Medical Instruments Encyclopedia
Home > Medical Instruments Encyclopedia
5'-Nucleotidase Assay Kit (Enzymatic Colorimetric Method)
The 5'-Nucleotidase Assay Kit is composed of two main reagents: R1 and R2. R1 contains glycine buffer, ALPS, purine nucleoside phosphorylase, xanthine oxidase, peroxidase, and stabilizers. R2 includes 5'-inosine monophosphate and 4-aminoantipyrine. Additionally, the kit comes with a calibrator, which is a liquid containing 5'-nucleotidase. This composition ensures accurate and reliable measurement of 5'-nucleotidase activity in human serum.
Product English Name:
5'-Nucleotidase Assay Kit (Enzymatic Colorimetric Method)
Alias:
5'-NT Assay Kit,Nucleotide Enzyme Test Kit
Intended Use:
This kit is specifically designed for the in vitro quantitative determination of 5'-nucleotidase (5'-NT) in human serum. It is widely used in clinical laboratories to assess liver function and diagnose liver diseases, as 5'-NT levels are often elevated in patients with hepatic disorders.
Product Introduction

The 5'-Nucleotidase Assay Kit (Enzymatic Colorimetric Method) is a diagnostic tool used to measure the activity of 5'-nucleotidase in human serum. This enzyme is crucial for the metabolism of nucleotides and is often used as a marker for liver function. The kit provides a reliable and accurate method for detecting 5'-NT levels, which can aid in the diagnosis and monitoring of liver diseases.

1. The kit is designed with a high level of sensitivity and specificity, ensuring accurate detection of 5'-nucleotidase activity. It utilizes a colorimetric method, which allows for easy and quick interpretation of results.
2. The composition of the kit includes two main reagents, R1 and R2, along with a calibrator. R1 contains a mixture of enzymes and stabilizers, while R2 includes the substrate and a chromogen. The calibrator ensures that the measurements are standardized and reliable.
3. The primary use of this kit is in clinical laboratories for the quantitative determination of 5'-nucleotidase in human serum. It is particularly useful in diagnosing liver diseases, as elevated levels of 5'-NT are often associated with hepatic disorders.

The 5'-Nucleotidase Assay Kit is an essential tool in clinical diagnostics, particularly for assessing liver function. Its high sensitivity and specificity make it a reliable choice for detecting 5'-NT levels. The kit's composition, including its reagents and calibrator, ensures accurate and standardized results. Overall, this kit is a valuable resource for healthcare professionals in diagnosing and monitoring liver diseases.

Main Features

The 5'-Nucleotidase Assay Kit offers a range of features that make it a reliable and efficient tool for measuring 5'-NT activity. Its enzymatic colorimetric method ensures accurate and quick results, while its composition guarantees stability and consistency.

1. The kit employs a colorimetric method, which allows for easy and quick interpretation of results. This method is highly sensitive and specific, ensuring accurate detection of 5'-nucleotidase activity.
2. The composition of the kit includes two main reagents, R1 and R2, along with a calibrator. R1 contains a mixture of enzymes and stabilizers, while R2 includes the substrate and a chromogen. This combination ensures reliable and consistent results.
3. The kit is designed for use in clinical laboratories, where it can be used to quantitatively determine 5'-nucleotidase levels in human serum. This is particularly useful in diagnosing liver diseases.
4. The kit is easy to use, with clear instructions provided for both preparation and interpretation of results. This makes it accessible to a wide range of healthcare professionals.
5. The kit is highly stable, with a long shelf life and consistent performance over time. This ensures that it can be used reliably in a variety of clinical settings.

The 5'-Nucleotidase Assay Kit is a versatile and reliable tool for measuring 5'-NT activity. Its colorimetric method, combined with its stable composition, ensures accurate and consistent results. The kit is easy to use and highly stable, making it a valuable resource for clinical laboratories. Overall, it is an essential tool for diagnosing and monitoring liver diseases.

Clinical Applications

The 5'-Nucleotidase Assay Kit is widely used in clinical settings for the diagnosis and monitoring of liver diseases. Its ability to accurately measure 5'-NT levels makes it a valuable tool for healthcare professionals.

1. The kit is primarily used in clinical laboratories to measure 5'-nucleotidase levels in human serum. This is particularly useful in diagnosing liver diseases, as elevated levels of 5'-NT are often associated with hepatic disorders.
2. The kit can also be used to monitor the progression of liver diseases over time. By regularly measuring 5'-NT levels, healthcare professionals can assess the effectiveness of treatments and make necessary adjustments.
3. In addition to liver diseases, the kit can be used to assess other conditions that may affect 5'-NT levels, such as certain types of cancer or metabolic disorders.
4. The kit is also useful in research settings, where it can be used to study the role of 5'-nucleotidase in various biological processes.
5. Overall, the 5'-Nucleotidase Assay Kit is a versatile tool that can be used in a variety of clinical and research settings to measure 5'-NT levels and assess liver function.

The 5'-Nucleotidase Assay Kit is a valuable tool in clinical diagnostics, particularly for assessing liver function. Its ability to accurately measure 5'-NT levels makes it essential for diagnosing and monitoring liver diseases. The kit is also useful in research settings, where it can be used to study the role of 5'-nucleotidase in various biological processes. Overall, it is a versatile and reliable tool for healthcare professionals.

Purchasing Guide

When purchasing the 5'-Nucleotidase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides an overview of key considerations to help you make an informed decision.

1. Product specifications: Ensure that the kit meets your laboratory's requirements in terms of sensitivity, specificity, and stability. Check the composition of the reagents and the shelf life of the kit.
2. Product types: There may be different versions of the kit available, such as those with different reagent volumes or additional components. Choose the one that best suits your needs.
3. Budget: Consider the cost of the kit, including any additional expenses such as shipping or storage. Ensure that the kit fits within your laboratory's budget.
4. Intended use: Determine the primary use of the kit, whether it is for clinical diagnostics, research, or both. This will help you choose the most appropriate version of the kit.

Purchasing the 5'-Nucleotidase Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you select the most appropriate kit for your laboratory's needs. Overall, this guide provides a comprehensive overview of key considerations to help you make an informed decision.

 Precautions

When using the 5'-Nucleotidase Assay Kit, it is important to follow certain precautions to ensure accurate and reliable results. This section provides an overview of key precautions to consider.

1. Always follow the manufacturer's instructions for preparation and use of the kit. This includes proper storage and handling of the reagents.
2. Ensure that all equipment used in the assay is clean and properly calibrated. Contamination or improper calibration can lead to inaccurate results.
3. Use the kit within its specified shelf life. Expired reagents may not provide accurate results.
4. Handle all reagents with care, as some components may be hazardous. Use appropriate personal protective equipment (PPE) when handling the kit.
5. Dispose of all waste materials according to local regulations. Some components of the kit may require special disposal procedures.

Using the 5'-Nucleotidase Assay Kit requires careful attention to certain precautions to ensure accurate and reliable results. By following the manufacturer's instructions, handling reagents with care, and properly disposing of waste materials, you can minimize the risk of errors and ensure the safety of laboratory personnel. Overall, these precautions are essential for the successful use of the kit in clinical and research settings.

Easy procurement

We have the latest medical device product database in China, which can quickly meet your procurement needs.

Submit your purchase list, and we will reply to your inquiry within half an hour at the earliest.

Quickly respond to your procurement needs:
Upload your purchase list
Support EXCEL/PDF/Word/compressed files