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8-Plex Cytokine Detection Kit (Multiplex Microsphere Flow Cytometric Immunofluorescence Assay)
The kit consists of two main components: the detection component and the optional calibration component. The detection component includes capture microsphere antibodies, detection antibodies, SA-PE, assay buffer, and wash buffer (10×). The optional calibration component includes calibrators, matrix B, and assay buffer. The capture microsphere antibodies target IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17, IFN-γ, and TNF-α. The detection antibodies are biotin-labeled and target the same cytokines. SA-PE is streptavidin conjugated with phycoerythrin. The assay buffer contains BSA in phosphate buffer, and the wash buffer contains Tween-20 in phosphate buffer.
Product English Name:
8-Plex Cytokine Detection Kit (Multiplex Microsphere Flow Cytometric Immunofluorescence Assay)
Alias:
Cytokine Assay Kit,Multiplex Cytokine Detection Kit
Intended Use:
This kit is designed for the quantitative detection of IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17, IFN-γ, and TNF-α in human plasma. It is used in research and clinical settings to measure cytokine levels, which are critical for understanding immune responses and inflammatory conditions.
Product Introduction

The 8-Plex Cytokine Detection Kit is a state-of-the-art tool for simultaneously measuring eight key cytokines in human plasma. Utilizing a multiplex microsphere flow cytometric immunofluorescence assay, this kit offers high sensitivity and specificity. It is widely used in immunology research, clinical diagnostics, and drug development to assess immune system activity and monitor disease progression.

1. The kit features a multiplex design, allowing for the simultaneous detection of eight cytokines, which enhances efficiency and reduces sample volume requirements.
2. The capture microsphere antibodies are specifically designed to bind to their respective cytokines, ensuring high specificity. The detection antibodies are biotin-labeled, and SA-PE is used for signal amplification, providing a robust and sensitive detection system.
3. This kit is primarily used in research and clinical settings to quantify cytokine levels in human plasma, aiding in the study of immune responses, inflammatory diseases, and therapeutic interventions.

The 8-Plex Cytokine Detection Kit is a versatile and reliable tool for researchers and clinicians. Its ability to simultaneously measure multiple cytokines with high sensitivity makes it invaluable in understanding immune responses and disease mechanisms. The kit's design ensures ease of use and accurate results, making it a preferred choice in the field.

Main Features

The 8-Plex Cytokine Detection Kit offers high sensitivity, specificity, and multiplexing capability, allowing for the simultaneous detection of eight cytokines in a single sample.

1. The kit utilizes a multiplex microsphere flow cytometric immunofluorescence assay, which enables the detection of multiple cytokines in a single run, saving time and resources.
2. The capture microsphere antibodies are highly specific, ensuring accurate binding to their respective cytokines, while the biotin-labeled detection antibodies and SA-PE provide a strong and reliable signal.
3. The assay buffer and wash buffer are optimized for performance, ensuring consistent and reproducible results across different experiments.
4. The optional calibration component includes calibrators and matrix B, which are essential for standardizing the assay and ensuring accurate quantification of cytokine levels.
5. The kit is designed for ease of use, with clear instructions and a straightforward protocol, making it accessible to both experienced researchers and newcomers to the field.

The 8-Plex Cytokine Detection Kit stands out for its multiplexing capability, high sensitivity, and ease of use. It is a powerful tool for researchers and clinicians, providing reliable and accurate data on cytokine levels, which are crucial for understanding immune responses and disease mechanisms.

Clinical Applications

The 8-Plex Cytokine Detection Kit is widely used in clinical research and diagnostics to measure cytokine levels in human plasma, aiding in the study of immune responses and inflammatory diseases.

1. In clinical research, the kit is used to study the role of cytokines in various diseases, including autoimmune disorders, infections, and cancer.
2. The kit is also used in drug development to monitor the effects of new therapies on cytokine levels, helping to assess their efficacy and safety.
3. In clinical diagnostics, the kit is used to measure cytokine levels in patients with inflammatory diseases, providing valuable information for diagnosis and treatment.
4. The kit is also used in epidemiological studies to investigate the relationship between cytokine levels and disease prevalence in different populations.
5. Additionally, the kit is used in basic research to study the mechanisms of immune responses and the role of cytokines in health and disease.

The 8-Plex Cytokine Detection Kit is a versatile tool with wide-ranging applications in clinical research, diagnostics, and drug development. Its ability to accurately measure cytokine levels makes it invaluable for understanding immune responses and disease mechanisms, as well as for developing new therapies.

Purchasing Guide

When purchasing the 8-Plex Cytokine Detection Kit, consider factors such as product specifications, types, budget, and intended use to ensure the best fit for your research or clinical needs.

1. Product specifications: Ensure the kit meets your requirements for sensitivity, specificity, and the number of cytokines it can detect simultaneously.
2. Product types: Choose between kits with or without the optional calibration component, depending on your need for standardization and accuracy.
3. Budget: Consider the cost of the kit, including any additional components or reagents that may be required for your experiments.
4. Intended use: Select a kit that is suitable for your specific application, whether it is for research, clinical diagnostics, or drug development.

Purchasing the 8-Plex Cytokine Detection Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that the kit you choose will meet your research or clinical needs and provide reliable and accurate results.

 Precautions

When using the 8-Plex Cytokine Detection Kit, it is important to follow the instructions carefully and take necessary precautions to ensure accurate and reliable results.

1. Always wear appropriate personal protective equipment (PPE) when handling the kit components, especially the reagents and buffers.
2. Ensure that all components are stored at the recommended temperatures to maintain their stability and performance.
3. Follow the protocol precisely, paying close attention to incubation times, washing steps, and reagent volumes to avoid errors.
4. Use only high-quality samples, such as fresh or properly stored plasma, to ensure accurate cytokine measurements.
5. Regularly calibrate your equipment, such as the flow cytometer, to ensure consistent and reliable results.

Using the 8-Plex Cytokine Detection Kit requires careful attention to detail and adherence to safety protocols. By following the instructions and taking necessary precautions, you can ensure that your experiments yield accurate and reliable results, contributing to meaningful research and clinical outcomes.

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