The β-Hydroxybutyrate Assay Kit (Enzymatic Method) is a sophisticated diagnostic tool used to measure the concentration of β-Hydroxybutyrate in human serum. This kit is essential for clinical laboratories as it provides a reliable and accurate method for detecting ketone bodies, which are critical indicators of metabolic health. The kit's enzymatic approach ensures high specificity and sensitivity, making it a preferred choice for healthcare professionals.

1. The β-Hydroxybutyrate Assay Kit is characterized by its high sensitivity and specificity, ensuring accurate detection of β-Hydroxybutyrate levels. The enzymatic method employed by the kit minimizes interference from other serum components, providing reliable results.
2. The kit consists of two reagents: Reagent 1 contains Tris-HCl buffer, β-Hydroxybutyrate dehydrogenase, and sodium azide, while Reagent 2 includes MES buffer, NAD+, sodium oxalate, and sodium azide. These components work synergistically to facilitate the enzymatic reaction necessary for β-Hydroxybutyrate measurement.
3. The primary use of this kit is for the quantitative determination of β-Hydroxybutyrate in human serum. It is particularly valuable in diagnosing and monitoring conditions such as diabetic ketoacidosis, where elevated ketone body levels are a key diagnostic marker.

The β-Hydroxybutyrate Assay Kit, also known as the Beta-HB Kit, is a vital tool in clinical diagnostics. Its enzymatic method ensures precise measurement of β-Hydroxybutyrate levels, making it indispensable for assessing metabolic health. The kit's robust composition and reliable performance make it a trusted choice for healthcare professionals.

The β-Hydroxybutyrate Assay Kit offers high sensitivity and specificity for detecting β-Hydroxybutyrate levels in human serum. Its enzymatic method ensures accurate and reliable results, making it a preferred choice for clinical laboratories.

1. High Sensitivity: The kit is designed to detect even low concentrations of β-Hydroxybutyrate, ensuring accurate diagnosis and monitoring of metabolic conditions.
2. Specificity: The enzymatic method minimizes interference from other serum components, providing reliable and specific results.
3. Ease of Use: The kit is user-friendly, with clear instructions and a straightforward protocol, making it accessible for laboratory technicians.
4. Stability: The reagents are stable under recommended storage conditions, ensuring consistent performance over time.
5. Comprehensive Kit: The kit includes all necessary components for the assay, reducing the need for additional reagents and simplifying the testing process.

The β-Hydroxybutyrate Assay Kit stands out for its high sensitivity, specificity, and ease of use. Its comprehensive design and stable reagents make it a reliable tool for clinical laboratories, ensuring accurate and consistent results for metabolic health assessments.

The β-Hydroxybutyrate Assay Kit is widely used in clinical settings for the diagnosis and monitoring of metabolic disorders, particularly diabetic ketoacidosis. Its accurate measurement of β-Hydroxybutyrate levels is crucial for effective patient management.

1. Diabetic Ketoacidosis: The kit is essential for diagnosing and monitoring diabetic ketoacidosis, a serious complication of diabetes characterized by elevated ketone body levels.
2. Starvation: It is used to assess ketone body levels in patients experiencing prolonged starvation, helping to guide nutritional interventions.
3. Metabolic Disorders: The kit aids in the diagnosis of various metabolic disorders where ketone body levels are elevated, providing critical information for treatment planning.
4. Emergency Medicine: In emergency settings, the kit is used to quickly assess ketone body levels in critically ill patients, aiding in rapid diagnosis and treatment.
5. Research: The kit is also used in research settings to study the role of ketone bodies in various metabolic processes and diseases.

The β-Hydroxybutyrate Assay Kit is a versatile tool in clinical practice, essential for diagnosing and monitoring metabolic disorders. Its applications range from emergency medicine to research, making it a valuable asset for healthcare professionals.

When purchasing the β-Hydroxybutyrate Assay Kit, it is important to consider product specifications, types, budget, and intended use to ensure the kit meets your laboratory's needs.

1. Product Parameters: Evaluate the kit's sensitivity, specificity, and stability to ensure it meets your laboratory's requirements for accurate and reliable results.
2. Product Types: Consider the different formats and sizes available to choose the one that best fits your laboratory's workflow and testing volume.
3. Budget: Assess the cost of the kit in relation to your laboratory's budget, ensuring it provides good value for the features and performance it offers.
4. Usage Scenarios: Determine the specific clinical or research applications for which the kit will be used, ensuring it is suitable for your intended purposes.

Purchasing the β-Hydroxybutyrate Assay Kit requires careful consideration of product parameters, types, budget, and usage scenarios. By evaluating these factors, you can ensure the kit meets your laboratory's needs and provides reliable results for your clinical or research applications.

When using the β-Hydroxybutyrate Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain reagent stability.

1. Storage: Store the kit at the recommended temperature to maintain reagent stability and ensure consistent performance.
2. Handling: Follow the manufacturer's instructions for handling the reagents to avoid contamination and ensure accurate results.
3. Calibration: Regularly calibrate the assay equipment to maintain accuracy and reliability of the results.
4. Safety: Use appropriate personal protective equipment when handling the reagents to avoid exposure to hazardous chemicals.
5. Disposal: Dispose of used reagents and materials according to local regulations to ensure environmental safety.

Proper handling, storage, and disposal of the β-Hydroxybutyrate Assay Kit are essential for maintaining reagent stability and ensuring accurate results. By following these precautions, you can ensure the kit performs reliably and safely in your laboratory.