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25-Hydroxy Vitamin D Assay Kit (Immunofluorescence Method)
The kit primarily consists of a detection card, buffer solution, and reagent cups, with quality control materials as optional components. The detection card is composed of a nitrocellulose membrane, glass fiber, absorbent paper, PVC board, and plastic casing. The buffer solution mainly contains 0.05mol/L Tris-HCl, with approximately 6g for 25 tests and 12g for 50 tests. The detection card includes VD antigen and goat anti-rabbit IgG polyclonal antibody. The reagent cups contain VD monoclonal antibody. The optional quality control material is a lyophilized powder primarily composed of VD antigen derived from cell culture.
Product English Name:
25-Hydroxy Vitamin D Assay Kit (Immunofluorescence Method)
Alias:
Vitamin D Test Kit,25-OH Vitamin D Kit
Intended Use:
This kit is designed for the quantitative detection of 25-hydroxy vitamin D levels in human serum, plasma, or whole blood. It is clinically used for the auxiliary diagnosis of diseases related to vitamin D deficiency.
Product Introduction

The 25-Hydroxy Vitamin D Assay Kit (Immunofluorescence Method) is a sophisticated diagnostic tool designed to measure the levels of 25-hydroxy vitamin D in human blood samples. This kit is essential for identifying vitamin D deficiency, which is crucial for maintaining bone health and overall well-being. The kit's components are meticulously designed to ensure accurate and reliable results, making it a trusted choice for clinical laboratories.

1. The kit features a highly sensitive detection card that utilizes immunofluorescence technology to provide precise measurements of 25-hydroxy vitamin D levels. This method ensures high specificity and sensitivity, reducing the likelihood of false positives or negatives.
2. The structural composition of the kit includes a detection card made of multiple layers, including a nitrocellulose membrane, glass fiber, absorbent paper, PVC board, and plastic casing. These materials work together to facilitate the efficient capture and detection of the target analyte.
3. The primary use of this kit is in clinical settings for the quantitative analysis of 25-hydroxy vitamin D in blood samples. It aids in the diagnosis and management of conditions related to vitamin D deficiency, such as osteoporosis, rickets, and other metabolic bone disorders.

The 25-Hydroxy Vitamin D Assay Kit, also known as the Vitamin D Test Kit, is a vital tool in modern clinical diagnostics. Its advanced immunofluorescence method and robust structural design make it a reliable choice for healthcare professionals. Whether you're diagnosing vitamin D deficiency or monitoring treatment efficacy, this kit offers the precision and accuracy needed for optimal patient care.

Main Features

The 25-Hydroxy Vitamin D Assay Kit offers a range of features designed to enhance diagnostic accuracy and efficiency. Its immunofluorescence-based detection method ensures high sensitivity and specificity, while its user-friendly design simplifies the testing process.

1. The kit employs immunofluorescence technology, which provides highly accurate and reliable results. This method is particularly effective in detecting low concentrations of 25-hydroxy vitamin D, making it ideal for diagnosing early-stage deficiencies.
2. The detection card is designed with multiple layers, including a nitrocellulose membrane and glass fiber, which enhance the capture and detection of the target analyte. This structural design ensures consistent performance across different sample types.
3. The buffer solution is formulated to maintain optimal pH and ionic strength, ensuring that the immunofluorescence reaction proceeds efficiently. This contributes to the overall accuracy and reliability of the test results.
4. The kit includes reagent cups containing VD monoclonal antibody, which specifically binds to 25-hydroxy vitamin D. This specificity reduces the risk of cross-reactivity with other substances, further enhancing the test's accuracy.
5. The optional quality control material allows laboratories to verify the performance of the kit, ensuring that it meets the required standards for diagnostic testing. This feature is particularly important for maintaining the integrity of clinical results.

The 25-Hydroxy Vitamin D Assay Kit stands out for its advanced immunofluorescence technology and robust design. Its features, such as the multi-layered detection card and specialized buffer solution, ensure high accuracy and reliability. Whether you're conducting routine screenings or detailed diagnostic tests, this kit provides the tools needed for precise and efficient vitamin D analysis.

Clinical Applications

The 25-Hydroxy Vitamin D Assay Kit is widely used in clinical settings for the diagnosis and management of vitamin D deficiency-related conditions. Its accurate and reliable results make it an essential tool for healthcare professionals.

1. The kit is commonly used in the diagnosis of osteoporosis, a condition characterized by weakened bones due to vitamin D deficiency. Accurate measurement of 25-hydroxy vitamin D levels helps in assessing bone health and guiding treatment plans.
2. In pediatric care, the kit is used to diagnose rickets, a disease caused by severe vitamin D deficiency. Early detection and treatment are crucial for preventing long-term bone deformities and other complications.
3. The kit is also employed in the management of chronic kidney disease, where vitamin D deficiency is a common complication. Regular monitoring of vitamin D levels helps in adjusting treatment regimens to improve patient outcomes.
4. In endocrinology, the kit is used to assess vitamin D status in patients with metabolic bone disorders. Accurate measurement of 25-hydroxy vitamin D levels is essential for diagnosing and managing these conditions.
5. The kit is also used in research settings to study the role of vitamin D in various diseases. Its high sensitivity and specificity make it a valuable tool for investigating the impact of vitamin D on health and disease.

The 25-Hydroxy Vitamin D Assay Kit is a versatile tool in clinical practice, used for diagnosing and managing a range of vitamin D deficiency-related conditions. Its applications span from osteoporosis and rickets to chronic kidney disease and metabolic bone disorders. With its accurate and reliable results, this kit is an indispensable resource for healthcare professionals.

Purchasing Guide

When purchasing the 25-Hydroxy Vitamin D Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product specifications: Ensure that the kit meets your laboratory's requirements in terms of sensitivity, specificity, and detection range. Check the number of tests per kit and the shelf life to ensure it aligns with your usage needs.
2. Product types: The kit is available in different formats, including 25-test and 50-test versions. Choose the type that best suits your laboratory's volume and frequency of testing.
3. Budget: Consider the cost per test and the overall budget for your laboratory. While higher-priced kits may offer additional features, ensure that they provide value for money based on your specific needs.
4. Usage scenarios: Evaluate the intended use of the kit, whether for routine screening, diagnostic testing, or research purposes. Ensure that the kit's features and performance align with your clinical or research objectives.

Purchasing the 25-Hydroxy Vitamin D Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can select a kit that meets your laboratory's needs and ensures accurate and reliable results. This guide aims to simplify the decision-making process, helping you choose the right kit for your clinical or research applications.

 Precautions

Proper handling and usage of the 25-Hydroxy Vitamin D Assay Kit are essential to ensure accurate results and maintain the integrity of the components. This section outlines key precautions to follow during storage, handling, and testing.

1. Storage: Store the kit at the recommended temperature to preserve the stability of the components. Avoid exposure to extreme temperatures or direct sunlight, which can degrade the reagents.
2. Handling: Use gloves and other protective equipment when handling the kit components to prevent contamination. Follow the manufacturer's instructions for proper handling and disposal of reagents.
3. Testing: Ensure that the testing environment is clean and free from contaminants. Follow the recommended protocol for sample preparation and testing to avoid errors and ensure accurate results.
4. Quality control: Regularly use the optional quality control material to verify the performance of the kit. This helps in identifying any issues with the kit's components or the testing process.
5. Disposal: Dispose of used components according to local regulations and guidelines. Proper disposal is essential to prevent environmental contamination and ensure safety.

The 25-Hydroxy Vitamin D Assay Kit requires careful handling and storage to maintain its performance and ensure accurate results. By following the recommended precautions, you can minimize the risk of contamination, errors, and component degradation. Proper usage and disposal of the kit components are essential for maintaining the integrity of your laboratory's testing process and ensuring patient safety.

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