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5'-Nucleotidase Hydrolysis Enzyme Assay Kit (Enzyme Rate Method)
The kit consists of two main reagents: Reagent 1 contains a phosphate buffer (pH 7.60), 4-AAP, PNP, and peroxidase, while Reagent 2 includes a phosphate buffer (pH 7.60), XOD, 5'-nucleoside phosphate, and EHSPT. Additionally, there are two calibration solutions, each containing a specific amount of 5'-nucleotidase and stabilizers. Components from different batches are not interchangeable, and the calibration solutions are traceable to the enterprise working calibrator.
Product English Name:
5'-Nucleotidase Hydrolysis Enzyme Assay Kit (Enzyme Rate Method)
Alias:
5'-NT Assay Kit,Nucleotidase Activity Kit
Intended Use:
This kit is designed to measure the activity of 5'-nucleotidase (5'-NT) in human serum, primarily aiding in the diagnosis of hepatobiliary system diseases. It is a crucial tool for clinicians to assess liver and bile duct health.
Product Introduction

The 5'-Nucleotidase Hydrolysis Enzyme Assay Kit is a specialized diagnostic tool used to measure the activity of 5'-nucleotidase in human serum. This enzyme is a key biomarker for hepatobiliary system diseases, making the kit essential for clinical diagnostics. The kit employs an enzyme rate method, ensuring accurate and reliable results. It includes two main reagents and calibration solutions, all designed to work seamlessly together to provide precise measurements.

1. The kit features a highly sensitive enzyme rate method, ensuring accurate detection of 5'-nucleotidase activity. This method is known for its precision and reliability in clinical settings.
2. The composition of the kit includes two main reagents, each containing specific enzymes and buffers that facilitate the hydrolysis reaction. The calibration solutions are traceable to enterprise standards, ensuring consistency across batches.
3. The primary use of the kit is to assist in the diagnosis of hepatobiliary system diseases. By measuring 5'-nucleotidase activity, clinicians can gain valuable insights into liver and bile duct health.

The 5'-Nucleotidase Hydrolysis Enzyme Assay Kit, also known as the 5'-NT Assay Kit, is a vital tool in clinical diagnostics. Its precise enzyme rate method and carefully formulated reagents make it an indispensable resource for assessing hepatobiliary health. Whether you're a clinician or a researcher, this kit offers the accuracy and reliability you need for effective diagnosis.

Main Features

The main features of the 5'-Nucleotidase Hydrolysis Enzyme Assay Kit include its high sensitivity, precision, and reliability in measuring 5'-nucleotidase activity. The kit is designed for ease of use in clinical laboratories.

1. High Sensitivity: The kit employs an enzyme rate method that ensures highly sensitive detection of 5'-nucleotidase activity, making it suitable for clinical diagnostics.
2. Precision: The carefully formulated reagents and calibration solutions ensure precise measurements, reducing the likelihood of errors.
3. Reliability: The kit's components are traceable to enterprise standards, ensuring consistent performance across different batches.
4. Ease of Use: Designed for clinical laboratories, the kit is straightforward to use, with clear instructions and minimal preparation required.
5. Diagnostic Utility: The kit is specifically designed to aid in the diagnosis of hepatobiliary system diseases, providing clinicians with valuable diagnostic information.

The 5'-Nucleotidase Hydrolysis Enzyme Assay Kit stands out for its high sensitivity, precision, and reliability. Its ease of use and diagnostic utility make it an essential tool for clinicians and researchers alike, offering accurate and consistent results for hepatobiliary health assessment.

Clinical Applications

The 5'-Nucleotidase Hydrolysis Enzyme Assay Kit is primarily used in clinical settings to measure 5'-nucleotidase activity in human serum, aiding in the diagnosis of hepatobiliary system diseases.

1. Hepatobiliary Disease Diagnosis: The kit is widely used in clinical laboratories to assist in the diagnosis of liver and bile duct diseases, providing critical information on enzyme activity.
2. Research Applications: Researchers use the kit to study the role of 5'-nucleotidase in various diseases, contributing to a better understanding of hepatobiliary health.
3. Clinical Trials: The kit is employed in clinical trials to monitor the efficacy of new treatments for hepatobiliary diseases, ensuring accurate and reliable data collection.
4. Routine Health Checks: In some cases, the kit is used in routine health checks to assess liver function, particularly in patients with a history of liver disease.
5. Educational Purposes: The kit is also used in educational settings to teach students about enzyme activity measurement and its clinical significance.

The 5'-Nucleotidase Hydrolysis Enzyme Assay Kit is a versatile tool with wide-ranging applications in clinical diagnostics, research, and education. Its ability to accurately measure 5'-nucleotidase activity makes it invaluable for diagnosing and studying hepatobiliary system diseases.

Purchasing Guide

When purchasing the 5'-Nucleotidase Hydrolysis Enzyme Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure you select the right kit for your needs.

1. Product Specifications: Review the kit's specifications, including the enzyme rate method, reagent composition, and calibration solutions, to ensure it meets your requirements.
2. Product Types: Choose between different types of kits based on your specific needs, such as those designed for high-throughput laboratories or research purposes.
3. Budget: Consider your budget when selecting a kit, keeping in mind that higher-priced kits may offer additional features or higher precision.
4. Intended Use: Ensure the kit is suitable for your intended use, whether for clinical diagnostics, research, or educational purposes.

Purchasing the 5'-Nucleotidase Hydrolysis Enzyme Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can select the right kit to meet your diagnostic or research needs effectively.

 Precautions

When using the 5'-Nucleotidase Hydrolysis Enzyme Assay Kit, it is important to follow all instructions carefully to ensure accurate and reliable results. Proper handling and storage of the kit's components are also crucial.

1. Follow Instructions: Always adhere to the kit's instructions for use, including preparation, handling, and storage guidelines, to avoid errors.
2. Proper Storage: Store the kit's components at the recommended temperatures to maintain their stability and effectiveness.
3. Avoid Contamination: Use sterile techniques to prevent contamination of the reagents, which could affect the accuracy of the results.
4. Batch Specificity: Ensure that components from different batches are not mixed, as this could lead to inconsistent results.
5. Calibration: Regularly calibrate the kit using the provided calibration solutions to ensure accurate measurements.

Using the 5'-Nucleotidase Hydrolysis Enzyme Assay Kit requires careful attention to instructions, proper storage, and handling to ensure accurate results. By following these precautions, you can maximize the kit's effectiveness and reliability in clinical diagnostics.

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