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7-Plex Cytokine Detection Kit (Multiplex Microsphere Flow Cytometry Immunofluorescence Luminescence Method)
The kit consists of two main components: the detection component and the optional calibration component. The detection component includes capture microsphere antibodies, detection antibodies, SA-PE, assay buffer, and wash buffer (10×). The optional calibration component includes calibrators, Matrix B, and assay buffer. The capture microsphere antibodies target IL-4, IL-6, IL-10, IL-12p70, IL-17, IFN-γ, and TNF-α. Detection antibodies are biotin-labeled for the same cytokines. SA-PE is streptavidin conjugated with phycoerythrin. The assay buffer contains BSA in phosphate buffer, and the wash buffer contains Tween-20 in phosphate buffer.
Product English Name:
7-Plex Cytokine Detection Kit (Multiplex Microsphere Flow Cytometry Immunofluorescence Luminescence Method)
Alias:
Cytokine Assay Kit,Multiplex Cytokine Detection Kit
Intended Use:
This kit is designed for the quantitative detection of IL-4, IL-6, IL-10, IL-12p70, IL-17, IFN-γ, and TNF-α in human plasma. It is used in research and clinical settings to measure cytokine levels, which are critical for understanding immune responses, inflammation, and disease progression.
Product Introduction

The 7-Plex Cytokine Detection Kit is a state-of-the-art tool for simultaneously measuring seven key cytokines in human plasma. Utilizing multiplex microsphere flow cytometry immunofluorescence luminescence, this kit offers high sensitivity, specificity, and reproducibility. It is widely used in immunology research, clinical diagnostics, and drug development to assess immune system activity and monitor therapeutic responses.

1. The kit features a multiplex design, allowing for the simultaneous detection of seven cytokines, which significantly reduces sample volume and processing time. The use of microsphere-based flow cytometry ensures high sensitivity and specificity, making it ideal for low-abundance cytokine detection.
2. The kit's composition includes capture microsphere antibodies, detection antibodies, SA-PE, assay buffer, and wash buffer. The optional calibration component provides calibrators and Matrix B for accurate quantification. The reagents are carefully formulated to ensure stability and consistency across assays.
3. This kit is primarily used for research and clinical applications, including immune response monitoring, inflammation studies, and disease biomarker discovery. Its ability to quantitatively measure cytokine levels makes it a valuable tool for understanding immune system dynamics and evaluating therapeutic interventions.

The 7-Plex Cytokine Detection Kit is a versatile and reliable tool for researchers and clinicians. Its multiplex design and high sensitivity make it a go-to solution for cytokine analysis, while its comprehensive composition ensures accurate and reproducible results. Whether you're studying immune responses or developing new therapies, this kit provides the precision and efficiency you need.

Main Features

The 7-Plex Cytokine Detection Kit offers high sensitivity, multiplex capability, and reproducibility, making it an essential tool for cytokine analysis in research and clinical settings.

1. High Sensitivity: The kit's microsphere-based flow cytometry technology ensures the detection of low-abundance cytokines with exceptional sensitivity, even in complex biological samples.
2. Multiplex Capability: Simultaneously measure seven cytokines in a single sample, reducing the need for multiple assays and conserving valuable sample volume.
3. Reproducibility: The kit's standardized reagents and protocols ensure consistent results across experiments, enhancing data reliability.
4. Comprehensive Calibration: Optional calibrators and Matrix B provide accurate quantification, enabling precise measurement of cytokine concentrations.
5. User-Friendly Design: The kit includes detailed instructions and pre-formulated reagents, simplifying the assay process and reducing setup time.

The 7-Plex Cytokine Detection Kit stands out for its high sensitivity, multiplex capability, and user-friendly design. Whether you're a seasoned researcher or a clinician, this kit offers the tools you need to achieve accurate and reliable cytokine measurements, making it an indispensable asset in your laboratory.

Clinical Applications

This kit is widely used in clinical research and diagnostics to assess cytokine levels, monitor immune responses, and evaluate therapeutic interventions.

1. Immune Response Monitoring: The kit is used to measure cytokine levels in patients undergoing immunotherapy, helping clinicians assess treatment efficacy and adjust protocols accordingly.
2. Inflammation Studies: Researchers use the kit to study cytokine profiles in inflammatory diseases, providing insights into disease mechanisms and potential therapeutic targets.
3. Disease Biomarker Discovery: The kit aids in identifying cytokine biomarkers associated with specific diseases, facilitating early diagnosis and personalized treatment strategies.
4. Drug Development: Pharmaceutical companies use the kit to evaluate the impact of new drugs on cytokine levels, supporting the development of immunomodulatory therapies.
5. Clinical Diagnostics: The kit is employed in clinical labs to measure cytokine levels in patient samples, aiding in the diagnosis and management of immune-related disorders.

The 7-Plex Cytokine Detection Kit is a versatile tool with broad applications in clinical research and diagnostics. From monitoring immune responses to discovering disease biomarkers, this kit provides the precision and reliability needed to advance our understanding of immune system dynamics and improve patient care.

Purchasing Guide

This guide provides essential information for purchasing the 7-Plex Cytokine Detection Kit, including product specifications, types, budget considerations, and usage scenarios.

1. Product Specifications: The kit includes detection and calibration components, with detailed specifications provided in the product manual. Ensure compatibility with your laboratory equipment and workflow.
2. Product Types: Choose between standard and optional calibration kits based on your quantification needs. Consider additional reagents or accessories for enhanced functionality.
3. Budget Considerations: Evaluate the cost per sample and overall kit price to align with your research budget. Bulk purchasing or institutional discounts may be available.
4. Usage Scenarios: Assess your laboratory's capacity and workflow to determine the most suitable kit configuration. Ensure proper storage and handling to maintain reagent stability.

Purchasing the 7-Plex Cytokine Detection Kit requires careful consideration of product specifications, types, and budget. By understanding your laboratory's needs and workflow, you can select the right kit configuration to achieve accurate and reliable cytokine measurements, ensuring the success of your research or clinical applications.

 Precautions

Proper handling and storage of the 7-Plex Cytokine Detection Kit are essential to ensure accurate results and maintain reagent stability.

1. Storage Conditions: Store the kit at the recommended temperature to preserve reagent integrity. Avoid repeated freeze-thaw cycles for sensitive components.
2. Handling Precautions: Use sterile techniques and wear appropriate personal protective equipment (PPE) when handling reagents to prevent contamination.
3. Reagent Preparation: Follow the manufacturer's instructions for reconstituting and diluting reagents to ensure consistent performance.
4. Instrument Calibration: Regularly calibrate your flow cytometer or other detection instruments to maintain accuracy and reproducibility.
5. Waste Disposal: Dispose of used reagents and samples according to local regulations and laboratory safety protocols.

The 7-Plex Cytokine Detection Kit requires careful handling and storage to ensure optimal performance. By adhering to recommended protocols and safety measures, you can maintain reagent stability, prevent contamination, and achieve reliable results, making your research or clinical applications more effective and efficient.

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