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α-Hydroxybutyrate Dehydrogenase Assay Kit (Ketobutyrate Substrate Method)
The α-Hydroxybutyrate Dehydrogenase Assay Kit is composed of two main reagents: Reagent 1 (R1) contains a phosphate buffer at 50mmol/L and α-ketobutyrate at 4.5mmol/L, while Reagent 2 (R2) includes NADH at 1.1mmol/L. It is crucial to note that reagents from different batches should not be mixed to ensure accurate results.
Product English Name:
α-Hydroxybutyrate Dehydrogenase Assay Kit (Ketobutyrate Substrate Method)
Alias:
HBDH Assay Kit,Ketobutyrate Method Kit
Intended Use:
This kit is specifically designed for the quantitative determination of α-hydroxybutyrate dehydrogenase (HBDH) activity in serum, which is a key enzyme in the metabolism of ketone bodies. It is widely used in clinical laboratories to assess liver function and diagnose liver diseases.
Product Introduction

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a vital tool in clinical diagnostics, particularly for evaluating liver health. It utilizes a ketobutyrate substrate method to measure HBDH activity, providing critical insights into metabolic processes and liver function. The kit is designed for ease of use, ensuring reliable and reproducible results in clinical settings.

1. The kit features a highly sensitive and specific assay method, ensuring accurate measurement of HBDH activity. It is optimized for use in automated analyzers, making it suitable for high-throughput clinical laboratories.
2. The composition includes two reagents: R1, which contains a phosphate buffer and α-ketobutyrate, and R2, which includes NADH. These components work synergistically to facilitate the enzymatic reaction necessary for HBDH activity measurement.
3. The primary use of this kit is in the diagnosis and monitoring of liver diseases. By measuring HBDH activity, clinicians can assess liver function and detect abnormalities early, aiding in timely intervention and treatment.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is an essential diagnostic tool in clinical laboratories. Its precise measurement of HBDH activity provides valuable insights into liver health, making it indispensable for diagnosing and managing liver diseases.

Main Features

The α-Hydroxybutyrate Dehydrogenase Assay Kit offers a reliable and accurate method for measuring HBDH activity, crucial for assessing liver function and diagnosing liver diseases.

1. The kit employs a ketobutyrate substrate method, ensuring high specificity and sensitivity in measuring HBDH activity.
2. It is compatible with automated analyzers, facilitating high-throughput testing in clinical laboratories.
3. The kit includes two reagents: R1 and R2, which are optimized for precise enzymatic reactions.
4. It provides consistent and reproducible results, essential for accurate clinical diagnostics.
5. The kit is designed for ease of use, with clear instructions and minimal preparation required.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its accuracy, reliability, and ease of use. Its ability to measure HBDH activity with precision makes it a valuable tool in clinical diagnostics, particularly for liver function assessment.

Clinical Applications

The α-Hydroxybutyrate Dehydrogenase Assay Kit is widely used in clinical settings for diagnosing and monitoring liver diseases, providing critical insights into liver function.

1. The kit is used in the diagnosis of liver diseases, such as hepatitis and cirrhosis, by measuring HBDH activity.
2. It aids in monitoring liver function in patients undergoing treatment for liver-related conditions.
3. The kit is employed in research studies to investigate the role of HBDH in metabolic processes.
4. It is used in clinical trials to evaluate the efficacy of new liver disease treatments.
5. The kit is also utilized in routine health check-ups to assess liver health and detect early signs of liver dysfunction.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical practice, offering valuable insights into liver health. Its applications range from disease diagnosis to treatment monitoring, making it an indispensable asset in healthcare.

Purchasing Guide

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure optimal performance and cost-effectiveness.

1. Product specifications: Ensure the kit meets the required sensitivity and specificity for your clinical laboratory's needs.
2. Product types: Choose between different kit formats, such as manual or automated, based on your laboratory's workflow.
3. Budget: Consider the cost per test and overall budget constraints when selecting the kit.
4. Use scenarios: Evaluate the kit's compatibility with your laboratory's equipment and testing volume to ensure seamless integration.

Selecting the right α-Hydroxybutyrate Dehydrogenase Assay Kit involves careful consideration of various factors. By aligning the kit's features with your laboratory's needs, you can ensure accurate and efficient diagnostic testing.

 Precautions

Proper handling and storage of the α-Hydroxybutyrate Dehydrogenase Assay Kit are essential to maintain its performance and ensure accurate results.

1. Store the kit at the recommended temperature to preserve reagent stability and prevent degradation.
2. Avoid mixing reagents from different batches, as this can lead to inconsistent results.
3. Follow the manufacturer's instructions for reagent preparation and assay procedure to ensure accuracy.
4. Use appropriate protective equipment when handling reagents to prevent contamination and ensure safety.
5. Regularly calibrate and maintain laboratory equipment to ensure consistent performance and reliable results.

Adhering to the recommended precautions when using the α-Hydroxybutyrate Dehydrogenase Assay Kit is crucial for maintaining its efficacy. Proper storage, handling, and adherence to protocols ensure accurate and reliable diagnostic results.

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