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α-Amylase Assay Kit (EPS-G7 Method)
The α-Amylase Assay Kit (EPS-G7 Method) consists of two main components: R1 and R2. R1 contains 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) at a concentration of 50mmol/L and α-glucosidase at a concentration of ≥2KU/L. R2 contains ethylidene-4-NP-maltoheptaoside (EPS) at a concentration of 3mmol/L. These components work together to provide a reliable and accurate method for measuring α-amylase activity in human serum.
Product English Name:
α-Amylase Assay Kit (EPS-G7 Method)
Alias:
Amylase Test Kit,EPS-G7 Amylase Kit
Intended Use:
This kit is specifically designed for use in clinical chemistry laboratories to measure the activity of α-amylase (α-AMY) in human serum. It is an essential tool for diagnosing and monitoring conditions related to pancreatic and salivary gland function, as well as other disorders where α-amylase levels are indicative of disease states.
Product Introduction

The α-Amylase Assay Kit (EPS-G7 Method) is a highly specialized diagnostic tool used in clinical laboratories to measure the activity of α-amylase in human serum. This enzyme is crucial for the digestion of carbohydrates, and its levels can provide important diagnostic information about pancreatic and salivary gland function. The kit utilizes a unique EPS-G7 method, which offers high sensitivity and specificity, making it a preferred choice for clinical diagnostics.

1. The α-Amylase Assay Kit is known for its high sensitivity and specificity, ensuring accurate measurement of α-amylase activity. The EPS-G7 method used in this kit is particularly effective in minimizing interference from other enzymes, providing reliable results even in complex biological samples.
2. The kit is composed of two main reagents: R1 and R2. R1 contains HEPES buffer and α-glucosidase, which are essential for the enzymatic reaction. R2 contains EPS, a substrate that is specifically cleaved by α-amylase, producing a measurable signal that correlates with enzyme activity.
3. This kit is primarily used in clinical chemistry laboratories to assess α-amylase levels in human serum. It is particularly useful in diagnosing pancreatic disorders, such as pancreatitis, and in monitoring the effectiveness of treatment. Additionally, it can be used in research settings to study the role of α-amylase in various physiological and pathological processes.

The α-Amylase Assay Kit (EPS-G7 Method) is a reliable and accurate tool for measuring α-amylase activity in human serum. Its high sensitivity and specificity, combined with its unique EPS-G7 method, make it an essential diagnostic tool in clinical laboratories. Whether you're diagnosing pancreatic disorders or conducting research on enzyme activity, this kit provides the precision and reliability you need.

Main Features

The α-Amylase Assay Kit (EPS-G7 Method) offers several key features that make it a preferred choice for clinical diagnostics. These include high sensitivity, specificity, and a unique EPS-G7 method that minimizes interference from other enzymes.

1. High Sensitivity: The kit is designed to detect even low levels of α-amylase activity, making it suitable for diagnosing early-stage pancreatic disorders.
2. Specificity: The EPS-G7 method ensures that the measurement is specific to α-amylase, reducing the risk of false positives due to interference from other enzymes.
3. Reliable Results: The kit provides consistent and reproducible results, which are crucial for accurate diagnosis and monitoring of disease states.
4. Ease of Use: The kit is user-friendly, with clear instructions and a straightforward protocol that can be easily followed by laboratory personnel.
5. Versatility: In addition to clinical diagnostics, the kit can also be used in research settings to study the role of α-amylase in various physiological processes.

The α-Amylase Assay Kit (EPS-G7 Method) is a versatile and reliable tool for measuring α-amylase activity. Its high sensitivity and specificity, combined with its ease of use, make it an essential tool for both clinical diagnostics and research. Whether you're diagnosing pancreatic disorders or studying enzyme activity, this kit provides the accuracy and reliability you need.

Clinical Applications

The α-Amylase Assay Kit (EPS-G7 Method) is widely used in clinical settings for the diagnosis and monitoring of pancreatic and salivary gland disorders. It is also used in research to study the role of α-amylase in various physiological processes.

1. Diagnosis of Pancreatic Disorders: The kit is commonly used to diagnose acute and chronic pancreatitis, as well as other pancreatic disorders. Elevated levels of α-amylase in serum are a key indicator of these conditions.
2. Monitoring Treatment: The kit is used to monitor the effectiveness of treatment for pancreatic disorders. Regular measurement of α-amylase levels can help healthcare providers adjust treatment plans as needed.
3. Salivary Gland Disorders: The kit can also be used to diagnose disorders of the salivary glands, such as sialadenitis, where α-amylase levels may be elevated.
4. Research Applications: In addition to clinical diagnostics, the kit is used in research to study the role of α-amylase in various physiological processes, including carbohydrate metabolism and digestion.
5. Quality Control: The kit is also used in quality control processes in clinical laboratories to ensure the accuracy and reliability of α-amylase measurements.

The α-Amylase Assay Kit (EPS-G7 Method) is a versatile tool with a wide range of clinical and research applications. Its ability to accurately measure α-amylase levels makes it an essential tool for diagnosing and monitoring pancreatic and salivary gland disorders, as well as for conducting research on enzyme activity.

Purchasing Guide

When purchasing the α-Amylase Assay Kit (EPS-G7 Method), it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides an overview of key considerations to help you make an informed decision.

1. Product Specifications: Ensure that the kit meets your laboratory's specific requirements, including sensitivity, specificity, and compatibility with your existing equipment.
2. Product Types: Consider the different types of kits available, such as those designed for high-throughput screening or for use in research settings. Choose the one that best fits your needs.
3. Budget: Determine your budget and compare prices from different suppliers. Keep in mind that higher-priced kits may offer additional features or higher quality components.
4. Intended Use: Consider the intended use of the kit, whether for clinical diagnostics, research, or quality control. Ensure that the kit you choose is suitable for your specific application.

Purchasing the α-Amylase Assay Kit (EPS-G7 Method) requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you select the right kit for your laboratory's needs.

 Precautions

When using the α-Amylase Assay Kit (EPS-G7 Method), it is important to follow certain precautions to ensure accurate results and safe handling. This section provides an overview of key precautions to keep in mind.

1. Proper Storage: Store the kit at the recommended temperature to maintain the stability and effectiveness of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling Reagents: Handle the reagents with care, using appropriate personal protective equipment (PPE) such as gloves and lab coats. Avoid contact with skin or eyes.
3. Calibration: Ensure that all equipment used with the kit, such as spectrophotometers and pipettes, is properly calibrated to ensure accurate measurements.
4. Sample Handling: Handle serum samples with care to avoid contamination. Use sterile techniques when collecting and processing samples.
5. Disposal: Dispose of used reagents and samples according to local regulations and laboratory protocols. Avoid improper disposal that could harm the environment.

Using the α-Amylase Assay Kit (EPS-G7 Method) requires careful attention to storage, handling, calibration, sample handling, and disposal. By following these precautions, you can ensure accurate results and safe handling of the kit and its components.

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