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α-Amylase Assay Kit (EPS-G7 Method)
The α-Amylase Assay Kit (EPS-G7 Method) consists of two main reagents: Reagent 1 (R1) and Reagent 2 (R2). Reagent 1 contains Good's buffer (100 mmol/L), sodium chloride (50 mmol/L), magnesium chloride (10 mmol/L), and α-glucosidase (≥2000 U/L). Reagent 2 includes Good's buffer (100 mmol/L) and 4,6-ethylidene-p-nitrophenyl-α-D-maltoheptaoside (EPS-PNP-G7) (1.6 mmol/L). These components work together to provide a precise and reliable method for measuring α-amylase activity in human serum.
Product English Name:
α-Amylase Assay Kit (EPS-G7 Method)
Alias:
Amylase Test Kit,EPS-G7 Amylase Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of α-amylase (α-AMY) activity in human serum. It is widely used in clinical laboratories to assess pancreatic function and diagnose conditions related to abnormal amylase levels, such as pancreatitis and other pancreatic disorders.
Product Introduction

The α-Amylase Assay Kit (EPS-G7 Method) is a high-quality diagnostic tool used to measure the activity of α-amylase in human serum. This enzyme plays a crucial role in carbohydrate metabolism, and its levels can indicate various health conditions. The kit employs a colorimetric method based on the EPS-G7 substrate, which provides accurate and reproducible results. It is suitable for use in clinical laboratories and research settings, offering a reliable solution for diagnosing and monitoring pancreatic diseases.

1. The kit features a highly sensitive and specific assay method, utilizing the EPS-G7 substrate to ensure precise measurement of α-amylase activity. The reaction is optimized to minimize interference from other serum components, providing reliable results even in complex biological samples.
2. The composition of the kit includes two reagents: Reagent 1 and Reagent 2. Reagent 1 contains a buffer system, salts, and α-glucosidase, while Reagent 2 includes the EPS-G7 substrate. These components are carefully formulated to ensure stability and consistency in the assay performance.
3. The primary use of this kit is to quantitatively determine α-amylase activity in human serum. It is particularly useful in diagnosing pancreatic disorders, monitoring disease progression, and evaluating the effectiveness of treatment. The kit is also used in research to study the role of α-amylase in various physiological and pathological processes.

The α-Amylase Assay Kit (EPS-G7 Method) is a reliable and efficient tool for measuring α-amylase activity in human serum. Its precise composition and optimized assay method make it a valuable resource for clinical diagnostics and research. Whether you're diagnosing pancreatic disorders or studying enzyme activity, this kit offers the accuracy and consistency you need.

Main Features

The α-Amylase Assay Kit (EPS-G7 Method) offers a range of features designed to ensure accurate and reliable measurement of α-amylase activity. Its key features include high sensitivity, specificity, and ease of use, making it an ideal choice for clinical laboratories and research settings.

1. High Sensitivity: The kit is designed to detect even low levels of α-amylase activity, ensuring accurate results in a wide range of clinical samples.
2. Specificity: The EPS-G7 substrate is highly specific for α-amylase, minimizing interference from other enzymes and serum components.
3. Stability: The reagents are formulated to maintain stability over time, ensuring consistent performance throughout the shelf life of the kit.
4. Ease of Use: The kit includes clear instructions and is designed for straightforward use, reducing the risk of user error and ensuring reliable results.
5. Reproducibility: The assay method is optimized to provide reproducible results, making it suitable for both routine clinical use and research applications.

The α-Amylase Assay Kit (EPS-G7 Method) stands out for its high sensitivity, specificity, and ease of use. Whether you're working in a clinical lab or conducting research, this kit offers the reliability and consistency you need to achieve accurate results. Its optimized assay method and stable reagents make it a trusted choice for measuring α-amylase activity.

Clinical Applications

The α-Amylase Assay Kit (EPS-G7 Method) is widely used in clinical settings to diagnose and monitor pancreatic disorders. Its accurate and reliable measurement of α-amylase activity makes it an essential tool for assessing pancreatic function and detecting abnormalities.

1. Diagnosis of Pancreatitis: The kit is commonly used to diagnose acute and chronic pancreatitis by measuring elevated levels of α-amylase in serum.
2. Monitoring Disease Progression: Clinicians use the kit to monitor the progression of pancreatic diseases and evaluate the effectiveness of treatment.
3. Research Applications: Researchers use the kit to study the role of α-amylase in various physiological and pathological processes, including carbohydrate metabolism and digestive disorders.
4. Screening for Pancreatic Disorders: The kit is also used in routine health screenings to detect early signs of pancreatic dysfunction.
5. Evaluation of Treatment Efficacy: The kit helps clinicians assess the effectiveness of therapeutic interventions aimed at managing pancreatic diseases.

The α-Amylase Assay Kit (EPS-G7 Method) is a versatile tool with a wide range of clinical applications. From diagnosing pancreatitis to monitoring disease progression, this kit provides the accuracy and reliability needed for effective patient care. Its use in research further underscores its value in advancing our understanding of pancreatic function and disease.

Purchasing Guide

When purchasing the α-Amylase Assay Kit (EPS-G7 Method), it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product Specifications: Ensure that the kit meets your laboratory's requirements in terms of sensitivity, specificity, and stability. Check the shelf life and storage conditions to ensure optimal performance.
2. Product Types: The kit is available in different formats, including single-use and bulk packages. Choose the format that best suits your laboratory's workflow and sample volume.
3. Budget Considerations: Evaluate the cost of the kit in relation to your laboratory's budget. Consider the long-term value and reliability of the product when making your decision.
4. Intended Use: Determine the primary application of the kit, whether for routine clinical use, research, or both. Ensure that the kit is suitable for your specific needs and intended use cases.

Purchasing the α-Amylase Assay Kit (EPS-G7 Method) requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can select a kit that meets your laboratory's needs and ensures accurate and reliable results. Whether for clinical diagnostics or research, this kit offers the quality and performance you need.

 Precautions

When using the α-Amylase Assay Kit (EPS-G7 Method), it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to maintain reagent stability. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling Precautions: Use aseptic techniques when handling reagents to prevent contamination. Wear appropriate personal protective equipment (PPE) to ensure safety.
3. Reagent Preparation: Follow the instructions carefully when preparing reagents. Ensure that all components are mixed thoroughly and used within the specified time frame.
4. Sample Handling: Use fresh serum samples for accurate results. Avoid using hemolyzed or lipemic samples, as they may interfere with the assay.
5. Quality Control: Perform regular quality control checks to ensure the accuracy and reliability of the assay. Use control materials provided with the kit or from an external source.

Proper handling and storage of the α-Amylase Assay Kit (EPS-G7 Method) are essential for obtaining accurate and reliable results. By following the recommended precautions, you can ensure the integrity of the reagents and maintain the performance of the kit. Whether you're a clinician or a researcher, these precautions will help you achieve the best possible outcomes.

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