The α-Hydroxybutyrate Dehydrogenase Assay Kit is a critical tool in clinical diagnostics, enabling the precise measurement of HBDH activity in serum. This enzyme is a marker for tissue damage, particularly in the heart and liver. The kit utilizes the UV kinetic method recommended by the International Federation of Clinical Chemistry (IFCC), ensuring high accuracy and reliability. It is composed of two reagents that work synergistically to provide consistent and reproducible results.

1. The kit is designed with a focus on precision and ease of use. It employs a UV kinetic method, which is highly sensitive and specific for measuring HBDH activity. This method ensures minimal interference from other serum components, providing reliable results.
2. The composition of the kit includes Reagent 1 (R1) and Reagent 2 (R2). R1 contains tris buffer and α-ketobutyrate, which are essential for the enzymatic reaction. R2 contains β-NADH, a coenzyme that plays a crucial role in the detection process. Together, these reagents create an optimal environment for accurate HBDH measurement.
3. The primary use of this kit is to determine HBDH activity in human serum, which is a key indicator of cardiac and liver health. Elevated levels of HBDH can signal tissue damage, making this kit an invaluable tool for diagnosing and monitoring various medical conditions.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a reliable and precise tool for measuring HBDH activity in serum. Its composition, featuring tris buffer, α-ketobutyrate, and β-NADH, ensures accurate results. This kit is essential for assessing cardiac and liver function, making it a staple in clinical diagnostics.

The α-Hydroxybutyrate Dehydrogenase Assay Kit offers high sensitivity and specificity in measuring HBDH activity. Its UV kinetic method ensures accurate and reproducible results, making it a trusted choice for clinical laboratories.

1. The kit utilizes the UV kinetic method, which is highly sensitive and specific for detecting HBDH activity. This method minimizes interference from other serum components, ensuring reliable results.
2. The two-reagent system (R1 and R2) is designed for optimal performance. R1 contains tris buffer and α-ketobutyrate, while R2 contains β-NADH. Together, they create an ideal environment for the enzymatic reaction.
3. The kit is easy to use, with a straightforward protocol that ensures consistent results. It is suitable for both manual and automated systems, making it versatile for various laboratory settings.
4. The kit provides a wide dynamic range, allowing for the accurate measurement of HBDH activity across a broad spectrum of concentrations. This feature is particularly useful in clinical diagnostics.
5. The kit is stable and has a long shelf life, ensuring that it remains effective over time. This stability is crucial for maintaining the accuracy and reliability of test results.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its precision, ease of use, and reliability. Its UV kinetic method and two-reagent system ensure accurate HBDH measurement, making it an indispensable tool in clinical diagnostics.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is widely used in clinical settings to assess cardiac and liver function. It is particularly valuable in diagnosing and monitoring conditions such as myocardial infarction and liver disease.

1. The kit is commonly used in the diagnosis of myocardial infarction. Elevated levels of HBDH in serum can indicate heart muscle damage, making this kit a critical tool in cardiology.
2. It is also used to assess liver function. Elevated HBDH levels can signal liver damage or disease, providing valuable information for hepatologists.
3. The kit is employed in monitoring the progression of certain diseases. Regular measurement of HBDH activity can help track the effectiveness of treatments and the progression of conditions.
4. It is used in research settings to study the role of HBDH in various physiological and pathological processes. This research can lead to new insights into disease mechanisms and potential treatments.
5. The kit is also used in routine health check-ups to assess overall tissue health. Elevated HBDH levels can be an early indicator of tissue damage, prompting further investigation.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, particularly in cardiology and hepatology. Its ability to accurately measure HBDH activity makes it invaluable for diagnosing and monitoring various medical conditions.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product specifications: Ensure the kit meets the required sensitivity, specificity, and dynamic range for your laboratory's applications. Check the shelf life and storage conditions to ensure long-term usability.
2. Product types: There may be different versions of the kit available, such as manual or automated versions. Choose the one that best fits your laboratory's workflow and equipment.
3. Budget: Consider the cost of the kit, including any additional reagents or equipment that may be required. Ensure that the kit provides good value for money in terms of performance and reliability.
4. Usage scenarios: Evaluate the intended use of the kit, whether for routine diagnostics, research, or specialized applications. Ensure that the kit is suitable for your specific needs.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable results.

Proper handling and storage of the α-Hydroxybutyrate Dehydrogenase Assay Kit are essential to ensure accurate results and maintain the integrity of the reagents.

1. Storage: Store the kit at the recommended temperature to maintain reagent stability. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Use aseptic techniques when handling the reagents to prevent contamination. Ensure that all equipment used is clean and free from residues.
3. Expiration: Check the expiration date before use. Do not use expired reagents, as they may yield inaccurate results.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure accurate measurements. Follow the manufacturer's guidelines for calibration procedures.
5. Disposal: Dispose of used reagents and materials according to local regulations. Ensure that hazardous materials are handled and disposed of safely.

The α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful handling and storage to ensure accurate results. By following proper storage, handling, and disposal procedures, you can maintain the integrity of the reagents and achieve reliable outcomes.