2, which are optimized to ensure accurate and reliable results. The assay is based on the DGKC method, a well-established technique in clinical chemistry.

1. The kit is designed with high precision and sensitivity, ensuring accurate measurement of α-HBDH activity. The reagents are formulated to minimize interference from other serum components, providing reliable results.
2. The composition of the kit includes R1 and R2 reagents. R1 contains Tris and α-ketobutyrate, while R2 contains β-NADH. These components work synergistically to facilitate the enzymatic reaction necessary for the assay.
3. The primary use of the kit is to measure α-HBDH activity in human serum, which is a key indicator of cardiac and liver function. This makes it an invaluable tool in clinical diagnostics, particularly in cases of myocardial infarction and liver disease.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a critical tool in clinical diagnostics. Its precise formulation and reliable performance make it a go-to choice for measuring α-HBDH activity, which is essential for assessing cardiac and liver health. The kit's design and composition ensure that it delivers accurate results, making it a trusted resource in medical laboratories.

The main features of the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit include its high sensitivity, precision, and reliability in measuring α-HBDH activity. The kit is designed to provide accurate results with minimal interference from other serum components.

1. High Sensitivity: The kit is capable of detecting low levels of α-HBDH activity, making it suitable for early diagnosis of cardiac and liver conditions.
2. Precision: The reagents are formulated to ensure consistent and reproducible results, reducing the likelihood of errors in measurement.
3. Reliability: The kit has been extensively tested and validated, ensuring that it performs reliably in clinical settings.
4. Ease of Use: The assay procedure is straightforward, requiring minimal training for laboratory personnel.
5. Compatibility: The kit is compatible with a wide range of clinical analyzers, making it versatile and easy to integrate into existing laboratory workflows.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its high sensitivity, precision, and reliability. These features make it an indispensable tool in clinical diagnostics, particularly for assessing cardiac and liver function. The kit's ease of use and compatibility with various analyzers further enhance its appeal, making it a preferred choice for medical laboratories.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings to measure α-HBDH activity in human serum. This measurement is crucial for diagnosing and monitoring cardiac and liver diseases.

1. Cardiac Diagnostics: The kit is used to assess myocardial infarction by measuring α-HBDH activity, which is elevated in cardiac tissue damage.
2. Liver Function Tests: α-HBDH activity is also a marker for liver function, making the kit useful in diagnosing liver diseases such as hepatitis and cirrhosis.
3. Monitoring Treatment: The kit can be used to monitor the effectiveness of treatments for cardiac and liver conditions by tracking changes in α-HBDH activity over time.
4. Research Applications: Beyond clinical diagnostics, the kit is also used in research settings to study the role of α-HBDH in various physiological and pathological processes.
5. Quality Control: The kit is employed in quality control processes to ensure the accuracy and reliability of clinical laboratory results.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, with applications ranging from cardiac and liver disease diagnosis to treatment monitoring and research. Its ability to provide accurate and reliable measurements of α-HBDH activity makes it an essential resource for medical professionals.

When purchasing the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use to ensure that the kit meets your laboratory's needs.

1. Product Specifications: Review the kit's specifications, including sensitivity, precision, and compatibility with your laboratory's analyzers.
2. Product Types: Consider the different types of kits available, such as those designed for specific analyzers or with additional features like extended shelf life.
3. Budget: Evaluate the cost of the kit in relation to your laboratory's budget, keeping in mind that higher-priced kits may offer additional features or better performance.
4. Intended Use: Ensure that the kit is suitable for your intended applications, whether for routine diagnostics, research, or quality control.

Purchasing the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can select a kit that best meets your laboratory's needs and ensures accurate and reliable results.

When using the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to prevent degradation of the reagents. Typically, this means refrigeration at 2-8°C.
2. Handling: Handle the reagents with care, avoiding contamination. Use clean pipettes and tips to prevent cross-contamination between samples.
3. Expiration Date: Always check the expiration date before use. Expired reagents may not provide accurate results.
4. Calibration: Regularly calibrate your analyzer to ensure that it is functioning correctly and providing accurate measurements.
5. Safety: Follow standard laboratory safety protocols, including wearing appropriate personal protective equipment (PPE) and disposing of waste materials properly.

Proper handling and storage of the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit are essential for obtaining accurate results. By following the recommended precautions, you can ensure the integrity of the reagents and maintain the reliability of your diagnostic tests. Always adhere to safety protocols to protect yourself and others in the laboratory.