The α-Hydroxybutyrate Dehydrogenase Assay Kit is a specialized diagnostic tool used to measure the activity of the α-HBDH enzyme in human serum. This enzyme is crucial in the metabolism of hydroxybutyrate, and its activity levels can provide valuable insights into a patient's health status. The kit is designed for use in clinical laboratories and offers a reliable and accurate method for enzyme activity measurement.

1. The kit features a two-reagent system that ensures precise and reproducible results. Reagent 1 contains a phosphate buffer and α-ketobutyrate, while Reagent 2 includes a Tris buffer and NADH. This combination allows for the accurate measurement of α-HBDH activity.
2. The composition of the kit is optimized for stability and performance. The buffers maintain the necessary pH levels, and the inclusion of NADH ensures that the enzymatic reaction proceeds efficiently. This makes the kit suitable for high-throughput clinical testing.
3. The primary use of the kit is to measure α-HBDH activity in human serum, which is an important diagnostic marker for conditions such as myocardial infarction and liver disease. The kit is designed to be user-friendly, with clear instructions and a straightforward protocol.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a reliable tool for measuring enzyme activity in clinical settings. Its two-reagent system ensures accuracy, and its optimized composition makes it suitable for high-throughput testing. This kit is essential for diagnosing conditions related to α-HBDH activity.

The main features of the α-Hydroxybutyrate Dehydrogenase Assay Kit include its two-reagent system, optimized composition, and high accuracy in measuring enzyme activity. It is designed for ease of use in clinical laboratories.

1. The two-reagent system ensures precise and reproducible results, making it ideal for clinical diagnostics.
2. The optimized composition, including stable buffers and NADH, allows for efficient enzymatic reactions.
3. The kit is designed for high-throughput testing, making it suitable for busy clinical laboratories.
4. It provides accurate measurement of α-HBDH activity, which is crucial for diagnosing conditions like myocardial infarction.
5. The user-friendly design includes clear instructions and a straightforward protocol, reducing the likelihood of errors.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its precision, efficiency, and ease of use. Its two-reagent system and optimized composition make it a valuable tool for clinical laboratories, ensuring accurate measurement of enzyme activity.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings to measure α-HBDH activity in human serum. This measurement is crucial for diagnosing conditions such as myocardial infarction and liver disease.

1. The kit is used in clinical laboratories to assess α-HBDH activity, which is a key diagnostic marker for myocardial infarction.
2. It is also employed in the diagnosis of liver diseases, where α-HBDH activity levels can indicate the severity of the condition.
3. The kit is used in research settings to study the role of α-HBDH in various metabolic processes.
4. It is utilized in routine health check-ups to monitor enzyme activity and detect early signs of disease.
5. The kit is also used in emergency settings to quickly assess enzyme activity in patients with suspected myocardial infarction.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, used for assessing enzyme activity in conditions like myocardial infarction and liver disease. Its applications extend to research and routine health monitoring, making it an essential tool in modern medicine.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product specifications: Ensure the kit includes all necessary components, such as Reagent 1 and Reagent 2, and that they meet the required standards for clinical use.
2. Product types: Choose between different kit sizes or configurations based on your laboratory's testing volume and requirements.
3. Budget: Consider the cost of the kit in relation to your laboratory's budget, and compare prices from different suppliers to find the best deal.
4. Intended use: Ensure the kit is suitable for your specific diagnostic needs, whether for routine testing, research, or emergency situations.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable results.

When using the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow safety protocols and handle the reagents with care to ensure accurate results and prevent contamination.

1. Always wear appropriate personal protective equipment, such as gloves and lab coats, when handling the reagents.
2. Ensure that all equipment used with the kit is clean and properly calibrated to avoid errors in measurement.
3. Store the kit according to the manufacturer's instructions to maintain the stability and effectiveness of the reagents.
4. Follow the protocol carefully to ensure accurate results, and avoid any deviations that could affect the outcome.
5. Dispose of used reagents and materials according to local regulations to prevent environmental contamination.

Using the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful attention to safety and protocol. By following the recommended precautions, you can ensure accurate results and maintain a safe working environment.