The 5'-Nucleotidase Assay Kit is a specialized diagnostic tool used to measure the activity of 5'-nucleotidase in human serum. This enzyme is a key biomarker for liver and biliary tract disorders. The kit employs an enzymatic colorimetric method, ensuring high sensitivity and accuracy in detecting enzyme activity. It is an essential tool for clinical laboratories and research institutions focusing on liver health and disease diagnosis.

1. The kit features a highly sensitive enzymatic colorimetric method, ensuring precise measurement of 5'-nucleotidase activity. Its components are optimized for stability and performance, making it reliable for routine clinical use.
2. The kit consists of two reagents and a calibrator. Reagent 1 contains buffer, 4-AAP, PNP, peroxidase, and stabilizers, while Reagent 2 includes buffer, 5'-nucleotidase phosphate, and EHSPT. The calibrator is a lyophilized 5'-NT standard serum, providing a reference for accurate quantification.
3. This kit is primarily used for the quantitative determination of 5'-nucleotidase activity in human serum. It is a critical tool for diagnosing liver and biliary tract diseases, aiding in the assessment of liver function and the detection of hepatobiliary disorders.

The 5'-Nucleotidase Assay Kit, also known as the 5'-NT Test Kit, is a vital diagnostic tool for measuring enzyme activity in human serum. Its components are carefully formulated to ensure accuracy and reliability, making it indispensable in clinical settings for liver health assessment.

The 5'-Nucleotidase Assay Kit offers high sensitivity and accuracy in measuring enzyme activity. It is designed for ease of use in clinical laboratories, providing reliable results for liver function assessment.

1. The kit utilizes an enzymatic colorimetric method, ensuring precise and reproducible results. This method is highly sensitive, making it suitable for detecting low levels of 5'-nucleotidase activity.
2. The components of the kit, including Reagent 1 and Reagent 2, are optimized for stability and performance. The calibrator provides a reliable reference for accurate quantification.
3. The kit is designed for ease of use, with clear instructions and a straightforward protocol. It is suitable for routine use in clinical laboratories, reducing the risk of errors.
4. The kit is highly specific for 5'-nucleotidase, minimizing interference from other enzymes or substances in the serum. This specificity ensures accurate diagnosis of liver and biliary tract disorders.
5. The kit is compatible with a wide range of automated analyzers, making it versatile for use in different laboratory settings. Its adaptability enhances its utility in high-throughput environments.

The 5'-Nucleotidase Assay Kit stands out for its sensitivity, accuracy, and ease of use. Its optimized components and compatibility with automated analyzers make it a reliable choice for clinical laboratories focused on liver health.

The 5'-Nucleotidase Assay Kit is widely used in clinical settings for the diagnosis and monitoring of liver and biliary tract diseases. It provides critical insights into liver function and enzyme activity.

1. The kit is used in the diagnosis of hepatobiliary diseases, such as cholestasis and liver cirrhosis. It helps clinicians assess liver function and identify underlying conditions.
2. It is employed in monitoring the progression of liver diseases, providing valuable data for treatment planning and patient management.
3. The kit is used in research studies to investigate the role of 5'-nucleotidase in liver health and disease. It contributes to the development of new diagnostic and therapeutic approaches.
4. It is utilized in routine health check-ups to screen for liver disorders, especially in high-risk populations. Early detection of liver dysfunction can lead to timely intervention.
5. The kit is also used in toxicology studies to assess the impact of toxins and drugs on liver function. It provides a reliable measure of enzyme activity in response to external factors.

The 5'-Nucleotidase Assay Kit is a versatile tool in clinical practice, aiding in the diagnosis, monitoring, and research of liver and biliary tract diseases. Its applications extend to routine health screenings and toxicology studies, making it indispensable in liver health management.

When purchasing the 5'-Nucleotidase Assay Kit, consider factors such as product specifications, types, budget, and intended use. This guide provides essential tips for making an informed decision.

1. Product parameters: Ensure the kit meets your laboratory's requirements for sensitivity, accuracy, and compatibility with automated analyzers. Check the shelf life and storage conditions.
2. Product types: Choose between different kit formats, such as single-use or bulk packs, based on your laboratory's volume and frequency of use.
3. Budget: Compare prices from different suppliers, considering the cost per test and any additional expenses for shipping or storage.
4. Usage scenarios: Evaluate the kit's suitability for your specific applications, such as routine diagnostics, research, or toxicology studies. Ensure it aligns with your laboratory's workflow.

Purchasing the 5'-Nucleotidase Assay Kit requires careful consideration of product specifications, types, budget, and intended use. This guide helps you make an informed decision, ensuring the kit meets your laboratory's needs.

Proper handling and storage of the 5'-Nucleotidase Assay Kit are essential to ensure accurate results and prolong its shelf life. Follow these precautions to maintain the kit's integrity.

1. Store the kit at the recommended temperature, typically 2-8°C, to preserve the stability of its components. Avoid exposure to extreme temperatures or direct sunlight.
2. Handle the reagents with care, using appropriate protective equipment such as gloves and lab coats. Avoid contact with skin or eyes, and wash hands thoroughly after use.
3. Follow the manufacturer's instructions for reconstituting and using the calibrator. Ensure accurate pipetting and mixing to avoid errors in quantification.
4. Use the kit within its specified shelf life, and do not use expired reagents or calibrators. Regularly check the expiration dates and rotate stock as needed.
5. Dispose of used reagents and materials according to local regulations for biohazardous waste. Ensure proper disposal to prevent contamination or environmental harm.

The 5'-Nucleotidase Assay Kit requires careful handling and storage to ensure accurate results and longevity. By following these precautions, you can maintain the kit's integrity and reliability in clinical and research settings.