The α-Hydroxybutyrate Dehydrogenase Assay Kit (DGKC Method) is a specialized diagnostic tool used to measure the activity of α-hydroxybutyrate dehydrogenase in biological samples. This enzyme is crucial in metabolic processes, and its levels can provide valuable insights into the health status of an individual, particularly in relation to cardiac and hepatic function. The kit is designed for use in clinical laboratories and offers a reliable and precise method for enzyme quantification.

1. The kit features a high level of sensitivity and specificity, ensuring accurate detection of α-HBDH levels even in low-concentration samples. Its robust design minimizes interference from other substances, making it a reliable choice for clinical diagnostics.
2. The composition of the kit includes two key reagents: R1 and R2. R1 contains phosphate buffer and α-ketobutyrate, while R2 consists of phosphate buffer and NADH. These components are carefully formulated to ensure optimal enzyme activity measurement.
3. The primary use of this kit is to quantitatively determine α-HBDH levels in various biological specimens. This information is crucial for diagnosing and monitoring conditions related to heart and liver health, as α-HBDH is a marker for tissue damage in these organs.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is a vital tool in clinical diagnostics. Its precise formulation and reliable performance make it an essential component in the assessment of enzyme activity, particularly in the context of cardiac and hepatic health. The kit's design ensures accurate and consistent results, providing healthcare professionals with the information they need to make informed decisions about patient care.

The α-Hydroxybutyrate Dehydrogenase Assay Kit offers a range of features designed to ensure accurate and reliable measurement of enzyme activity. Its high sensitivity, specificity, and ease of use make it a preferred choice for clinical laboratories.

1. High Sensitivity: The kit is capable of detecting even low levels of α-HBDH, ensuring accurate results across a wide range of sample concentrations.
2. Specificity: The formulation of the reagents minimizes interference from other substances, providing precise measurements of α-HBDH activity.
3. Ease of Use: The kit is designed for straightforward application in clinical settings, with clear instructions and minimal preparation required.
4. Reliability: Consistent performance and reproducibility are key features of this kit, making it a trusted tool for enzyme activity measurement.
5. Versatility: The kit can be used with a variety of biological specimens, making it suitable for a wide range of diagnostic applications.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its high sensitivity, specificity, and ease of use. These features, combined with its reliability and versatility, make it an indispensable tool in clinical diagnostics. Whether used in routine testing or specialized research, this kit delivers consistent and accurate results, supporting healthcare professionals in their diagnostic efforts.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is widely used in clinical settings to assess enzyme activity, particularly in the diagnosis and monitoring of heart and liver conditions. Its precise measurement capabilities make it a valuable tool in patient care.

1. Cardiac Health: The kit is used to measure α-HBDH levels, which can indicate myocardial damage. This information is crucial for diagnosing conditions such as myocardial infarction.
2. Liver Function: Elevated levels of α-HBDH can also signal liver damage, making the kit useful in the assessment of hepatic health and the diagnosis of liver diseases.
3. Monitoring Treatment: The kit can be used to monitor the effectiveness of treatments for heart and liver conditions, providing valuable feedback on patient progress.
4. Research Applications: Beyond clinical diagnostics, the kit is also used in research settings to study enzyme activity and its implications in various metabolic processes.
5. Routine Screening: The kit is suitable for routine screening in clinical laboratories, helping to identify potential health issues before they become more serious.

The α-Hydroxybutyrate Dehydrogenase Assay Kit plays a crucial role in clinical diagnostics, particularly in the assessment of cardiac and hepatic health. Its ability to provide precise and reliable measurements of enzyme activity makes it an essential tool for diagnosing and monitoring a range of medical conditions. Whether used in routine screening or specialized research, this kit offers valuable insights into patient health.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product Specifications: Ensure that the kit meets the required specifications for sensitivity, specificity, and accuracy. Check the composition of the reagents and their compatibility with your laboratory equipment.
2. Product Types: There may be different versions of the kit available, each designed for specific applications. Choose the type that best suits your diagnostic needs.
3. Budget Considerations: Evaluate the cost of the kit in relation to your budget. Consider the long-term value and reliability of the product when making your decision.
4. Intended Use: Determine the primary use of the kit, whether for routine screening, specialized diagnostics, or research. This will help you select the most appropriate product for your needs.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you select the most suitable kit for your clinical or research needs, ultimately supporting accurate and reliable diagnostic outcomes.

When using the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow specific precautions to ensure accurate results and maintain safety. These precautions include proper handling, storage, and disposal of reagents.

1. Proper Handling: Always wear appropriate personal protective equipment (PPE) when handling the reagents. Avoid direct contact with skin and eyes, and ensure proper ventilation in the laboratory.
2. Storage Conditions: Store the kit according to the manufacturer's instructions, typically at 2-8°C. Ensure that the reagents are kept in their original containers and protected from light.
3. Disposal: Dispose of used reagents and materials in accordance with local regulations and laboratory safety protocols. Do not pour reagents down the drain.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure accurate measurements. Follow the manufacturer's guidelines for calibration procedures.
5. Contamination Prevention: Take steps to prevent contamination of the reagents, such as using clean pipettes and avoiding cross-contamination between samples.

Using the α-Hydroxybutyrate Dehydrogenase Assay Kit requires adherence to specific precautions to ensure both accurate results and laboratory safety. Proper handling, storage, and disposal of reagents are essential, as is regular equipment calibration and contamination prevention. By following these guidelines, you can maintain the integrity of your diagnostic results and ensure a safe working environment.