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Beta-Human Chorionic Gonadotropin (β-HCG) Assay Kit (Chemiluminescent Immunoassay)
The β-HCG Assay Kit is composed of several key components designed for accurate and reliable measurement of β-HCG levels in human serum. The kit includes a chemiluminescent coated plate with mouse anti-human β-HCG monoclonal antibodies, a set of calibrators with varying concentrations of β-HCG in bovine serum, a labeled antibody solution with horseradish peroxidase-conjugated mouse anti-human β-HCG monoclonal antibodies, and two chemiluminescent substrates (A and B) for signal generation. Additionally, a concentrated wash buffer is provided to ensure optimal assay performance.
Product English Name:
Beta-Human Chorionic Gonadotropin (β-HCG) Assay Kit (Chemiluminescent Immunoassay)
Alias:
β-HCG Test Kit,Chemiluminescent β-HCG Assay
Intended Use:
This kit is specifically designed for the quantitative determination of β-HCG levels in human serum. It is widely used in clinical laboratories to assess pregnancy, monitor trophoblastic diseases, and evaluate certain types of tumors. The assay provides a sensitive and accurate method for measuring β-HCG, which is crucial for diagnostic and monitoring purposes in various medical conditions.
Product Introduction

The β-HCG Assay Kit is a state-of-the-art diagnostic tool used for the quantitative measurement of β-HCG in human serum. Utilizing chemiluminescent immunoassay technology, this kit offers high sensitivity and specificity, making it an essential tool in clinical diagnostics. The kit is designed for ease of use, with all necessary components provided in a ready-to-use format, ensuring reliable and reproducible results.

1. The β-HCG Assay Kit features a chemiluminescent coated plate that is pre-coated with mouse anti-human β-HCG monoclonal antibodies. This design ensures high specificity and sensitivity in detecting β-HCG levels in serum samples.
2. The kit includes a set of calibrators with six different concentrations of β-HCG, ranging from 0 to 1000 mIU/mL. These calibrators are essential for establishing a standard curve, which is used to quantify β-HCG levels in patient samples accurately.
3. The labeled antibody solution contains horseradish peroxidase-conjugated mouse anti-human β-HCG monoclonal antibodies, which bind specifically to β-HCG in the sample. This binding is then detected using chemiluminescent substrates, providing a highly sensitive and accurate measurement of β-HCG levels.

The β-HCG Assay Kit, also known as the Chemiluminescent β-HCG Assay, is a reliable and efficient tool for measuring β-HCG levels in human serum. Its high sensitivity and specificity make it indispensable in clinical diagnostics, particularly in the assessment of pregnancy and certain medical conditions. The kit's comprehensive design ensures ease of use and accurate results, making it a valuable asset in any clinical laboratory.

Main Features

The β-HCG Assay Kit offers a range of features designed to ensure accurate and reliable measurement of β-HCG levels. These include high sensitivity, specificity, and ease of use, making it a preferred choice for clinical diagnostics.

1. High Sensitivity: The kit's chemiluminescent technology allows for the detection of very low levels of β-HCG, ensuring accurate results even in early pregnancy or low-concentration samples.
2. Specificity: The use of mouse anti-human β-HCG monoclonal antibodies ensures that the assay specifically targets β-HCG, minimizing cross-reactivity with other substances.
3. Ease of Use: The kit is designed for straightforward operation, with all components provided in a ready-to-use format, reducing the need for complex preparation steps.
4. Reproducibility: The inclusion of a set of calibrators with known concentrations ensures that the assay produces consistent and reproducible results across different runs.
5. Comprehensive Components: The kit includes all necessary reagents and materials, from the coated plate to the chemiluminescent substrates, ensuring a complete and efficient assay process.

The β-HCG Assay Kit stands out for its high sensitivity, specificity, and ease of use. These features make it an essential tool in clinical diagnostics, particularly for the accurate measurement of β-HCG levels in various medical conditions. The kit's comprehensive design ensures reliable and reproducible results, making it a valuable addition to any clinical laboratory.

Clinical Applications

The β-HCG Assay Kit is widely used in clinical settings for the quantitative measurement of β-HCG levels. Its applications include pregnancy testing, monitoring of trophoblastic diseases, and evaluation of certain tumors.

1. Pregnancy Testing: The kit is commonly used to confirm pregnancy by detecting the presence of β-HCG in serum, which is produced by the placenta shortly after implantation.
2. Monitoring Trophoblastic Diseases: The assay is used to monitor β-HCG levels in patients with trophoblastic diseases, such as hydatidiform mole or choriocarcinoma, where β-HCG levels are significantly elevated.
3. Tumor Evaluation: Certain tumors, particularly germ cell tumors, can produce β-HCG. The kit is used to measure β-HCG levels in these patients, aiding in diagnosis and monitoring treatment efficacy.
4. Assisted Reproductive Technology (ART): In ART, β-HCG levels are monitored to assess the success of embryo implantation and early pregnancy development.
5. Miscarriage and Ectopic Pregnancy: The kit is also used to monitor β-HCG levels in cases of suspected miscarriage or ectopic pregnancy, where abnormal β-HCG levels can indicate complications.

The β-HCG Assay Kit is a versatile tool in clinical diagnostics, with applications ranging from pregnancy testing to the monitoring of trophoblastic diseases and certain tumors. Its ability to provide accurate and reliable β-HCG measurements makes it an indispensable resource in various medical scenarios, ensuring effective diagnosis and treatment monitoring.

Purchasing Guide

When purchasing the β-HCG Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use to ensure the best fit for your clinical laboratory needs.

1. Product Specifications: Review the kit's specifications, including sensitivity, specificity, and the range of β-HCG concentrations it can detect, to ensure it meets your laboratory's requirements.
2. Product Types: Consider the different types of β-HCG assay kits available, such as those with varying numbers of tests or different formats (e.g., manual vs. automated), to choose the one that best suits your workflow.
3. Budget: Evaluate the cost of the kit, including any additional reagents or equipment that may be required, to ensure it fits within your laboratory's budget.
4. Intended Use: Determine the primary applications for the kit in your laboratory, such as pregnancy testing or tumor monitoring, to ensure it aligns with your diagnostic needs.

Purchasing the β-HCG Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By thoroughly evaluating these aspects, you can select the kit that best meets your clinical laboratory's needs, ensuring accurate and reliable β-HCG measurements for your diagnostic applications.

 Precautions

When using the β-HCG Assay Kit, it is important to follow specific precautions to ensure accurate results and maintain the integrity of the components.

1. Storage Conditions: Store the kit components at the recommended temperatures to maintain their stability and performance. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Handle all components with care, especially the chemiluminescent substrates, which are light-sensitive. Use appropriate protective equipment, such as gloves, to avoid contamination.
3. Calibration: Ensure that the calibrators are used according to the instructions, as they are batch-specific and critical for accurate quantification of β-HCG levels.
4. Sample Preparation: Follow the recommended procedures for serum sample collection and preparation to avoid hemolysis or other factors that could affect the assay results.
5. Disposal: Dispose of all used materials, including the coated plate and any remaining reagents, in accordance with local regulations for biohazardous waste.

Using the β-HCG Assay Kit requires adherence to specific precautions to ensure accurate and reliable results. Proper storage, handling, calibration, sample preparation, and disposal are essential to maintain the integrity of the kit components and achieve optimal assay performance. By following these guidelines, you can ensure the successful use of the kit in your clinical laboratory.

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