The α-Hydroxybutyrate Dehydrogenase Assay Kit is a high-quality diagnostic tool designed for the accurate measurement of α-HBDH activity in human serum. This enzyme is a key marker in assessing tissue damage, particularly in the heart, liver, and muscles. The kit employs a rate method, ensuring precise and reliable results. With its robust composition and stringent quality controls, it is widely used in clinical laboratories to support the diagnosis and monitoring of various medical conditions.

1. The kit features a two-reagent system, R1 and R2, which work synergistically to measure α-HBDH activity. R1 contains α-ketobutyrate, phosphate, and sodium azide, while R2 contains NADH. This combination ensures high sensitivity and specificity in detecting enzyme activity.
2. The composition of the kit is meticulously designed to provide accurate and reproducible results. The reagents are formulated to maintain stability and performance, even under varying laboratory conditions. The kit's linear range of 0 to 400 U/L and a correlation coefficient of r≥0.99 ensure reliable measurements across a wide range of enzyme activities.
3. The primary use of the kit is in clinical diagnostics, where it aids in the detection and monitoring of diseases related to tissue damage. Elevated levels of α-HBDH can indicate myocardial infarction, liver disease, or muscle disorders, making this kit an essential tool for healthcare professionals.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Kit, is a reliable and precise diagnostic tool used in clinical laboratories. Its two-reagent system and robust composition make it ideal for measuring α-HBDH activity in human serum. This kit is indispensable for diagnosing and monitoring conditions related to heart, liver, and muscle diseases, providing healthcare professionals with accurate and actionable insights.

The α-Hydroxybutyrate Dehydrogenase Assay Kit offers high accuracy, precision, and a wide linear range, making it a reliable tool for measuring α-HBDH activity in clinical settings.

1. The kit employs a rate method, ensuring precise measurement of α-HBDH activity. This method is highly sensitive and specific, providing accurate results even at low enzyme concentrations.
2. The two-reagent system (R1 and R2) is designed for ease of use and stability. R1 contains α-ketobutyrate, phosphate, and sodium azide, while R2 contains NADH, ensuring optimal performance and reliability.
3. The kit's linear range of 0 to 400 U/L and a correlation coefficient of r≥0.99 ensure that it can accurately measure a wide range of enzyme activities, making it suitable for various clinical applications.
4. The kit is designed for high precision, with a batch-to-batch variation of ≤8% and a within-batch coefficient of variation (CV) of ≤6%. This ensures consistent and reproducible results across different laboratory settings.
5. The kit is easy to use, with clear instructions and minimal preparation required. It is compatible with most automated analyzers, making it a convenient choice for busy clinical laboratories.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its precision, accuracy, and ease of use. Its two-reagent system and wide linear range make it a versatile tool for clinical diagnostics, providing reliable results for healthcare professionals.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is widely used in clinical laboratories for diagnosing and monitoring diseases related to tissue damage, such as myocardial infarction, liver disease, and muscle disorders.

1. The kit is commonly used in the diagnosis of myocardial infarction. Elevated levels of α-HBDH in serum can indicate heart muscle damage, making this kit an essential tool for cardiologists.
2. In liver disease, the kit helps monitor enzyme levels, which can indicate liver damage or dysfunction. This is particularly useful in cases of hepatitis or cirrhosis.
3. The kit is also used in the diagnosis of muscle disorders, such as muscular dystrophy or rhabdomyolysis. Elevated α-HBDH levels can indicate muscle tissue damage, aiding in the diagnosis and management of these conditions.
4. The kit is used in routine health check-ups to assess overall tissue health. It can help detect early signs of tissue damage, allowing for timely intervention and treatment.
5. In research settings, the kit is used to study the role of α-HBDH in various metabolic processes. It provides valuable insights into the biochemical pathways involved in tissue damage and repair.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, used for diagnosing and monitoring a range of diseases related to tissue damage. Its applications extend from cardiology to hepatology and beyond, making it an indispensable resource for healthcare professionals.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, consider factors such as product specifications, budget, and intended use to ensure it meets your laboratory's needs.

1. Product specifications: Ensure the kit meets your laboratory's requirements for accuracy, precision, and linear range. Check the reagent composition and stability to ensure compatibility with your equipment.
2. Product type: Choose between manual and automated kits based on your laboratory's workflow. Automated kits are more convenient for high-throughput laboratories, while manual kits may be suitable for smaller labs.
3. Budget: Consider the cost per test and the overall budget for your laboratory. Compare prices from different suppliers to find the best value without compromising on quality.
4. Usage scenarios: Assess the kit's suitability for your specific clinical applications. Ensure it can handle the volume and type of samples you typically process.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product specifications, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable results for your clinical applications.

Proper handling and storage of the α-Hydroxybutyrate Dehydrogenase Assay Kit are essential to ensure accurate results and maintain reagent stability.

1. Storage: Store the kit at the recommended temperature (usually 2-8°C) to maintain reagent stability. Avoid freezing the reagents, as this can affect their performance.
2. Handling: Use aseptic techniques when handling the reagents to prevent contamination. Ensure all equipment is clean and properly calibrated before use.
3. Expiration: Check the expiration date on the kit before use. Do not use expired reagents, as this can lead to inaccurate results.
4. Disposal: Dispose of used reagents and samples according to local regulations. Follow proper biohazard disposal protocols to ensure safety.
5. Training: Ensure all laboratory personnel are trained in the proper use of the kit. Follow the manufacturer's instructions carefully to avoid errors.

The α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful handling and storage to ensure accurate results. Proper storage, handling, and disposal practices, along with adequate training, are essential to maintain the kit's performance and ensure the safety of laboratory personnel.