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β-Hydroxybutyrate Assay Kit (Enzymatic Method)
The β-Hydroxybutyrate Assay Kit is composed of two main reagents: R1 and R2. R1 contains Tris buffer, oxalate, β-hydroxybutyrate dehydrogenase, and diaphorase. R2 includes INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride) and NAD+ (oxidized nicotinamide adenine dinucleotide). These components work together to facilitate the enzymatic reaction necessary for the quantitative determination of β-hydroxybutyrate in human serum.
Product English Name:
β-Hydroxybutyrate Assay Kit (Enzymatic Method)
Alias:
BHB Assay Kit,Ketone Body Test Kit
Intended Use:
This kit is specifically designed for the in vitro quantitative determination of β-hydroxybutyrate levels in human serum. It is commonly used in clinical settings to assess ketone body concentrations, which can be critical in diagnosing and managing conditions such as diabetic ketoacidosis, starvation, and other metabolic disorders.
Product Introduction

The β-Hydroxybutyrate Assay Kit (Enzymatic Method) is a highly reliable and accurate tool for measuring β-hydroxybutyrate levels in human serum. This kit is essential for clinical laboratories and research institutions focusing on metabolic studies. It provides a straightforward and efficient method for quantifying β-hydroxybutyrate, a key ketone body, which is crucial for understanding various metabolic conditions.

1. The kit is characterized by its high sensitivity and specificity, ensuring accurate detection of β-hydroxybutyrate levels even at low concentrations. The enzymatic method employed is both rapid and reliable, making it suitable for high-throughput clinical environments.
2. The composition of the kit includes two main reagents: R1 and R2. R1 contains Tris buffer, oxalate, β-hydroxybutyrate dehydrogenase, and diaphorase, while R2 includes INT and NAD+. These components work synergistically to facilitate the enzymatic reaction necessary for the quantitative determination of β-hydroxybutyrate.
3. The primary use of this kit is for the in vitro quantitative determination of β-hydroxybutyrate in human serum. It is particularly useful in clinical settings for diagnosing and managing conditions such as diabetic ketoacidosis, starvation, and other metabolic disorders.

The β-Hydroxybutyrate Assay Kit (Enzymatic Method) is a vital tool for clinical laboratories, offering a reliable and efficient way to measure β-hydroxybutyrate levels. Its high sensitivity and specificity make it indispensable for diagnosing and managing metabolic conditions. The kit's composition and enzymatic method ensure accurate and rapid results, making it a preferred choice in clinical settings.

Main Features

The β-Hydroxybutyrate Assay Kit offers high sensitivity, specificity, and reliability in measuring β-hydroxybutyrate levels. It is designed for ease of use in clinical laboratories, providing rapid and accurate results.

1. High Sensitivity: The kit can detect β-hydroxybutyrate at very low concentrations, making it suitable for early diagnosis of metabolic disorders.
2. Specificity: The enzymatic method ensures that the measurement is specific to β-hydroxybutyrate, reducing the risk of cross-reactivity with other substances.
3. Reliability: The kit provides consistent and reproducible results, which are crucial for clinical decision-making.
4. Ease of Use: The straightforward protocol allows for quick and efficient testing, even in high-throughput environments.
5. Rapid Results: The enzymatic reaction is fast, providing results in a short amount of time, which is essential in clinical settings.

The β-Hydroxybutyrate Assay Kit stands out for its high sensitivity, specificity, and reliability. Its ease of use and rapid results make it an invaluable tool in clinical laboratories, ensuring accurate and timely diagnosis of metabolic conditions.

Clinical Applications

The β-Hydroxybutyrate Assay Kit is widely used in clinical settings for the diagnosis and management of metabolic disorders such as diabetic ketoacidosis and starvation.

1. Diabetic Ketoacidosis: The kit is essential for diagnosing and monitoring diabetic ketoacidosis, a serious complication of diabetes.
2. Starvation: It helps in assessing ketone body levels in patients experiencing starvation or prolonged fasting.
3. Metabolic Disorders: The kit is used in the diagnosis and management of various metabolic disorders that affect ketone body production.
4. Research: It is also used in research settings to study the role of ketone bodies in metabolic processes.
5. Clinical Trials: The kit is employed in clinical trials to evaluate the efficacy of new treatments for metabolic disorders.

The β-Hydroxybutyrate Assay Kit is a crucial tool in clinical practice, particularly for diagnosing and managing diabetic ketoacidosis and other metabolic disorders. Its application extends to research and clinical trials, making it a versatile and essential component in the study of metabolic health.

Purchasing Guide

When purchasing the β-Hydroxybutyrate Assay Kit, consider product parameters, types, budget, and usage scenarios to ensure it meets your clinical or research needs.

1. Product Parameters: Evaluate the sensitivity, specificity, and reliability of the kit to ensure it meets your diagnostic requirements.
2. Product Types: Choose between different formats or sizes of the kit based on your laboratory's throughput and storage capacity.
3. Budget: Consider the cost-effectiveness of the kit, including the price per test and the overall value it provides.
4. Usage Scenarios: Assess the kit's suitability for your specific clinical or research applications, including the ease of integration into your existing workflows.

Purchasing the β-Hydroxybutyrate Assay Kit requires careful consideration of product parameters, types, budget, and usage scenarios. Ensuring the kit aligns with your clinical or research needs will guarantee optimal performance and value.

 Precautions

Proper handling, storage, and usage of the β-Hydroxybutyrate Assay Kit are essential to ensure accurate and reliable results.

1. Storage: Store the kit at the recommended temperature to maintain the stability and efficacy of the reagents.
2. Handling: Follow proper laboratory protocols to avoid contamination and ensure the integrity of the samples.
3. Usage: Adhere to the manufacturer's instructions for accurate and reproducible results.
4. Disposal: Dispose of used reagents and materials according to local regulations and laboratory safety guidelines.
5. Calibration: Regularly calibrate the equipment used with the kit to ensure consistent and accurate measurements.

To achieve the best results with the β-Hydroxybutyrate Assay Kit, it is crucial to follow proper storage, handling, and usage protocols. Regular calibration and adherence to safety guidelines will ensure the reliability and accuracy of the test results.

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