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5'-Nucleotidase (5'-NT) Assay Kit (Enzymatic Colorimetric Method)
The 5'-Nucleotidase (5'-NT) Assay Kit is composed of two main reagents: R1 and R2. R1 contains phosphate buffer, 4-aminoantipyrine, purine nucleoside phosphorylase, xanthine oxidase, and peroxidase. R2 includes phosphate buffer, 5'-inosinic acid sodium salt, and 3,5-dichloro-2-hydroxybenzenesulfonic acid sodium salt. These components work together to facilitate the enzymatic colorimetric detection of 5'-NT activity in human serum.
Product English Name:
5'-Nucleotidase (5'-NT) Assay Kit (Enzymatic Colorimetric Method)
Alias:
5'-NT Test Kit,Nucleotide Enzyme Assay Kit
Intended Use:
This kit is designed to measure the activity of 5'-nucleotidase in human serum, which is primarily used as an auxiliary diagnostic tool for hepatobiliary system diseases. It helps in identifying and monitoring conditions related to liver and bile duct health.
Product Introduction

The 5'-Nucleotidase (5'-NT) Assay Kit is a specialized diagnostic tool used to measure the activity of the enzyme 5'-nucleotidase in human serum. This enzyme is crucial for the metabolism of nucleotides and is often used as a biomarker for hepatobiliary system diseases. The kit employs an enzymatic colorimetric method, which provides a reliable and accurate measurement of enzyme activity. It is widely used in clinical laboratories for the diagnosis and monitoring of liver and bile duct disorders.

1. The 5'-NT Assay Kit is known for its high sensitivity and specificity in detecting 5'-nucleotidase activity. The enzymatic colorimetric method ensures accurate results, making it a reliable tool for clinical diagnostics.
2. The kit consists of two main reagents, R1 and R2, which contain a combination of enzymes and substrates necessary for the detection process. The precise formulation of these reagents ensures optimal performance and reproducibility.
3. The primary use of this kit is in the diagnosis and monitoring of hepatobiliary system diseases. By measuring 5'-NT activity, healthcare providers can gain valuable insights into liver and bile duct health, aiding in the early detection and management of related conditions.

The 5'-Nucleotidase (5'-NT) Assay Kit is a vital tool in the clinical laboratory, offering a reliable method for measuring 5'-NT activity. Its high sensitivity and specificity make it an essential component in the diagnosis of hepatobiliary system diseases. The kit's well-formulated reagents ensure accurate and reproducible results, making it a trusted choice for healthcare professionals.

Main Features

The 5'-NT Assay Kit is designed for the accurate measurement of 5'-nucleotidase activity in human serum. It features high sensitivity, specificity, and reproducibility, making it an essential tool for clinical diagnostics.

1. High Sensitivity: The kit is capable of detecting low levels of 5'-nucleotidase activity, ensuring accurate diagnosis even in early stages of disease.
2. Specificity: The enzymatic colorimetric method used in the kit is highly specific to 5'-nucleotidase, reducing the risk of false positives.
3. Reproducibility: The kit's reagents are formulated to provide consistent results across different batches and laboratories.
4. Ease of Use: The kit is designed for straightforward use in clinical laboratories, with clear instructions and minimal preparation required.
5. Comprehensive Analysis: The kit provides a complete analysis of 5'-nucleotidase activity, aiding in the comprehensive assessment of hepatobiliary health.

The 5'-NT Assay Kit stands out for its high sensitivity, specificity, and reproducibility. Its ease of use and comprehensive analysis capabilities make it an indispensable tool for clinical laboratories focused on hepatobiliary health.

Clinical Applications

The 5'-NT Assay Kit is primarily used in clinical settings for the diagnosis and monitoring of hepatobiliary system diseases. It provides valuable insights into liver and bile duct health.

1. Diagnosis of Liver Diseases: The kit is used to measure 5'-nucleotidase activity, which is elevated in various liver diseases, aiding in their diagnosis.
2. Monitoring Bile Duct Disorders: Elevated 5'-NT levels are also associated with bile duct disorders, making the kit useful for monitoring these conditions.
3. Early Detection: The kit's high sensitivity allows for the early detection of hepatobiliary system diseases, enabling timely intervention.
4. Research Applications: Beyond clinical diagnostics, the kit is also used in research settings to study the role of 5'-nucleotidase in various physiological and pathological processes.
5. Comprehensive Health Assessment: The kit provides a comprehensive assessment of hepatobiliary health, aiding in the overall management of patients with liver and bile duct disorders.

The 5'-NT Assay Kit is a versatile tool in clinical diagnostics, offering valuable insights into hepatobiliary health. Its applications range from disease diagnosis to research, making it an essential component in both clinical and research settings.

Purchasing Guide

When purchasing the 5'-NT Assay Kit, it is important to consider product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product Specifications: Ensure the kit meets the required sensitivity, specificity, and reproducibility standards for your laboratory's diagnostic needs.
2. Product Types: Consider the different types of 5'-NT Assay Kits available, such as those with varying reagent formulations or detection methods, to find the best fit for your application.
3. Budget: Evaluate the cost of the kit in relation to your laboratory's budget, considering both initial purchase and long-term usage costs.
4. Intended Use: Determine the specific clinical or research applications for which the kit will be used, ensuring it aligns with your laboratory's goals and requirements.

Purchasing the 5'-NT Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure the kit meets your laboratory's needs and provides reliable results for your diagnostic or research applications.

 Precautions

Proper handling and storage of the 5'-NT Assay Kit are essential to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to prevent degradation of the reagents, which could affect the accuracy of the results.
2. Handling Precautions: Follow the manufacturer's instructions for handling the reagents to avoid contamination or exposure to harmful substances.
3. Expiration Date: Always check the expiration date before use, as expired reagents may not provide accurate results.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure consistent and accurate measurements.
5. Disposal: Dispose of used reagents and materials according to local regulations to prevent environmental contamination.

Proper handling, storage, and disposal of the 5'-NT Assay Kit are crucial for maintaining its performance and ensuring accurate results. By following the recommended precautions, you can maximize the kit's effectiveness and reliability in your laboratory.

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