The β-Hydroxybutyrate Assay Kit (Enzymatic Colorimetric Method) is a diagnostic tool used to measure the concentration of β-hydroxybutyrate in human serum. This kit is essential for diagnosing and monitoring conditions related to ketone body metabolism, such as diabetic ketoacidosis. The assay is based on an enzymatic reaction that produces a color change, which can be measured spectrophotometrically to determine the concentration of β-hydroxybutyrate.

1. The kit features a highly specific enzymatic reaction that ensures accurate measurement of β-hydroxybutyrate levels. The reaction is catalyzed by β-hydroxybutyrate dehydrogenase, which converts β-hydroxybutyrate to acetoacetate, producing NADH in the process.
2. The composition of the kit includes two reagents: Reagent R1 and Reagent R2. Reagent R1 contains Tris buffer, oxalate, NAD+, diaphorase, and INT, while Reagent R2 contains β-hydroxybutyrate dehydrogenase. These components work synergistically to facilitate the enzymatic reaction.
3. The primary use of this kit is for the quantitative determination of β-hydroxybutyrate in human serum. It is particularly useful in clinical settings for diagnosing and monitoring metabolic disorders, such as diabetic ketoacidosis, where elevated levels of ketone bodies are present.

The β-Hydroxybutyrate Assay Kit, also known as the BHB Assay Kit, is a vital tool in clinical diagnostics. It provides a reliable method for measuring β-hydroxybutyrate levels in human serum, which is crucial for identifying metabolic disorders. The kit's enzymatic colorimetric method ensures accuracy and specificity, making it a trusted choice for healthcare professionals.

The β-Hydroxybutyrate Assay Kit offers a precise and reliable method for measuring β-hydroxybutyrate levels in human serum. Its enzymatic colorimetric approach ensures high specificity and accuracy, making it an essential tool in clinical diagnostics.

1. The kit utilizes a highly specific enzymatic reaction to measure β-hydroxybutyrate levels. This reaction is catalyzed by β-hydroxybutyrate dehydrogenase, ensuring accurate results.
2. The assay is designed to be user-friendly, with a straightforward protocol that can be easily followed in clinical laboratories. The colorimetric method allows for quick and reliable results.
3. The kit includes two reagents, Reagent R1 and Reagent R2, which are optimized to work together to facilitate the enzymatic reaction. This ensures consistent performance and reliable results.
4. The assay is highly sensitive, capable of detecting low levels of β-hydroxybutyrate in serum samples. This sensitivity is crucial for early detection of metabolic disorders.
5. The kit is compatible with a wide range of spectrophotometers, making it versatile and easy to integrate into existing laboratory workflows.

The β-Hydroxybutyrate Assay Kit stands out for its precision, ease of use, and reliability. Its enzymatic colorimetric method ensures accurate measurement of β-hydroxybutyrate levels, making it an indispensable tool in clinical diagnostics. Whether you're monitoring diabetic ketoacidosis or other metabolic disorders, this kit provides the accuracy and reliability you need.

The β-Hydroxybutyrate Assay Kit is widely used in clinical settings for the diagnosis and monitoring of metabolic disorders, particularly diabetic ketoacidosis. Its ability to accurately measure β-hydroxybutyrate levels makes it a valuable tool for healthcare professionals.

1. The kit is primarily used in the diagnosis of diabetic ketoacidosis, a serious complication of diabetes characterized by elevated levels of ketone bodies, including β-hydroxybutyrate.
2. It is also used in the monitoring of patients with diabetes to assess the effectiveness of treatment and to prevent complications related to ketone body accumulation.
3. The assay is valuable in emergency settings, where rapid and accurate measurement of β-hydroxybutyrate levels is crucial for patient management.
4. In addition to diabetes, the kit can be used to diagnose other metabolic disorders that result in elevated ketone body levels, such as starvation or alcoholism.
5. The kit is also used in research settings to study the role of ketone bodies in various metabolic processes and diseases.

The β-Hydroxybutyrate Assay Kit is a versatile tool in clinical diagnostics, particularly for the diagnosis and monitoring of diabetic ketoacidosis. Its ability to provide accurate and rapid results makes it invaluable in both clinical and research settings. Whether you're managing a patient in an emergency or conducting research on metabolic disorders, this kit offers the precision and reliability you need.

When purchasing the β-Hydroxybutyrate Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide will help you make an informed decision.

1. Product specifications: Ensure that the kit meets your laboratory's requirements in terms of sensitivity, specificity, and compatibility with your existing equipment.
2. Product types: There are different types of β-hydroxybutyrate assay kits available, including those designed for specific applications or with additional features. Choose the one that best fits your needs.
3. Budget: Consider the cost of the kit, including any additional reagents or equipment that may be required. Ensure that the kit fits within your laboratory's budget.
4. Intended use: Determine the primary use of the kit, whether for clinical diagnostics, research, or other applications. This will help you select the most appropriate kit for your needs.

Purchasing the right β-Hydroxybutyrate Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you select a kit that meets your laboratory's needs and provides reliable results.

When using the β-Hydroxybutyrate Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Always handle the reagents with care, avoiding contamination. Use clean, sterile pipettes and containers to prevent cross-contamination.
2. Store the kit according to the manufacturer's instructions, typically at 2-8°C. Do not freeze the reagents, as this may affect their performance.
3. Ensure that all equipment, including spectrophotometers and pipettes, is properly calibrated before use. This will help ensure accurate and reliable results.
4. Follow the assay protocol carefully, including incubation times and reagent volumes. Deviations from the protocol may result in inaccurate results.
5. Dispose of used reagents and samples according to local regulations and laboratory safety protocols. This will help prevent contamination and ensure a safe working environment.

Proper handling and storage of the β-Hydroxybutyrate Assay Kit are essential for obtaining accurate and reliable results. By following the recommended precautions and protocols, you can ensure the integrity of the reagents and maintain a safe laboratory environment. Always handle the kit with care and adhere to safety guidelines to achieve the best possible outcomes.