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β-Hydroxybutyrate Assay Kit (Enzymatic Method)
The β-Hydroxybutyrate Assay Kit is composed of two main reagents: Reagent R1 and Reagent R2. Reagent R1 contains β-Hydroxybutyrate Dehydrogenase (2.3 KU/L), PIPES buffer (50 mmol/L), and Diaphorase (2 KU/L). Reagent R2 includes NAD (0.7 mmol/L), Tartaric Acid (7.5 mmol/L), and INT (0.354 g/L). Additionally, the kit includes a calibrator and a control, both containing 3-Hydroxybutyrate Sodium and a buffer solution.
Product English Name:
β-Hydroxybutyrate Assay Kit (Enzymatic Method)
Alias:
β-HB Test Kit,Ketone Body Assay Kit
Intended Use:
This kit is designed for the quantitative determination of β-Hydroxybutyrate (β-HB) levels in serum or plasma samples. It is widely used in clinical laboratories to assess ketone body levels, which are crucial for diagnosing and monitoring conditions such as diabetic ketoacidosis and other metabolic disorders.
Product Introduction

The β-Hydroxybutyrate Assay Kit (Enzymatic Method) is a highly sensitive and specific tool for measuring β-Hydroxybutyrate levels in biological samples. It employs an enzymatic reaction that is both rapid and reliable, making it an essential component in clinical diagnostics. The kit is designed to provide accurate results, ensuring that healthcare professionals can make informed decisions based on the data obtained.

1. The kit features a robust enzymatic method that ensures high specificity and sensitivity in detecting β-Hydroxybutyrate levels. This method is particularly advantageous in clinical settings where accuracy is paramount.
2. The composition of the kit includes two main reagents, a calibrator, and a control. Each component is carefully formulated to ensure stability and reliability during the assay process.
3. The primary use of this kit is in the quantitative determination of β-Hydroxybutyrate in serum or plasma. This measurement is critical for diagnosing and managing conditions such as diabetic ketoacidosis, where ketone body levels are elevated.

The β-Hydroxybutyrate Assay Kit, also known as the β-HB Test Kit, is a vital tool in clinical diagnostics. Its enzymatic method ensures precise measurements, making it indispensable for healthcare professionals. The kit's composition and design are tailored to provide reliable results, aiding in the effective management of metabolic disorders.

Main Features

The β-Hydroxybutyrate Assay Kit offers a range of features that make it a preferred choice for clinical laboratories. Its enzymatic method ensures high accuracy and specificity, while its stable reagents provide consistent results.

1. The kit utilizes a highly specific enzymatic reaction to detect β-Hydroxybutyrate, ensuring minimal interference from other substances in the sample.
2. The reagents are formulated to maintain stability over a wide range of storage conditions, ensuring reliable performance even after prolonged storage.
3. The kit includes a calibrator and control, which are essential for validating the accuracy of the assay and ensuring consistent results across different batches.
4. The assay is designed to be user-friendly, with a straightforward protocol that can be easily followed by laboratory personnel.
5. The kit is compatible with a variety of automated analyzers, making it suitable for high-throughput laboratories.

The β-Hydroxybutyrate Assay Kit stands out for its accuracy, stability, and ease of use. Its enzymatic method and well-formulated reagents make it a reliable choice for clinical diagnostics, particularly in the management of metabolic disorders.

Clinical Applications

The β-Hydroxybutyrate Assay Kit is widely used in clinical settings to measure ketone body levels, which are critical for diagnosing and monitoring conditions such as diabetic ketoacidosis.

1. The kit is used in the diagnosis of diabetic ketoacidosis, where elevated levels of β-Hydroxybutyrate are a key indicator.
2. It is also employed in the monitoring of patients with diabetes, helping to assess the effectiveness of treatment and prevent complications.
3. The assay is useful in emergency settings, where rapid and accurate measurement of ketone bodies is essential for patient management.
4. It is used in research studies to investigate the role of ketone bodies in various metabolic processes and diseases.
5. The kit is also applied in veterinary medicine, where it helps in the diagnosis and management of metabolic disorders in animals.

The β-Hydroxybutyrate Assay Kit is a versatile tool in clinical diagnostics, with applications ranging from the diagnosis of diabetic ketoacidosis to research studies. Its accuracy and reliability make it an essential component in the management of metabolic disorders.

Purchasing Guide

When purchasing the β-Hydroxybutyrate Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use to ensure that the kit meets your laboratory's needs.

1. Product specifications: Ensure that the kit's sensitivity, specificity, and range of detection align with your laboratory's requirements.
2. Product types: Consider whether you need a manual or automated version of the kit, depending on your laboratory's workflow and equipment.
3. Budget: Evaluate the cost of the kit in relation to its features and the volume of tests you plan to perform.
4. Usage scenarios: Determine the specific applications for which you will use the kit, such as clinical diagnostics or research, to ensure it is suitable for your needs.

Purchasing the β-Hydroxybutyrate Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that the kit meets your laboratory's needs and provides reliable results.

 Precautions

When using the β-Hydroxybutyrate Assay Kit, it is important to follow the manufacturer's instructions carefully to ensure accurate and reliable results. Proper handling and storage of the reagents are also crucial.

1. Always use the kit within its expiration date to ensure the stability and effectiveness of the reagents.
2. Store the reagents at the recommended temperature to prevent degradation and maintain their performance.
3. Follow the assay protocol precisely, including the correct order of reagent addition and incubation times, to avoid errors in the results.
4. Use the provided calibrator and control to validate the accuracy of the assay and ensure consistent results.
5. Dispose of used reagents and samples according to local regulations to prevent contamination and ensure safety.

Proper handling and use of the β-Hydroxybutyrate Assay Kit are essential for obtaining accurate and reliable results. By following the manufacturer's instructions and taking necessary precautions, you can ensure the kit's effectiveness and maintain a safe laboratory environment.

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