The β-Hydroxybutyrate (D3H) Assay Kit is a specialized diagnostic tool used to measure the concentration of β-Hydroxybutyrate in human serum. This kit employs the β-Hydroxybutyrate Dehydrogenase method, ensuring high accuracy and reliability. It is essential for diagnosing and monitoring metabolic conditions, especially in patients with diabetes or other metabolic disorders.

1. The kit features a highly sensitive enzymatic method, ensuring precise measurement of β-Hydroxybutyrate levels. The use of β-Hydroxybutyrate Dehydrogenase allows for specific detection, minimizing interference from other substances in the serum.
2. The composition includes two main reagents: Reagent 1 with Tris buffer and β-Hydroxybutyrate Dehydrogenase, and Reagent 2 containing Nicotinamide Adenine Dinucleotide and Oxalate. These components work together to facilitate the enzymatic reaction necessary for accurate measurement.
3. The primary use of this kit is in clinical settings for the quantitative determination of β-Hydroxybutyrate in serum. It is particularly useful in diagnosing diabetic ketoacidosis, monitoring metabolic health, and assessing the effectiveness of treatment in patients with metabolic disorders.

The β-Hydroxybutyrate (D3H) Assay Kit, also known as the D3H Assay Kit or β-HB Kit, is a vital tool in clinical diagnostics. Its precise enzymatic method and well-structured composition make it indispensable for measuring β-Hydroxybutyrate levels, particularly in metabolic disorder management.

The β-Hydroxybutyrate (D3H) Assay Kit offers high accuracy and reliability in measuring β-Hydroxybutyrate levels in serum. Its enzymatic method ensures specificity and sensitivity, making it a preferred choice in clinical diagnostics.

1. The kit utilizes a highly specific enzymatic reaction, ensuring accurate measurement of β-Hydroxybutyrate levels without interference from other serum components.
2. It includes a Tris buffer system that stabilizes the enzymatic reaction, enhancing the reliability of the results.
3. The presence of Nicotinamide Adenine Dinucleotide in Reagent 2 facilitates the enzymatic conversion of β-Hydroxybutyrate, ensuring precise quantification.
4. The kit is designed for ease of use, with clear instructions and pre-measured reagents, reducing the risk of user error.
5. It is compatible with a wide range of clinical analyzers, making it versatile and adaptable to various laboratory settings.

The β-Hydroxybutyrate (D3H) Assay Kit stands out for its precision, ease of use, and compatibility with clinical analyzers. Its enzymatic method and well-designed reagents make it a reliable tool for measuring β-Hydroxybutyrate levels in clinical settings.

The β-Hydroxybutyrate (D3H) Assay Kit is primarily used in clinical laboratories for the diagnosis and monitoring of metabolic disorders, particularly diabetic ketoacidosis.

1. It is used to diagnose diabetic ketoacidosis by measuring elevated levels of β-Hydroxybutyrate in serum, a key indicator of the condition.
2. The kit is employed in monitoring the effectiveness of treatment in patients with metabolic disorders, providing critical data for adjusting therapeutic strategies.
3. It is used in research settings to study metabolic pathways and the role of β-Hydroxybutyrate in various physiological and pathological conditions.
4. The kit is also utilized in emergency medicine to quickly assess the metabolic status of critically ill patients.
5. It is applied in routine health check-ups to screen for metabolic abnormalities, aiding in early detection and intervention.

The β-Hydroxybutyrate (D3H) Assay Kit is a versatile tool in clinical diagnostics, essential for diagnosing diabetic ketoacidosis, monitoring treatment, and researching metabolic disorders. Its applications extend to emergency medicine and routine health screenings, making it a valuable asset in healthcare.

When purchasing the β-Hydroxybutyrate (D3H) Assay Kit, consider product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Evaluate the product parameters, such as sensitivity, specificity, and compatibility with your laboratory equipment, to ensure accurate and reliable results.
2. Choose the appropriate type of kit based on your laboratory's volume and frequency of use. Bulk purchases may offer cost savings for high-volume labs.
3. Consider your budget, balancing cost with the need for high-quality reagents and reliable performance. Look for suppliers offering competitive pricing and bulk discounts.
4. Assess the intended use scenarios, such as routine diagnostics, emergency medicine, or research, to select a kit that aligns with your laboratory's specific requirements.

Purchasing the β-Hydroxybutyrate (D3H) Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure the kit meets your laboratory's needs and provides reliable results.

Proper handling and storage of the β-Hydroxybutyrate (D3H) Assay Kit are essential to ensure accurate results and maintain reagent stability.

1. Store the kit at the recommended temperature to preserve the integrity of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Follow the manufacturer's instructions for reagent preparation and usage to prevent contamination and ensure accurate measurements.
3. Use the kit within its expiration date to avoid compromised results. Discard any expired or visibly degraded reagents.
4. Ensure proper calibration of laboratory equipment before using the kit to maintain measurement accuracy.
5. Handle all reagents with care, wearing appropriate personal protective equipment to prevent exposure and ensure safety.

To ensure the reliability of the β-Hydroxybutyrate (D3H) Assay Kit, proper storage, handling, and usage are crucial. Following the manufacturer's guidelines and maintaining laboratory equipment will help achieve accurate and consistent results.