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Alpha-Hydroxybutyrate Dehydrogenase Assay Kit (DGKC Method)
The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit is composed of two main reagents: R1, which includes Tris(hydroxymethyl)aminomethane buffer, stabilizers, and preservatives; and R2, which contains α-ketobutyrate and reduced nicotinamide adenine dinucleotide (NADH). This kit is designed for the quantitative determination of α-hydroxybutyrate dehydrogenase activity in human serum or plasma.
Product English Name:
Alpha-Hydroxybutyrate Dehydrogenase Assay Kit (DGKC Method)
Alias:
HBDH Assay Kit,α-HBDH Kit
Intended Use:
This kit is used for the in vitro quantitative determination of α-hydroxybutyrate dehydrogenase (α-HBDH) activity in human serum or plasma. It is commonly employed in clinical laboratories to assess myocardial damage, as α-HBDH is a marker for heart-related conditions.
Product Introduction

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit (DGKC Method) is a specialized diagnostic tool used to measure the activity of α-HBDH in human serum or plasma. This enzyme is a key indicator of myocardial damage, making the kit essential in cardiology diagnostics. The kit includes two reagents that work together to provide accurate and reliable results, ensuring that healthcare professionals can make informed decisions based on the data obtained.

1. The kit is designed with precision in mind, offering high sensitivity and specificity for α-HBDH detection. It is optimized for use in clinical laboratories, ensuring consistent performance across different testing environments.
2. The composition of the kit includes R1, which contains a Tris buffer, stabilizers, and preservatives to maintain reagent integrity, and R2, which includes α-ketobutyrate and NADH, essential for the enzymatic reaction.
3. The primary use of the kit is to measure α-HBDH activity, which is crucial for diagnosing myocardial infarction and other heart-related conditions. It is widely used in clinical settings to monitor patient health and guide treatment decisions.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a vital tool in clinical diagnostics. Its precise composition and reliable performance make it indispensable for assessing heart health. Whether you're a clinician or a lab technician, this kit provides the accuracy and consistency needed for effective patient care.

Main Features

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit offers high accuracy and reliability in measuring α-HBDH activity. It is designed for ease of use in clinical laboratories, providing consistent results for diagnosing myocardial damage.

1. The kit features a two-reagent system that ensures stability and accuracy in the enzymatic reaction, making it suitable for high-throughput clinical testing.
2. It includes a Tris buffer in R1, which maintains the pH stability necessary for the enzymatic reaction, along with stabilizers and preservatives to extend the shelf life of the reagents.
3. R2 contains α-ketobutyrate and NADH, which are essential for the accurate measurement of α-HBDH activity. The combination of these components ensures precise and reproducible results.
4. The kit is optimized for use with automated analyzers, allowing for efficient and consistent testing in busy clinical laboratories.
5. It provides a wide dynamic range for α-HBDH measurement, making it suitable for detecting both normal and elevated levels of the enzyme in patient samples.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit is a reliable and efficient tool for measuring α-HBDH activity. Its robust design and high accuracy make it a preferred choice for clinical laboratories, ensuring that healthcare providers can trust the results for critical diagnostic decisions.

Clinical Applications

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings to diagnose and monitor myocardial infarction and other heart-related conditions. It is a key tool in cardiology diagnostics.

1. The kit is used to measure α-HBDH activity in serum or plasma, which is a marker for myocardial damage. This is crucial for diagnosing acute myocardial infarction.
2. It is employed in routine clinical testing to monitor patients with suspected heart conditions, providing valuable data for treatment planning.
3. The kit is also used in research settings to study the role of α-HBDH in various cardiovascular diseases, contributing to a better understanding of heart health.
4. In emergency medicine, the kit is used to quickly assess myocardial damage in patients presenting with chest pain, aiding in rapid diagnosis and treatment.
5. The kit is integrated into automated laboratory systems, allowing for high-throughput testing and efficient patient management in large healthcare facilities.

The Alpha-Hydroxybutyrate Dehydrogenase Assay Kit is an essential tool in clinical diagnostics, particularly in cardiology. Its ability to accurately measure α-HBDH activity makes it invaluable for diagnosing and monitoring heart-related conditions, ensuring that patients receive timely and appropriate care.

Purchasing Guide

When purchasing the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit, consider factors such as product specifications, intended use, budget, and compatibility with existing laboratory equipment.

1. Evaluate the product parameters, including sensitivity, specificity, and dynamic range, to ensure the kit meets your diagnostic needs.
2. Consider the types of samples the kit is designed for (e.g., serum or plasma) and ensure it aligns with your laboratory's testing requirements.
3. Assess your budget and compare prices from different suppliers to find a cost-effective solution without compromising on quality.
4. Determine the intended use of the kit, whether for routine clinical testing, research, or emergency diagnostics, and ensure it is suitable for your specific application.

Purchasing the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of various factors, including product specifications, budget, and intended use. By taking these into account, you can ensure that you select the right kit for your laboratory's needs.

 Precautions

When using the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain reagent integrity.

1. Always store the kit at the recommended temperature to preserve the stability of the reagents and ensure consistent performance.
2. Follow the manufacturer's instructions for reagent preparation and handling to avoid contamination and ensure accurate results.
3. Use appropriate personal protective equipment (PPE) when handling the kit to protect against potential hazards and ensure safety in the laboratory.
4. Regularly calibrate and maintain laboratory equipment used with the kit to ensure accurate and reliable test results.
5. Dispose of used reagents and samples according to local regulations and laboratory safety protocols to minimize environmental impact and ensure compliance.

Proper handling and storage of the Alpha-Hydroxybutyrate Dehydrogenase Assay Kit are essential for maintaining reagent integrity and ensuring accurate results. By following the recommended precautions, you can ensure the safety and reliability of your diagnostic testing.

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