The α-Hydroxybutyrate Dehydrogenase Assay Kit (α-HBDH) is a specialized diagnostic tool designed to measure the activity of α-HBDH in biological samples. Utilizing the UV kinetic method, this kit provides precise and reliable results, making it essential for clinical diagnostics, particularly in assessing myocardial health. The kit includes a carefully formulated buffer and starter solution to ensure optimal reaction conditions and accurate enzyme activity measurement.

1. The α-HBDH assay kit is characterized by its high sensitivity and specificity, enabling accurate detection of α-HBDH activity even in low-concentration samples. The UV kinetic method ensures rapid and reliable results, making it suitable for high-throughput clinical laboratories.
2. The kit is composed of two main components: buffer R1 and starter R2. Buffer R1 contains Tris buffer, α-ketobutyrate, and sodium azide, while starter R2 includes NADH and stabilizers. This combination ensures stable and consistent enzyme activity measurement.
3. The primary use of the α-HBDH assay kit is to measure LDH-1 activity in serum, which is crucial for diagnosing myocardial infarction and other cardiac conditions. It is also used in conjunction with other enzymes like LDH, GOT, CK, and CK-MB to create a comprehensive myocardial enzyme profile.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Test Kit, is a vital tool in clinical diagnostics. Its high sensitivity and specificity make it indispensable for measuring LDH-1 activity in serum, particularly in the context of myocardial health. The kit's well-balanced composition ensures accurate and reliable results, making it a trusted choice for healthcare professionals.

The α-HBDH assay kit offers high sensitivity, specificity, and reliability in measuring α-HBDH activity. Its UV kinetic method ensures rapid and accurate results, making it ideal for clinical diagnostics.

1. High Sensitivity: The kit can detect low levels of α-HBDH activity, making it suitable for early diagnosis of myocardial infarction and other cardiac conditions.
2. Specificity: The kit specifically measures LDH-1 activity, providing accurate results even in the presence of other LDH isoenzymes.
3. Rapid Results: The UV kinetic method allows for quick measurement of enzyme activity, reducing turnaround time in clinical laboratories.
4. Stability: The inclusion of stabilizers in the starter solution ensures long-term stability of the reagents, maintaining consistent performance over time.
5. Comprehensive Analysis: The kit is part of a broader myocardial enzyme profile, allowing for a more comprehensive assessment of cardiac health.

The α-HBDH assay kit stands out for its high sensitivity, specificity, and rapid results. Its ability to accurately measure LDH-1 activity makes it an essential tool in diagnosing myocardial infarction and other cardiac conditions. The kit's stability and comprehensive analysis capabilities further enhance its value in clinical diagnostics.

The α-HBDH assay kit is widely used in clinical settings to diagnose myocardial infarction and other cardiac conditions. It is also used in research to study enzyme activity in various tissues.

1. Myocardial Infarction Diagnosis: The kit is used to measure LDH-1 activity in serum, which is elevated in patients with myocardial infarction.
2. Cardiac Health Monitoring: Regular measurement of α-HBDH activity helps monitor cardiac health and detect early signs of heart disease.
3. Research Applications: The kit is used in research to study the activity of α-HBDH in different tissues, providing insights into enzyme function and regulation.
4. Clinical Laboratories: The kit is a standard tool in clinical laboratories for assessing myocardial enzyme profiles and diagnosing cardiac conditions.
5. Comparative Studies: The kit is used in comparative studies to evaluate the effectiveness of different diagnostic methods for cardiac conditions.

The α-HBDH assay kit is a versatile tool in both clinical and research settings. Its primary application is in diagnosing myocardial infarction and monitoring cardiac health. The kit's ability to provide accurate and reliable results makes it a valuable asset in clinical laboratories and research studies.

When purchasing the α-HBDH assay kit, consider factors such as product specifications, types, budget, and intended use to ensure the best fit for your needs.

1. Product Specifications: Ensure the kit meets your laboratory's requirements for sensitivity, specificity, and stability.
2. Product Types: Choose between different kit formats, such as single-use or bulk packs, based on your laboratory's workflow and volume needs.
3. Budget: Consider the cost of the kit and any additional reagents or equipment required for its use.
4. Intended Use: Select a kit that is specifically designed for your intended application, whether it's clinical diagnostics or research.

Purchasing the α-HBDH assay kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides accurate and reliable results.

Proper handling and storage of the α-HBDH assay kit are essential to ensure accurate results and maintain reagent stability.

1. Storage Conditions: Store the kit at the recommended temperature to maintain reagent stability and performance.
2. Handling: Follow proper handling procedures to avoid contamination and ensure accurate results.
3. Expiration Date: Check the expiration date before use and do not use expired reagents.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure accurate measurements.
5. Disposal: Dispose of used reagents and materials according to local regulations to prevent environmental contamination.

Proper handling, storage, and disposal of the α-HBDH assay kit are crucial for maintaining reagent stability and ensuring accurate results. By following these precautions, you can maximize the kit's performance and reliability in clinical and research settings.