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Type IV Collagen Detection Kit (ELISA)
The kit includes essential components such as microplates coated with CIV monoclonal antibodies, a set of CIV calibrators (A-E) with concentrations ranging from 50 to 600 ng/ml, a conjugate antibody (rabbit anti-human CIV polyclonal antibody), enzyme conjugate (HRP-labeled goat anti-rabbit IgG), substrate A (containing urea peroxide), substrate B (containing TMB.2HCL), concentrated wash buffer (25×, containing PBS), and stop solution (2M H2SO4).
Product English Name:
Type IV Collagen Detection Kit (ELISA)
Alias:
CIV Detection Kit,Collagen IV Assay Kit
Intended Use:
This kit is designed for in vitro diagnostic use, specifically for the quantitative detection of Type IV Collagen (CIV) in human serum using an enzyme-linked immunosorbent assay (ELISA) method. It is suitable for clinical applications to assess CIV levels, which can be indicative of various health conditions.
Product Introduction

The Type IV Collagen Detection Kit (ELISA) is a sophisticated diagnostic tool designed for the accurate measurement of Type IV Collagen levels in human serum. This kit is essential for clinical laboratories and research institutions focusing on fibrosis, cancer, and other conditions where collagen metabolism is a key factor. The kit's components are meticulously designed to ensure high sensitivity and specificity, making it a reliable choice for both routine and specialized diagnostic procedures.

1. The kit features a highly sensitive ELISA method, ensuring precise detection of Type IV Collagen levels. The use of monoclonal antibodies provides high specificity, reducing cross-reactivity with other collagen types.
2. The kit includes a comprehensive set of components, such as pre-coated microplates, calibrators, and enzyme conjugates, all designed to work seamlessly together. The inclusion of a concentrated wash buffer and stop solution ensures efficient and accurate assay performance.
3. This kit is primarily used in clinical settings to measure Type IV Collagen levels, which are crucial for diagnosing and monitoring conditions such as liver fibrosis, kidney disease, and certain types of cancer. Its application extends to research studies focusing on extracellular matrix biology.

The Type IV Collagen Detection Kit (ELISA) stands out for its precision and reliability in measuring collagen levels. Its comprehensive design and ease of use make it a valuable tool in both clinical and research settings. The kit's ability to provide accurate results quickly and efficiently is a testament to its quality and the thoughtfulness behind its development.

Main Features

The main features of the Type IV Collagen Detection Kit include high sensitivity, specificity, and a comprehensive set of components designed for accurate and reliable results.

1. High Sensitivity: The kit's ELISA method ensures that even low concentrations of Type IV Collagen can be detected with precision, making it suitable for early diagnosis and monitoring.
2. Specificity: The use of monoclonal antibodies reduces cross-reactivity, ensuring that the results are specific to Type IV Collagen and not influenced by other collagen types.
3. Comprehensive Components: The kit includes all necessary components, from pre-coated microplates to calibrators and enzyme conjugates, ensuring a seamless assay process.
4. Efficient Wash and Stop Solutions: The concentrated wash buffer and stop solution are designed to optimize the assay, reducing background noise and enhancing result accuracy.
5. Clinical and Research Applications: The kit is versatile, suitable for both clinical diagnostics and research studies, providing valuable insights into collagen metabolism and related conditions.

The Type IV Collagen Detection Kit is a robust and reliable tool for measuring collagen levels. Its high sensitivity and specificity, combined with a comprehensive set of components, make it an indispensable resource for clinical and research applications. The kit's design ensures that users can achieve accurate results with minimal effort, making it a preferred choice in the field.

Clinical Applications

The Type IV Collagen Detection Kit is widely used in clinical settings for the diagnosis and monitoring of conditions related to collagen metabolism, such as liver fibrosis and kidney disease.

1. Liver Fibrosis Diagnosis: The kit is used to measure Type IV Collagen levels in serum, which are elevated in patients with liver fibrosis, aiding in early diagnosis and treatment planning.
2. Kidney Disease Monitoring: Elevated Type IV Collagen levels are also associated with kidney disease, and the kit is used to monitor disease progression and response to treatment.
3. Cancer Research: The kit is utilized in research studies to investigate the role of Type IV Collagen in cancer progression and metastasis, providing valuable insights into tumor biology.
4. Extracellular Matrix Studies: Researchers use the kit to study the extracellular matrix, particularly the role of Type IV Collagen in tissue repair and regeneration.
5. Clinical Trials: The kit is employed in clinical trials to assess the efficacy of new treatments targeting collagen metabolism, providing critical data for drug development.

The Type IV Collagen Detection Kit is a versatile tool with broad applications in both clinical diagnostics and research. Its ability to provide accurate and reliable measurements of Type IV Collagen levels makes it an essential resource for understanding and managing conditions related to collagen metabolism. The kit's applications extend from routine clinical use to cutting-edge research, highlighting its importance in the medical field.

Purchasing Guide

When purchasing the Type IV Collagen Detection Kit, consider factors such as product specifications, types, budget, and intended use to ensure the best fit for your needs.

1. Product Specifications: Review the kit's specifications, including sensitivity, specificity, and component details, to ensure it meets your diagnostic or research requirements.
2. Product Types: Consider the different types of kits available, such as those with varying calibrator ranges or additional components, to find the one that best suits your application.
3. Budget: Evaluate the cost of the kit in relation to your budget, keeping in mind that higher-quality kits may offer better performance and reliability.
4. Intended Use: Determine the primary use of the kit, whether for clinical diagnostics, research, or both, to ensure that the kit's features align with your goals.

Purchasing the Type IV Collagen Detection Kit requires careful consideration of various factors to ensure that it meets your specific needs. By evaluating product specifications, types, budget, and intended use, you can make an informed decision that will enhance your diagnostic or research capabilities. The right kit can significantly impact the accuracy and efficiency of your work, making it a worthwhile investment.

 Precautions

Proper handling and storage of the Type IV Collagen Detection Kit are essential to ensure accurate results and maintain the integrity of the components.

1. Storage Conditions: Store the kit at the recommended temperature to preserve the stability of the components, particularly the enzyme conjugates and substrates.
2. Handling: Use aseptic techniques when handling the kit components to prevent contamination, which could affect the assay results.
3. Expiration Date: Always check the expiration date before use, as expired components may not perform as expected, leading to inaccurate results.
4. Component Mixing: Follow the instructions carefully when mixing components to ensure proper reaction conditions and avoid errors in the assay.
5. Waste Disposal: Dispose of used components according to laboratory safety guidelines to prevent contamination and ensure a safe working environment.

The Type IV Collagen Detection Kit requires careful attention to storage, handling, and usage to ensure optimal performance. By adhering to the recommended precautions, users can maintain the integrity of the kit components and achieve accurate and reliable results. Proper handling not only enhances the quality of the assay but also ensures a safe and efficient laboratory environment.

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