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α-Hydroxybutyrate Dehydrogenase Assay Kit (α-Ketobutyrate Substrate Method)
The kit consists of two main reagents: Reagent 1 contains reduced nicotinamide adenine dinucleotide (NADH), and Reagent 2 includes a phosphate buffer (pH 7.5) and α-ketobutyrate. These components are essential for the accurate measurement of α-hydroxybutyrate dehydrogenase activity in human serum.
Product English Name:
α-Hydroxybutyrate Dehydrogenase Assay Kit (α-Ketobutyrate Substrate Method)
Alias:
HBDH Assay Kit,α-HBDH Test Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of α-hydroxybutyrate dehydrogenase (α-HBDH) activity in human serum. It is widely used in clinical laboratories to assess cardiac and liver function, as α-HBDH levels are indicative of tissue damage.
Product Introduction

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a specialized diagnostic tool used to measure the activity of α-HBDH in human serum. This enzyme is a key marker in assessing tissue damage, particularly in the heart and liver. The kit provides a reliable and accurate method for clinical laboratories to perform this essential test.

1. The kit features a highly sensitive and specific assay method, ensuring precise measurement of α-HBDH activity. It is designed to minimize interference from other serum components, providing consistent and reproducible results.
2. The composition of the kit includes two reagents: Reagent 1 contains NADH, which is crucial for the enzymatic reaction, and Reagent 2 consists of a phosphate buffer and α-ketobutyrate, which together create the optimal conditions for the assay.
3. The primary use of this kit is in clinical diagnostics, where it aids in the evaluation of cardiac and liver health. By measuring α-HBDH levels, healthcare providers can detect and monitor tissue damage, guiding treatment decisions.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a vital tool in clinical diagnostics. Its precise measurement of α-HBDH activity helps in assessing tissue damage, particularly in the heart and liver. The kit's robust design and reliable performance make it an indispensable resource for healthcare professionals.

Main Features

The main features of the α-Hydroxybutyrate Dehydrogenase Assay Kit include high sensitivity, specificity, and reliability in measuring α-HBDH activity. It is designed for ease of use in clinical laboratories, providing accurate results essential for patient care.

1. High Sensitivity: The kit is capable of detecting even low levels of α-HBDH activity, making it suitable for early detection of tissue damage.
2. Specificity: The assay is designed to specifically measure α-HBDH without interference from other serum enzymes, ensuring accurate results.
3. Reliability: The kit provides consistent and reproducible results, which are crucial for clinical decision-making.
4. Ease of Use: The straightforward protocol allows for quick and efficient testing in clinical laboratories.
5. Comprehensive: The kit includes all necessary reagents and detailed instructions, making it a complete solution for α-HBDH measurement.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its high sensitivity, specificity, and reliability. Its ease of use and comprehensive nature make it an essential tool for clinical laboratories, ensuring accurate and timely results for patient care.

Clinical Applications

The α-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings to assess cardiac and liver function. It is a critical tool in diagnosing and monitoring tissue damage, particularly in patients with heart or liver conditions.

1. Cardiac Assessment: The kit is used to measure α-HBDH levels in patients suspected of myocardial infarction, helping to confirm the diagnosis and monitor recovery.
2. Liver Function: Elevated α-HBDH levels can indicate liver damage, making this kit useful in diagnosing and managing liver diseases.
3. Monitoring Treatment: The kit is used to track the effectiveness of treatments for heart and liver conditions, providing valuable feedback for healthcare providers.
4. Research Applications: Beyond clinical use, the kit is also employed in research studies to investigate the role of α-HBDH in various diseases.
5. Routine Screening: The kit is used in routine health check-ups to screen for early signs of tissue damage, particularly in high-risk patients.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, used for assessing cardiac and liver function, monitoring treatment, and conducting research. Its applications extend to routine screening, making it a valuable resource for healthcare providers.

Purchasing Guide

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to consider product specifications, types, budget, and intended use. This guide provides essential information to help make an informed decision.

1. Product Specifications: Ensure the kit meets the required sensitivity, specificity, and reliability standards for your laboratory's needs.
2. Product Types: Choose between different kit formats, such as single-use or bulk packs, based on your laboratory's testing volume.
3. Budget: Consider the cost per test and overall budget constraints when selecting a kit.
4. Intended Use: Determine the specific clinical or research applications for which the kit will be used to ensure it meets your requirements.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, types, budget, and intended use. This guide aims to assist in making an informed decision that aligns with your laboratory's needs.

 Precautions

Proper handling and storage of the α-Hydroxybutyrate Dehydrogenase Assay Kit are essential to ensure accurate results and maintain reagent integrity. This section outlines key precautions to follow.

1. Storage Conditions: Store the kit at the recommended temperature to preserve reagent stability and ensure accurate results.
2. Handling: Use aseptic techniques when handling reagents to prevent contamination, which could affect test outcomes.
3. Expiration Date: Always check the expiration date before use to ensure the reagents are still effective.
4. Calibration: Regularly calibrate the equipment used with the kit to maintain accuracy and reliability.
5. Disposal: Follow proper disposal procedures for used reagents and materials to comply with safety and environmental regulations.

Adhering to proper storage, handling, and disposal practices is crucial when using the α-Hydroxybutyrate Dehydrogenase Assay Kit. These precautions help ensure accurate results and maintain the integrity of the reagents, ultimately supporting effective patient care.

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