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β-Hydroxybutyrate Assay Kit (Enzymatic Method)
The β-Hydroxybutyrate Assay Kit is composed of two main reagents, R1 and R2. R1 contains Tris(hydroxymethyl)aminomethane at 0.1mol/L, β-Hydroxybutyrate Dehydrogenase at 0.18KU/L, and Diaphorase at 1KU/L. R2 includes Tris(hydroxymethyl)aminomethane at 0.1mol/L, Oxidized Nicotinamide Adenine Dinucleotide at 3.0mmol/L, Iodonitrotetrazolium Chloride at 20mmol/L, and Ammonium Oxalate at 20mmol/L. It is important to note that components from different batch numbers are not interchangeable.
Product English Name:
β-Hydroxybutyrate Assay Kit (Enzymatic Method)
Alias:
D3H Assay Kit,Ketone Body Test Kit
Intended Use:
This kit is designed for medical institutions to quantitatively measure the concentration of β-Hydroxybutyrate (D3H) in human serum or plasma samples. It serves as an auxiliary diagnostic tool for conditions related to ketone body metabolism.
Product Introduction

The β-Hydroxybutyrate Assay Kit (Enzymatic Method) is a specialized diagnostic tool used in clinical laboratories to measure the levels of β-Hydroxybutyrate, a key ketone body, in human serum or plasma. This kit is essential for diagnosing and monitoring conditions such as diabetic ketoacidosis, starvation, and other metabolic disorders. The enzymatic method ensures high specificity and sensitivity, making it a reliable choice for healthcare professionals.

1. The kit features a highly specific enzymatic reaction that targets β-Hydroxybutyrate, ensuring accurate and reliable results. The use of β-Hydroxybutyrate Dehydrogenase and Diaphorase in the reaction mixture enhances the sensitivity of the assay.
2. The composition of the kit includes two main reagents, R1 and R2, each containing specific components that facilitate the enzymatic reaction. The precise concentration of each component is critical for the assay's performance.
3. The primary use of this kit is to assist in the diagnosis of metabolic disorders by measuring the concentration of β-Hydroxybutyrate in serum or plasma. It is particularly useful in managing diabetic patients and those experiencing ketosis.

The β-Hydroxybutyrate Assay Kit, also known as the D3H Assay Kit, is a vital tool in clinical diagnostics. Its enzymatic method ensures precise measurement of β-Hydroxybutyrate levels, aiding in the diagnosis and management of metabolic disorders. The kit's specific composition and high sensitivity make it an indispensable resource for healthcare providers.

Main Features

The β-Hydroxybutyrate Assay Kit offers high specificity and sensitivity in measuring β-Hydroxybutyrate levels, making it an essential tool for diagnosing metabolic disorders.

1. The kit utilizes a highly specific enzymatic reaction to measure β-Hydroxybutyrate levels accurately.
2. It includes two main reagents, R1 and R2, each with precise concentrations of enzymes and co-factors necessary for the reaction.
3. The assay is designed for use with human serum or plasma, providing reliable results for clinical diagnostics.
4. The kit is particularly useful in managing diabetic patients and those experiencing ketosis, offering quick and accurate results.
5. The enzymatic method ensures minimal interference from other substances, enhancing the reliability of the assay.

The β-Hydroxybutyrate Assay Kit stands out for its high specificity and sensitivity, making it a crucial tool in clinical diagnostics. Its enzymatic method ensures accurate measurement of β-Hydroxybutyrate levels, aiding in the diagnosis and management of metabolic disorders. The kit's precise composition and reliable performance make it a trusted choice for healthcare professionals.

Clinical Applications

The β-Hydroxybutyrate Assay Kit is widely used in clinical settings to diagnose and monitor metabolic disorders, particularly diabetic ketoacidosis and ketosis.

1. The kit is used in hospitals and clinical laboratories to measure β-Hydroxybutyrate levels in patients with suspected metabolic disorders.
2. It is particularly useful in emergency settings for rapid diagnosis of diabetic ketoacidosis.
3. The assay aids in monitoring the effectiveness of treatment in patients with metabolic disorders.
4. It is also used in research settings to study ketone body metabolism and related conditions.
5. The kit's high sensitivity and specificity make it a valuable tool for both diagnostic and research purposes.

The β-Hydroxybutyrate Assay Kit is a versatile tool in clinical diagnostics, widely used for diagnosing and monitoring metabolic disorders. Its high sensitivity and specificity make it particularly valuable in emergency settings and for research purposes. The kit's reliable performance ensures accurate results, aiding in effective patient management.

Purchasing Guide

When purchasing the β-Hydroxybutyrate Assay Kit, consider product parameters, types, budget, and usage scenarios to ensure it meets your clinical needs.

1. Evaluate the product parameters, such as sensitivity, specificity, and shelf life, to ensure they meet your laboratory's requirements.
2. Consider the different types of assay kits available, including those with varying reagent compositions, to find the best fit for your diagnostic needs.
3. Assess your budget and compare prices from different suppliers to ensure cost-effectiveness without compromising quality.
4. Determine the specific usage scenarios, such as emergency diagnostics or routine monitoring, to select the most appropriate kit.

Purchasing the β-Hydroxybutyrate Assay Kit requires careful consideration of product parameters, types, budget, and usage scenarios. Ensuring the kit meets your clinical needs will enhance diagnostic accuracy and patient care. By evaluating these factors, you can select the most suitable kit for your laboratory.

 Precautions

Proper handling and storage of the β-Hydroxybutyrate Assay Kit are essential to ensure accurate results and maintain the integrity of the reagents.

1. Always store the kit at the recommended temperature to prevent degradation of the reagents.
2. Ensure that the reagents are mixed thoroughly before use to achieve consistent results.
3. Avoid using reagents from different batch numbers, as this can affect the accuracy of the assay.
4. Follow the manufacturer's instructions carefully to avoid contamination and ensure proper handling of the samples.
5. Dispose of used reagents and samples according to local regulations to maintain a safe laboratory environment.

Handling the β-Hydroxybutyrate Assay Kit with care is crucial for obtaining accurate results. Proper storage, thorough mixing of reagents, and adherence to manufacturer instructions are essential. By following these precautions, you can ensure the reliability of the assay and maintain a safe laboratory environment.

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