The α-Hydroxybutyrate Dehydrogenase Assay Kit is a specialized diagnostic tool used to measure the activity of the enzyme α-hydroxybutyrate dehydrogenase (HBDH) in biological samples. This enzyme is a key indicator of tissue damage, particularly in the heart and liver. The kit provides a reliable and accurate method for clinicians to assess HBDH levels, aiding in the diagnosis and monitoring of various medical conditions.

1. The kit is designed with high sensitivity and specificity, ensuring accurate detection of HBDH activity. It utilizes a colorimetric method that is easy to perform and interpret, making it suitable for routine clinical use.
2. The composition of the kit includes two reagents: Reagent 1, which contains a phosphate buffer and α-ketobutyrate, and Reagent 2, which contains NADH. These components work together to facilitate the enzymatic reaction necessary for HBDH measurement.
3. The primary use of the kit is in the diagnosis and monitoring of myocardial infarction and liver diseases. By measuring HBDH levels, clinicians can gain valuable insights into the extent of tissue damage and the effectiveness of treatment strategies.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a vital tool in clinical diagnostics. Its high sensitivity and specificity make it an essential component in the assessment of tissue damage, particularly in the heart and liver. The kit's straightforward design and reliable performance ensure that it remains a trusted choice for healthcare professionals.

The α-Hydroxybutyrate Dehydrogenase Assay Kit offers high sensitivity and specificity for measuring HBDH activity. It is designed for ease of use in clinical laboratories, providing accurate and reliable results.

1. The kit employs a colorimetric method, which is both simple and effective for detecting HBDH activity. This method ensures that results are easy to interpret, even for less experienced laboratory personnel.
2. The reagents included in the kit are carefully formulated to ensure stability and consistency. Reagent 1 contains a phosphate buffer and α-ketobutyrate, while Reagent 2 contains NADH, both of which are essential for the enzymatic reaction.
3. The kit is designed to be used with standard laboratory equipment, making it accessible to a wide range of clinical settings. It requires minimal preparation and can be easily integrated into existing laboratory workflows.
4. The kit provides a high degree of accuracy, with a low margin of error. This ensures that clinicians can rely on the results for making critical diagnostic decisions.
5. The shelf life of the kit is 12 months when stored at 2°C to 8°C, ensuring that it remains effective over an extended period. This makes it a cost-effective choice for laboratories that require consistent performance over time.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its high sensitivity, ease of use, and reliability. Its carefully formulated reagents and straightforward methodology make it an indispensable tool for clinical laboratories. The kit's long shelf life and compatibility with standard equipment further enhance its appeal, ensuring that it remains a top choice for healthcare professionals.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings to diagnose and monitor conditions such as myocardial infarction and liver diseases. It provides valuable insights into tissue damage and treatment efficacy.

1. In the diagnosis of myocardial infarction, the kit is used to measure HBDH levels in serum or plasma. Elevated levels of HBDH are indicative of heart muscle damage, aiding in the early detection and management of the condition.
2. The kit is also used in the assessment of liver diseases. By measuring HBDH activity, clinicians can evaluate the extent of liver damage and monitor the progression of the disease over time.
3. In addition to its diagnostic applications, the kit is used in research settings to study the role of HBDH in various physiological and pathological processes. This contributes to a deeper understanding of tissue damage and repair mechanisms.
4. The kit is often used in conjunction with other diagnostic tests to provide a comprehensive assessment of a patient's health. This multi-faceted approach ensures that clinicians have all the information they need to make informed decisions.
5. The kit's reliability and accuracy make it a valuable tool in emergency settings, where quick and accurate diagnosis is critical. It is often used in hospitals and clinics to assess patients with suspected heart or liver conditions.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, offering valuable insights into conditions such as myocardial infarction and liver diseases. Its reliability and accuracy make it a trusted choice for healthcare professionals, while its ease of use ensures that it can be effectively integrated into a variety of clinical settings.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to consider factors such as product specifications, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product specifications: Ensure that the kit meets your laboratory's requirements in terms of sensitivity, specificity, and compatibility with existing equipment. Check the shelf life and storage conditions to ensure that the kit will remain effective over time.
2. Product type: The kit is available in different formats, including single-use and multi-use options. Choose the format that best suits your laboratory's workflow and testing volume.
3. Budget: Consider the cost of the kit in relation to your laboratory's budget. While the kit is a cost-effective choice, it is important to ensure that it provides the necessary features and performance for your specific needs.
4. Usage scenarios: The kit is suitable for a wide range of clinical settings, including hospitals, clinics, and research laboratories. Consider the specific applications for which you will be using the kit and ensure that it meets those requirements.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, budget, and intended use. By taking these factors into account, you can ensure that you select a kit that meets your laboratory's needs and provides reliable performance over time.

When using the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Storage: The kit should be stored at 2°C to 8°C to maintain the stability of the reagents. Avoid freezing the reagents, as this can affect their performance.
2. Handling: Always handle the reagents with care, using appropriate personal protective equipment (PPE) such as gloves and lab coats. Avoid contact with skin or eyes, and wash hands thoroughly after use.
3. Preparation: Follow the manufacturer's instructions carefully when preparing the reagents. Ensure that all equipment is clean and free from contaminants to avoid interference with the assay.
4. Disposal: Dispose of used reagents and materials in accordance with local regulations. Do not pour reagents down the drain or dispose of them in regular trash.
5. Quality control: Regularly perform quality control checks to ensure that the kit is functioning correctly. Use control samples to verify the accuracy and precision of the assay.

Proper handling and storage of the α-Hydroxybutyrate Dehydrogenase Assay Kit are essential for maintaining the integrity of the reagents and ensuring accurate results. By following the recommended precautions, you can minimize the risk of errors and ensure that the kit performs as expected.