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5'-Nucleotidase (5'-NT) Assay Kit (Peroxidase Method)
The kit is composed of Reagent 1 (R1), Reagent 2 (R2), optional calibrators, and optional controls. Reagent 1 contains HEPES buffer, 4-aminoantipyrine, purine nucleoside phosphorylase (PNP), xanthine oxidase (XOD), and peroxidase (POD). Reagent 2 includes HEPES buffer, 5'-inosine monophosphate disodium salt (5'-IMP), and 2,4,6-triiodo-3-hydroxybenzoic acid. Calibrators and controls are based on bovine serum with specific target concentrations of 5'-nucleotidase.
Product English Name:
5'-Nucleotidase (5'-NT) Assay Kit (Peroxidase Method)
Alias:
5'-NT Kit,Nucleotidase Test Kit
Intended Use:
This kit is designed for the quantitative determination of 5'-nucleotidase activity in human serum in vitro. It is widely used in clinical laboratories to assess liver function and diagnose liver diseases.
Product Introduction

The 5'-Nucleotidase (5'-NT) Assay Kit (Peroxidase Method) is a diagnostic tool used to measure the activity of 5'-nucleotidase in human serum. This enzyme is crucial for nucleotide metabolism and is often used as a marker for liver function. The kit provides accurate and reliable results, making it a valuable resource for clinical diagnostics.

1. The kit features a high-sensitivity peroxidase method, ensuring precise detection of 5'-nucleotidase activity. It is designed for ease of use, with ready-to-use reagents that minimize preparation time.
2. The composition includes Reagent 1 and Reagent 2, which contain specific enzymes and buffers to facilitate the enzymatic reaction. Optional calibrators and controls are provided to ensure assay accuracy and reproducibility.
3. The primary use of this kit is in clinical laboratories for the diagnosis and monitoring of liver diseases. It is also used in research settings to study nucleotide metabolism and related biochemical pathways.

The 5'-Nucleotidase Assay Kit is a reliable and efficient tool for measuring 5'-nucleotidase activity. Its high sensitivity and ease of use make it a preferred choice for clinical diagnostics and research. The kit's comprehensive composition ensures accurate results, aiding in the diagnosis of liver-related conditions.

Main Features

The 5'-NT Assay Kit offers high sensitivity, accuracy, and ease of use, making it ideal for clinical diagnostics and research applications.

1. High Sensitivity: The peroxidase method ensures precise detection of 5'-nucleotidase activity, even at low concentrations.
2. Ready-to-Use Reagents: The kit includes pre-formulated reagents, reducing preparation time and minimizing errors.
3. Comprehensive Calibration: Optional calibrators and controls ensure assay accuracy and reproducibility across different samples.
4. Wide Application: Suitable for both clinical diagnostics and research, the kit is versatile and reliable.
5. User-Friendly: Designed for ease of use, the kit includes detailed instructions and requires minimal training.

The 5'-NT Assay Kit stands out for its high sensitivity, accuracy, and user-friendly design. It is a versatile tool that meets the needs of both clinical laboratories and research institutions, providing reliable results for 5'-nucleotidase activity measurement.

Clinical Applications

The 5'-NT Assay Kit is widely used in clinical settings for diagnosing liver diseases and monitoring liver function.

1. Liver Disease Diagnosis: The kit is used to measure 5'-nucleotidase activity, a key marker for liver function, aiding in the diagnosis of liver diseases.
2. Monitoring Treatment: It helps in monitoring the effectiveness of treatments for liver conditions by tracking changes in enzyme activity.
3. Research Applications: The kit is also used in biochemical research to study nucleotide metabolism and related pathways.
4. Clinical Trials: It is employed in clinical trials to assess the impact of new drugs on liver function.
5. Routine Check-ups: The kit is used in routine health check-ups to screen for liver abnormalities.

The 5'-NT Assay Kit is an essential tool in clinical diagnostics, particularly for liver disease diagnosis and monitoring. Its applications extend to research and clinical trials, making it a versatile and valuable resource in the medical field.

Purchasing Guide

This guide provides essential information for purchasing the 5'-NT Assay Kit, including product specifications, types, budget considerations, and usage scenarios.

1. Product Specifications: Ensure the kit meets your laboratory's requirements, including sensitivity, accuracy, and reagent composition.
2. Product Types: Choose between standard and customized kits based on your specific needs and application scope.
3. Budget Considerations: Evaluate the cost-effectiveness of the kit, considering the number of tests and additional components like calibrators and controls.
4. Usage Scenarios: Assess the kit's suitability for your intended applications, whether for clinical diagnostics, research, or routine check-ups.

Purchasing the 5'-NT Assay Kit requires careful consideration of product specifications, types, budget, and intended use. This guide aims to help you make an informed decision, ensuring the kit meets your laboratory's needs and provides reliable results.

 Precautions

Proper handling and storage of the 5'-NT Assay Kit are crucial to ensure accurate results and maintain reagent stability.

1. Storage Conditions: Store the kit at the recommended temperature to preserve reagent stability and prevent degradation.
2. Handling Precautions: Follow proper laboratory protocols to avoid contamination and ensure accurate results.
3. Reagent Preparation: Prepare reagents as instructed, ensuring proper mixing and avoiding exposure to light or heat.
4. Calibration and Controls: Use calibrators and controls as directed to validate assay performance and ensure reproducibility.
5. Disposal: Dispose of used reagents and materials according to local regulations and laboratory safety guidelines.

Adhering to proper storage, handling, and disposal protocols is essential for the effective use of the 5'-NT Assay Kit. These precautions help maintain reagent stability, ensure accurate results, and promote laboratory safety.

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