The α-Hydroxybutyrate Dehydrogenase Assay Kit (Enzymatic Method) is a specialized diagnostic tool used to measure the activity of α-HBDH in human serum. This enzyme is a key indicator of tissue damage, particularly in the heart and liver. The kit employs a precise enzymatic method to ensure accurate and reliable results, making it an essential tool in clinical diagnostics.

1. The kit features a highly sensitive enzymatic reaction that allows for the precise measurement of α-HBDH levels. This sensitivity is crucial for detecting even minor elevations in enzyme activity, which can be indicative of early-stage tissue damage.
2. The composition of the kit includes two reagents, R1 and R2, which are carefully formulated to optimize the enzymatic reaction. Reagent R1 contains potassium dihydrogen phosphate and α-ketobutyrate, while Reagent R2 includes 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid sodium salt, reduced nicotinamide adenine dinucleotide, and sodium bicarbonate. These components work together to ensure the reaction proceeds efficiently.
3. The primary use of this kit is in the clinical setting, where it aids in the diagnosis and monitoring of conditions such as myocardial infarction and liver disease. By providing a quantitative measure of α-HBDH activity, the kit helps healthcare professionals make informed decisions about patient care.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a vital tool in clinical diagnostics. Its ability to accurately measure α-HBDH levels in human serum makes it indispensable for assessing tissue damage, particularly in the heart and liver. The kit's carefully formulated reagents ensure reliable results, making it a trusted choice for healthcare professionals.

The main features of the α-Hydroxybutyrate Dehydrogenase Assay Kit include its high sensitivity, precise enzymatic reaction, and reliable performance in clinical diagnostics.

1. The kit's high sensitivity allows for the detection of even minor elevations in α-HBDH activity, which is crucial for early diagnosis of tissue damage.
2. The enzymatic reaction is optimized for accuracy, ensuring that the results are reliable and reproducible.
3. The kit is designed for ease of use, with clear instructions and stable reagents that simplify the testing process.
4. It is compatible with a wide range of clinical analyzers, making it versatile and adaptable to different laboratory settings.
5. The kit's stability and long shelf life ensure that it remains effective over time, reducing the need for frequent replacements.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its sensitivity, accuracy, and ease of use. These features make it an essential tool for clinical laboratories, where reliable and precise diagnostic results are paramount.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings to diagnose and monitor conditions related to tissue damage, such as myocardial infarction and liver disease.

1. In the diagnosis of myocardial infarction, the kit helps detect elevated levels of α-HBDH, which is a marker of heart muscle damage.
2. It is also used in the assessment of liver disease, where α-HBDH levels can indicate hepatic damage.
3. The kit is valuable in monitoring the progression of these conditions, providing healthcare professionals with critical information for patient management.
4. It is often used in conjunction with other diagnostic tests to provide a comprehensive assessment of a patient's health.
5. The kit's reliability and accuracy make it a trusted tool in clinical laboratories worldwide.

The α-Hydroxybutyrate Dehydrogenase Assay Kit plays a crucial role in the clinical diagnosis and monitoring of tissue damage, particularly in the heart and liver. Its reliable performance and accuracy make it an indispensable tool for healthcare professionals.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use.

1. Product specifications should be carefully reviewed to ensure the kit meets the laboratory's requirements, including sensitivity, accuracy, and compatibility with existing equipment.
2. Different types of kits may be available, so it is important to choose one that aligns with the specific diagnostic needs of the laboratory.
3. Budget considerations are crucial, as the cost of the kit should be balanced against its performance and the volume of tests it can perform.
4. The intended use of the kit, whether for routine diagnostics or specialized testing, should guide the purchasing decision to ensure the kit is fit for purpose.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, laboratories can ensure they select a kit that meets their diagnostic needs and provides reliable results.

When using the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Always handle the reagents with care, avoiding contamination and exposure to extreme temperatures.
2. Store the kit according to the manufacturer's instructions, typically in a cool, dry place, to preserve the stability of the reagents.
3. Follow the recommended testing protocol precisely to ensure accurate and reproducible results.
4. Regularly calibrate and maintain the equipment used with the kit to prevent errors in testing.
5. Dispose of used reagents and materials according to local regulations to ensure safety and environmental compliance.

Proper handling, storage, and disposal of the α-Hydroxybutyrate Dehydrogenase Assay Kit are essential for maintaining its performance and ensuring accurate diagnostic results. By adhering to these precautions, laboratories can maximize the kit's effectiveness and reliability.