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25-Hydroxy Vitamin D Assay Kit (Chemiluminescence Method)
The kit primarily consists of magnetic bead-coated particles (R1), enzyme-labeled conjugates (R2), reaction buffer (R3), sample treatment solution (R4), optional calibrators, and optional controls. R1 contains superparamagnetic particles coated with 25-hydroxy vitamin D antigen and preservatives (ProClin 300). R2 includes 25-hydroxy vitamin D antibody-alkaline phosphatase (AP enzyme) conjugates with preservatives. R3 is a reaction buffer with dithiothreitol, stabilizers, and a pH of 5.0. R4 is a sample treatment solution containing sodium hydroxide and preservatives. Calibrators and controls are lyophilized products containing 25-hydroxy vitamin D antigen.
Product English Name:
25-Hydroxy Vitamin D Assay Kit (Chemiluminescence Method)
Alias:
Vitamin D Test Kit,25(OH)D Assay Kit
Intended Use:
This kit is designed for the quantitative measurement of 25-hydroxy vitamin D in human serum and/or plasma. It is clinically used for the auxiliary diagnosis of diseases related to vitamin D deficiency, such as osteoporosis, rickets, and other metabolic bone disorders.
Product Introduction

The 25-Hydroxy Vitamin D Assay Kit (Chemiluminescence Method) is a state-of-the-art diagnostic tool designed to accurately measure the levels of 25-hydroxy vitamin D in human serum and plasma. This kit is essential for assessing vitamin D status, which is crucial for bone health, immune function, and overall well-being. It employs a chemiluminescence-based method, ensuring high sensitivity and specificity in detecting vitamin D levels.

1. The kit features a highly sensitive chemiluminescence detection system, which ensures accurate and reliable results. The use of magnetic bead-coated particles enhances the binding efficiency of the antigen-antibody complex, improving the overall assay performance.
2. The kit's composition includes magnetic bead-coated particles, enzyme-labeled conjugates, reaction buffer, sample treatment solution, and optional calibrators and controls. Each component is meticulously formulated to ensure stability and reproducibility in various clinical settings.
3. The primary application of this kit is in the quantitative measurement of 25-hydroxy vitamin D levels in human serum and plasma. It is widely used in clinical laboratories to aid in the diagnosis and management of vitamin D deficiency-related conditions, such as osteoporosis and rickets.

The 25-Hydroxy Vitamin D Assay Kit, also known as the Vitamin D Test Kit, is a vital tool in modern clinical diagnostics. Its advanced chemiluminescence method and well-designed components make it a reliable choice for measuring vitamin D levels, helping healthcare providers make informed decisions about patient care.

Main Features

The 25-Hydroxy Vitamin D Assay Kit offers high sensitivity, specificity, and reproducibility in measuring vitamin D levels. Its advanced chemiluminescence method ensures accurate results, making it a trusted tool in clinical diagnostics.

1. The kit utilizes a chemiluminescence-based detection system, which provides high sensitivity and specificity in detecting 25-hydroxy vitamin D levels. This method ensures minimal interference from other substances in the sample.
2. The magnetic bead-coated particles enhance the binding efficiency of the antigen-antibody complex, leading to more accurate and reliable results. This feature is particularly beneficial in clinical settings where precision is paramount.
3. The kit includes a reaction buffer that stabilizes the assay conditions, ensuring consistent performance across different batches and laboratories. This stability is crucial for maintaining the reliability of the results.
4. The sample treatment solution is designed to prepare the serum or plasma samples effectively, ensuring that the 25-hydroxy vitamin D is accurately measured without interference from other components in the sample.
5. The optional calibrators and controls provide a means to validate the assay's performance, ensuring that the results are accurate and reproducible. This feature is essential for quality control in clinical laboratories.

The 25-Hydroxy Vitamin D Assay Kit stands out for its advanced chemiluminescence method, high sensitivity, and robust design. Its features ensure accurate and reliable measurement of vitamin D levels, making it an indispensable tool in clinical diagnostics.

Clinical Applications

The 25-Hydroxy Vitamin D Assay Kit is widely used in clinical settings to measure vitamin D levels, aiding in the diagnosis and management of vitamin D deficiency-related conditions such as osteoporosis, rickets, and metabolic bone disorders.

1. The kit is used in clinical laboratories to measure 25-hydroxy vitamin D levels in serum and plasma, providing critical information for the diagnosis of vitamin D deficiency.
2. It is particularly useful in managing patients with osteoporosis, as vitamin D levels are crucial for bone health and the prevention of fractures.
3. The kit is also employed in pediatric settings to diagnose and monitor rickets, a condition caused by severe vitamin D deficiency in children.
4. In metabolic bone disorders, the kit helps in assessing the effectiveness of vitamin D supplementation and other therapeutic interventions.
5. The kit's high sensitivity and specificity make it a valuable tool in research studies focused on the role of vitamin D in various health conditions, including immune function and chronic diseases.

The 25-Hydroxy Vitamin D Assay Kit is a versatile tool in clinical practice, aiding in the diagnosis and management of various vitamin D deficiency-related conditions. Its accuracy and reliability make it a trusted choice for healthcare providers.

Purchasing Guide

When purchasing the 25-Hydroxy Vitamin D Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your clinical or research needs.

1. Product specifications: Ensure the kit includes all necessary components, such as magnetic bead-coated particles, enzyme-labeled conjugates, reaction buffer, and sample treatment solution. Verify the presence of optional calibrators and controls for quality assurance.
2. Product types: Choose between different kit sizes and configurations based on your laboratory's throughput and testing volume. Consider whether you need additional components like calibrators and controls.
3. Budget: Evaluate the cost-effectiveness of the kit, considering the number of tests it can perform and the quality of the components. Ensure that the kit fits within your laboratory's budget without compromising on quality.
4. Intended use: Determine the specific clinical or research applications for which you need the kit. Ensure that the kit's sensitivity, specificity, and reproducibility align with your intended use.

Purchasing the 25-Hydroxy Vitamin D Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your clinical or research needs effectively.

 Precautions

When using the 25-Hydroxy Vitamin D Assay Kit, it is essential to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the components.

1. Handle all components with care, especially the magnetic bead-coated particles and enzyme-labeled conjugates, to avoid contamination and ensure accurate results.
2. Store the kit at the recommended temperature to maintain the stability and effectiveness of the components. Avoid exposure to extreme temperatures or direct sunlight.
3. Follow the manufacturer's instructions for sample preparation and assay procedures to ensure consistent and reliable results. Deviations from the protocol may lead to inaccurate measurements.
4. Use the optional calibrators and controls to validate the assay's performance and ensure that the results are accurate and reproducible. Regular quality control checks are essential for maintaining assay reliability.
5. Dispose of all used components and waste materials according to local regulations and laboratory safety protocols to prevent contamination and ensure a safe working environment.

Proper handling, storage, and usage of the 25-Hydroxy Vitamin D Assay Kit are crucial for obtaining accurate and reliable results. By following the recommended precautions, you can ensure the integrity of the assay and maintain a safe laboratory environment.

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