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Alpha-Amylase (AMY) Assay Kit (IFCC Recommended Method)
The kit is a liquid dual-reagent system, consisting of Reagent 1 (R1) and Reagent 2 (R2). R1 contains HEPES (50 mmol/L), α-glucosidase (8 KU/L), sodium chloride (70 mmol/L), and calcium chloride (1 mmol/L). R2 contains 4,6-ethylidene-4-nitrophenol-α-heptoside (E-PNP-G7) at a concentration of 5 mmol/L. This composition ensures accurate and reliable measurement of α-amylase activity in serum samples.
Product English Name:
Alpha-Amylase (AMY) Assay Kit (IFCC Recommended Method)
Alias:
AMY Test Kit,Alpha-Amylase Detection Kit
Intended Use:
This kit is designed for the in vitro determination of α-amylase (AMY) levels in human serum. It is widely used in clinical laboratories to assess pancreatic function and diagnose conditions such as pancreatitis, salivary gland disorders, and other diseases related to abnormal α-amylase activity.
Product Introduction

The Alpha-Amylase (AMY) Assay Kit is a high-quality diagnostic tool based on the IFCC recommended method. It provides precise and reproducible results for measuring α-amylase activity in serum. The kit is essential for clinical diagnostics, offering a reliable solution for laboratories to evaluate pancreatic and salivary gland health.

1. The kit features a dual-reagent system that ensures stability and accuracy. The inclusion of α-glucosidase in R1 enhances specificity, while E-PNP-G7 in R2 acts as a substrate for α-amylase, enabling precise enzymatic activity measurement.
2. The composition includes HEPES buffer, which maintains optimal pH conditions, and calcium chloride, which stabilizes the enzyme. Sodium chloride ensures ionic strength, while α-glucosidase and E-PNP-G7 work together to provide a robust assay system.
3. This kit is primarily used to measure α-amylase levels in human serum, aiding in the diagnosis of pancreatic and salivary gland disorders. Its reliability and ease of use make it a preferred choice in clinical laboratories.

The Alpha-Amylase (AMY) Assay Kit, also known as the AMY Test Kit, is a vital tool in clinical diagnostics. Its dual-reagent system and carefully balanced composition ensure accurate and reliable results, making it indispensable for assessing α-amylase activity in serum.

Main Features

The Alpha-Amylase (AMY) Assay Kit offers high precision, stability, and ease of use. Its dual-reagent system and optimized composition ensure reliable measurement of α-amylase activity in serum samples.

1. The kit employs a dual-reagent system, enhancing stability and accuracy. R1 and R2 work synergistically to provide consistent and reproducible results.
2. The inclusion of α-glucosidase in R1 ensures high specificity for α-amylase, minimizing interference from other enzymes.
3. E-PNP-G7 in R2 acts as a substrate, enabling precise measurement of enzymatic activity through a colorimetric reaction.
4. The HEPES buffer maintains optimal pH conditions, while calcium chloride stabilizes the enzyme, ensuring reliable performance.
5. The kit is designed for high-throughput laboratories, offering simplicity and efficiency in measuring α-amylase levels.

The Alpha-Amylase (AMY) Assay Kit stands out for its precision, stability, and ease of use. Its dual-reagent system and optimized composition make it a reliable choice for clinical laboratories, ensuring accurate measurement of α-amylase activity.

Clinical Applications

This kit is widely used in clinical settings to diagnose pancreatic and salivary gland disorders. It provides reliable measurement of α-amylase activity, aiding in the assessment of pancreatic function and disease diagnosis.

1. The kit is used to diagnose acute and chronic pancreatitis by measuring elevated α-amylase levels in serum.
2. It aids in the diagnosis of salivary gland disorders, such as sialadenitis, by detecting abnormal α-amylase activity.
3. The kit is employed in monitoring patients with pancreatic cancer, providing insights into disease progression and treatment efficacy.
4. It is used in routine health check-ups to assess pancreatic function and detect early signs of pancreatic disorders.
5. The kit is also applied in research studies to investigate the role of α-amylase in various physiological and pathological conditions.

The Alpha-Amylase (AMY) Assay Kit is a versatile tool in clinical diagnostics, widely used for diagnosing pancreatic and salivary gland disorders. Its reliability and precision make it indispensable in both clinical and research settings.

Purchasing Guide

When purchasing the Alpha-Amylase (AMY) Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Evaluate the product specifications, including reagent composition, stability, and shelf life, to ensure compatibility with your laboratory's requirements.
2. Choose between different kit types, such as single-reagent or dual-reagent systems, based on your laboratory's workflow and throughput needs.
3. Consider your budget and compare prices from different suppliers to find a cost-effective solution without compromising quality.
4. Assess the intended use, such as clinical diagnostics or research, to select a kit that aligns with your specific application.

Purchasing the Alpha-Amylase (AMY) Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure the kit meets your laboratory's needs and delivers reliable results.

 Precautions

Proper handling and storage of the Alpha-Amylase (AMY) Assay Kit are essential to ensure accurate results and maintain reagent stability.

1. Store the kit at the recommended temperature (2-8°C) to maintain reagent stability and prevent degradation.
2. Avoid repeated freeze-thaw cycles, as this can compromise reagent performance and lead to inaccurate results.
3. Follow the manufacturer's instructions for reagent preparation and handling to ensure consistency and reliability.
4. Use appropriate protective equipment, such as gloves and lab coats, when handling reagents to prevent contamination and ensure safety.
5. Regularly calibrate and maintain laboratory equipment, such as spectrophotometers, to ensure accurate measurement of α-amylase activity.

Handling the Alpha-Amylase (AMY) Assay Kit with care is crucial for obtaining accurate results. Proper storage, handling, and equipment maintenance are essential to ensure the kit's reliability and performance in clinical diagnostics.

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