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a-L-Fucosidase Assay Kit (cNPF Rate Method)
The a-L-Fucosidase Assay Kit is composed of two main reagents and calibration/control materials. Reagent 1 (R1) contains a dithiothreitol buffer at pH 5.0 with 100 mmol/L concentration, along with preservatives and stabilizers at 50 mmol/L. Reagent 2 (R2) includes the same buffer but is supplemented with 2-chloro-4-nitrophenyl-α-L-fucopyranoside (CNPF) at 20 mmol/L and preservatives/stabilizers. The kit also provides a single-level calibrator with 10% sucrose and α-L-fucosidase activity within 70±10 U/L, and two-level controls with 10% sucrose and α-L-fucosidase activity ranges of 30±10 U/L and 70±20 U/L.
Product English Name:
a-L-Fucosidase Assay Kit (cNPF Rate Method)
Alias:
Fucosidase Test Kit,Alpha-L-Fucosidase Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of α-L-fucosidase activity in human serum. It is widely used in clinical laboratories to assess enzyme activity, which can be indicative of certain metabolic disorders or liver diseases.
Product Introduction

The a-L-Fucosidase Assay Kit (cNPF Rate Method) is a specialized diagnostic tool used to measure the activity of α-L-fucosidase in human serum. This enzyme plays a crucial role in the breakdown of complex carbohydrates, and its activity levels can provide valuable insights into various metabolic conditions. The kit employs a colorimetric rate method, ensuring accurate and reliable results. It is an essential tool for clinical laboratories focusing on metabolic and liver-related diagnostics.

1. The kit features a highly sensitive and specific assay method, utilizing CNPF as a substrate to ensure precise measurement of α-L-fucosidase activity. The reaction is optimized for clinical settings, providing consistent and reproducible results.
2. The composition of the kit includes two reagents: R1 and R2. R1 contains a stabilizing buffer, while R2 includes the substrate CNPF. Additionally, the kit provides calibrators and controls to ensure the accuracy and reliability of the assay.
3. The primary use of this kit is to quantitatively determine α-L-fucosidase activity in human serum. This measurement is crucial for diagnosing and monitoring metabolic disorders, particularly those related to carbohydrate metabolism and liver function.

The a-L-Fucosidase Assay Kit, also known as the Fucosidase Test Kit, is a vital tool in clinical diagnostics. Its ability to accurately measure enzyme activity makes it indispensable for identifying metabolic disorders. The kit's robust design and reliable performance ensure that it meets the needs of modern clinical laboratories.

Main Features

The a-L-Fucosidase Assay Kit offers high sensitivity and specificity in measuring enzyme activity. Its optimized reagents and calibration materials ensure accurate and reproducible results, making it a reliable choice for clinical diagnostics.

1. The kit utilizes a colorimetric rate method, which provides a straightforward and reliable way to measure α-L-fucosidase activity. This method is highly sensitive and specific, ensuring accurate results.
2. The inclusion of calibrators and controls in the kit allows for precise calibration of the assay, ensuring that results are consistent and reliable across different batches and laboratories.
3. The reagents are carefully formulated to maintain stability and performance, even under varying storage conditions. This ensures that the kit remains effective over time.
4. The assay is designed for ease of use, with a simple protocol that can be easily integrated into routine laboratory workflows. This makes it accessible to a wide range of clinical settings.
5. The kit's ability to provide quantitative results makes it an essential tool for diagnosing and monitoring metabolic disorders, particularly those related to liver function and carbohydrate metabolism.

The a-L-Fucosidase Assay Kit stands out for its precision, reliability, and ease of use. Its ability to deliver consistent and accurate results makes it a trusted choice for clinical laboratories focused on metabolic diagnostics.

Clinical Applications

The a-L-Fucosidase Assay Kit is primarily used in clinical settings to diagnose and monitor metabolic disorders. Its ability to measure enzyme activity accurately makes it a valuable tool for identifying conditions related to carbohydrate metabolism and liver function.

1. The kit is widely used in clinical laboratories to assess α-L-fucosidase activity in patients suspected of having metabolic disorders. This measurement is crucial for accurate diagnosis and treatment planning.
2. In liver disease diagnostics, the kit helps in evaluating enzyme activity, which can be indicative of liver damage or dysfunction. This information is vital for monitoring disease progression and treatment efficacy.
3. The assay is also used in research settings to study the role of α-L-fucosidase in various metabolic pathways. This research can lead to a better understanding of metabolic disorders and the development of new treatments.
4. The kit's ability to provide quantitative results makes it useful for monitoring patients undergoing treatment for metabolic disorders. Regular testing can help track improvements or deteriorations in enzyme activity.
5. In addition to clinical and research applications, the kit is also used in quality control processes within diagnostic laboratories. Its reliable performance ensures that laboratory results are accurate and consistent.

The a-L-Fucosidase Assay Kit is a versatile tool with wide-ranging applications in clinical diagnostics and research. Its ability to provide accurate and reliable measurements of enzyme activity makes it indispensable for diagnosing and monitoring metabolic disorders.

Purchasing Guide

When purchasing the a-L-Fucosidase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product specifications: Ensure that the kit meets your laboratory's requirements in terms of sensitivity, specificity, and stability. Check the composition of the reagents and the availability of calibrators and controls.
2. Product types: The kit is available in different formats, including single-use and bulk options. Choose the format that best suits your laboratory's workflow and testing volume.
3. Budget: Consider the cost of the kit, including any additional expenses such as shipping and storage. Compare prices from different suppliers to find the best deal.
4. Intended use: Determine the specific applications for which you will be using the kit. Ensure that it is suitable for your intended use, whether for clinical diagnostics, research, or quality control.

Purchasing the a-L-Fucosidase Assay Kit requires careful consideration of various factors. By evaluating product specifications, types, budget, and intended use, you can select the kit that best meets your laboratory's needs.

 Precautions

When using the a-L-Fucosidase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Storage: Store the kit at the recommended temperature to maintain the stability and performance of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Use proper laboratory techniques when handling the reagents. Avoid contamination by using clean pipettes and tips for each sample.
3. Calibration: Regularly calibrate the assay using the provided calibrators to ensure accurate and consistent results. Follow the manufacturer's instructions for calibration procedures.
4. Quality Control: Run quality control samples with each batch of tests to monitor the performance of the assay. This helps identify any potential issues with the reagents or equipment.
5. Disposal: Dispose of used reagents and samples according to local regulations and laboratory protocols. Ensure that all waste is handled safely to prevent contamination.

Proper handling and storage of the a-L-Fucosidase Assay Kit are essential for obtaining accurate and reliable results. By following the recommended precautions, you can ensure the integrity of the reagents and maintain the performance of the assay.

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