The β-Hydroxybutyrate Assay Kit is a reliable and efficient tool for measuring β-hydroxybutyrate levels in human serum. Utilizing an enzymatic colorimetric method, it provides accurate and reproducible results, making it essential for clinical diagnostics and research. The kit includes all necessary reagents and an optional calibrator, ensuring ease of use and precision.

1. The kit employs a two-reagent system, with Reagent 1 containing a buffer, oxalate, NAD, and INT, and Reagent 2 containing a buffer, diaphorase, and β-hydroxybutyrate dehydrogenase. This combination ensures a stable and sensitive reaction for accurate measurement.
2. The enzymatic reaction involves the conversion of β-hydroxybutyrate to acetoacetate, coupled with the reduction of NAD to NADH. The INT in Reagent 1 reacts with NADH to produce a colored compound, which is measured spectrophotometrically.
3. This kit is specifically designed for clinical laboratories to monitor β-hydroxybutyrate levels, which are crucial in diagnosing and managing metabolic disorders such as diabetic ketoacidosis, starvation, and alcoholism.

The β-Hydroxybutyrate Assay Kit, also known as the BHB Assay Kit, is a vital tool in clinical diagnostics. Its two-reagent system and optional calibrator ensure precise and reliable measurements of β-hydroxybutyrate levels, making it indispensable for managing metabolic disorders.

The β-Hydroxybutyrate Assay Kit offers high sensitivity and specificity for measuring β-hydroxybutyrate levels. Its enzymatic colorimetric method ensures accurate and reproducible results, making it ideal for clinical and research applications.

1. The kit utilizes a two-reagent system, ensuring a stable and sensitive reaction for accurate measurement of β-hydroxybutyrate levels.
2. The enzymatic reaction involves the conversion of β-hydroxybutyrate to acetoacetate, coupled with the reduction of NAD to NADH, which is then measured spectrophotometrically.
3. The kit includes an optional calibrator, providing a reference for accurate quantification and ensuring consistency across different batches.
4. The assay is designed for high-throughput applications, making it suitable for use in clinical laboratories with large sample volumes.
5. The kit is compatible with most automated analyzers, offering flexibility and ease of integration into existing laboratory workflows.

The β-Hydroxybutyrate Assay Kit stands out for its high sensitivity, specificity, and ease of use. Its two-reagent system and optional calibrator ensure precise measurements, making it a reliable choice for clinical diagnostics and research.

The β-Hydroxybutyrate Assay Kit is widely used in clinical settings to monitor β-hydroxybutyrate levels, which are critical in diagnosing and managing metabolic disorders such as diabetic ketoacidosis, starvation, and alcoholism.

1. The kit is essential for diagnosing diabetic ketoacidosis, a life-threatening condition characterized by elevated ketone body levels, including β-hydroxybutyrate.
2. It is used to monitor patients with type 1 diabetes, helping to prevent complications associated with high ketone levels.
3. The kit is also valuable in assessing nutritional status, particularly in cases of starvation or extreme dieting, where ketone body levels may rise.
4. In cases of alcoholism, the kit helps in monitoring ketone body levels, which can be elevated due to impaired liver function.
5. The assay is also used in research settings to study metabolic pathways and the role of ketone bodies in various physiological and pathological conditions.

The β-Hydroxybutyrate Assay Kit is a versatile tool in clinical diagnostics, essential for managing conditions like diabetic ketoacidosis, starvation, and alcoholism. Its ability to provide accurate and reliable measurements makes it indispensable in both clinical and research settings.

When purchasing the β-Hydroxybutyrate Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product specifications: Ensure the kit includes all necessary reagents and an optional calibrator for accurate quantification.
2. Product types: Choose between manual and automated kits based on your laboratory's workflow and sample volume.
3. Budget: Consider the cost per test and the overall budget for your laboratory to ensure cost-effectiveness.
4. Intended use: Evaluate the kit's compatibility with your laboratory's equipment and its suitability for your specific clinical or research applications.

Purchasing the β-Hydroxybutyrate Assay Kit requires careful consideration of product specifications, types, budget, and intended use. Ensuring these factors align with your laboratory's needs will guarantee optimal performance and cost-effectiveness.

When using the β-Hydroxybutyrate Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate and reliable results.

1. Store the kit at the recommended temperature to maintain the stability and efficacy of the reagents.
2. Follow the manufacturer's instructions for reagent preparation and assay procedure to avoid errors.
3. Use appropriate protective equipment, such as gloves and lab coats, when handling reagents to prevent contamination and ensure safety.
4. Regularly calibrate the spectrophotometer or analyzer used with the kit to ensure accurate measurements.
5. Dispose of used reagents and samples according to local regulations and laboratory safety protocols.

Proper handling and storage of the β-Hydroxybutyrate Assay Kit are crucial for obtaining accurate and reliable results. Following the manufacturer's instructions and adhering to safety protocols will ensure optimal performance and safety in the laboratory.