The α-Hydroxybutyrate Dehydrogenase (HBDH) Assay Kit (UV Kinetic Method) is a specialized diagnostic tool used to measure the activity of the HBDH enzyme in human serum and plasma. This enzyme is crucial in metabolic processes, and its levels can provide valuable insights into cardiac and hepatic health. The kit employs a UV kinetic method, ensuring high sensitivity and precision in enzyme activity measurement.

1. The kit features a dual-reagent system, which enhances the accuracy and stability of the assay. The reagents are optimized for consistent performance, ensuring reliable results across different sample types.
2. The composition includes a phosphate buffer and α-ketobutyrate in R1, and NADH in R2. These components work synergistically to facilitate the enzymatic reaction, allowing for precise measurement of HBDH activity.
3. The primary use of this kit is in clinical diagnostics, where it aids in the assessment of enzyme levels in serum and plasma. This information is vital for diagnosing and monitoring conditions related to cardiac and hepatic function.

The α-Hydroxybutyrate Dehydrogenase (HBDH) Assay Kit, also known as the HBDH Kit, is a reliable diagnostic tool for measuring enzyme activity. Its dual-reagent system and optimized composition make it a preferred choice in clinical settings for assessing metabolic health.

The HBDH Assay Kit offers high sensitivity and precision in measuring enzyme activity, making it an essential tool in clinical diagnostics.

1. The kit employs a UV kinetic method, which ensures accurate and reproducible results. This method is particularly effective in detecting low levels of enzyme activity.
2. The dual-reagent system enhances the stability and reliability of the assay. The reagents are designed to work together seamlessly, minimizing variability in results.
3. The kit is optimized for use with human serum and plasma, making it suitable for a wide range of clinical applications. It provides consistent performance across different sample types.
4. The assay is easy to perform, with clear instructions provided. This makes it accessible to both experienced and novice users in clinical laboratories.
5. The kit is designed for high-throughput applications, allowing for the simultaneous analysis of multiple samples. This increases efficiency in busy clinical settings.

The HBDH Assay Kit stands out for its precision, ease of use, and reliability. Its dual-reagent system and UV kinetic method make it a valuable tool in clinical diagnostics, particularly for assessing enzyme activity in serum and plasma.

The HBDH Assay Kit is widely used in clinical settings to diagnose and monitor conditions related to cardiac and hepatic health.

1. The kit is used to measure HBDH activity in serum and plasma, which is crucial for diagnosing myocardial infarction. Elevated levels of HBDH can indicate heart muscle damage.
2. It is also employed in the assessment of liver function. Abnormal HBDH levels can signal hepatic disorders, aiding in the diagnosis and monitoring of liver diseases.
3. The kit is used in research settings to study metabolic pathways and enzyme kinetics. Its precision and reliability make it a valuable tool for scientific investigations.
4. In clinical laboratories, the kit is used for routine testing of enzyme activity. Its high-throughput capability allows for efficient analysis of multiple samples.
5. The kit is also used in veterinary medicine to assess enzyme activity in animal serum and plasma, providing insights into animal health and disease.

The HBDH Assay Kit is a versatile tool in clinical diagnostics, used for assessing cardiac and hepatic health. Its applications extend to research and veterinary medicine, making it a valuable asset in various fields.

When purchasing the HBDH Assay Kit, consider product specifications, types, budget, and intended use to ensure it meets your needs.

1. Product parameters such as sensitivity, precision, and shelf life should be evaluated. Ensure the kit meets the required standards for your laboratory.
2. Different types of kits may be available, including those for specific sample types or high-throughput applications. Choose the one that best fits your laboratory's needs.
3. Budget considerations are important. Compare prices from different suppliers, but also consider the quality and reliability of the product.
4. The intended use of the kit, whether for routine testing, research, or veterinary applications, should guide your purchase decision. Ensure the kit is suitable for your specific requirements.

Purchasing the HBDH Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable results.

Proper handling and storage of the HBDH Assay Kit are essential to ensure accurate results and prolong its shelf life.

1. Store the kit at the recommended temperature to maintain reagent stability. Improper storage can lead to degradation and unreliable results.
2. Follow the instructions carefully when preparing and using the reagents. Deviations from the protocol can affect the accuracy of the assay.
3. Use appropriate protective equipment when handling the reagents. Some components may be hazardous if not handled correctly.
4. Ensure that all equipment used in the assay is properly calibrated. Inaccurate equipment can lead to erroneous results.
5. Dispose of used reagents and samples according to local regulations. Improper disposal can pose environmental and health risks.

Handling the HBDH Assay Kit with care is crucial for obtaining accurate results. Proper storage, adherence to protocols, and safe disposal practices are essential to ensure the kit's effectiveness and longevity.