The α-Hydroxybutyrate Dehydrogenase Assay Kit is a sophisticated diagnostic tool used in clinical settings to measure the activity of the enzyme α-hydroxybutyrate dehydrogenase in human serum. This enzyme is a marker for cardiac and liver health, and its levels can provide critical insights into tissue damage or disease progression. The kit employs a continuous monitoring method, ensuring high accuracy and reliability in results.

1. The kit features a highly sensitive and specific assay method, ensuring precise measurement of HBDH activity. The continuous monitoring approach allows for real-time data collection, enhancing the reliability of the results.
2. The composition of the kit includes two reagents: R1, which contains a phosphate buffer and 2-oxobutyrate, and R2, which includes NADH. These components work synergistically to facilitate the enzymatic reaction necessary for HBDH activity measurement.
3. The primary use of this kit is in clinical laboratories for the quantitative determination of HBDH activity in human serum. This measurement is crucial for diagnosing and monitoring conditions related to cardiac and liver function.

The α-Hydroxybutyrate Dehydrogenase Assay Kit, also known as the HBDH Assay Kit, is an essential tool in clinical diagnostics. Its ability to accurately measure HBDH activity in serum makes it invaluable for assessing cardiac and liver health. The kit's design, featuring a continuous monitoring method and carefully formulated reagents, ensures reliable and precise results, making it a trusted choice for healthcare professionals.

The main features of the α-Hydroxybutyrate Dehydrogenase Assay Kit include its high sensitivity, specificity, and the use of a continuous monitoring method for accurate HBDH activity measurement.

1. High Sensitivity: The kit is designed to detect even low levels of HBDH activity, making it suitable for early detection of tissue damage or disease.
2. Specificity: The assay is highly specific to HBDH, reducing the likelihood of cross-reactivity with other enzymes and ensuring accurate results.
3. Continuous Monitoring: The continuous monitoring method allows for real-time data collection, providing a dynamic view of enzyme activity.
4. Reagent Stability: The reagents are formulated for long-term stability, ensuring consistent performance over time.
5. Ease of Use: The kit is designed for ease of use in clinical laboratories, with clear instructions and minimal preparation required.

The α-Hydroxybutyrate Dehydrogenase Assay Kit stands out for its high sensitivity, specificity, and the use of a continuous monitoring method. These features make it an indispensable tool for clinical laboratories, providing accurate and reliable measurements of HBDH activity. The kit's design ensures ease of use and long-term stability, making it a preferred choice for healthcare professionals.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is primarily used in clinical settings for the diagnosis and monitoring of cardiac and liver diseases. It provides critical insights into tissue damage and disease progression.

1. Cardiac Disease Diagnosis: The kit is used to measure HBDH levels in serum, which can indicate myocardial infarction or other cardiac conditions.
2. Liver Function Assessment: Elevated HBDH levels can also indicate liver damage or disease, making the kit useful for liver function tests.
3. Monitoring Disease Progression: The kit allows for the continuous monitoring of HBDH activity, providing valuable data on the progression of cardiac or liver diseases.
4. Research Applications: Beyond clinical diagnostics, the kit is also used in research settings to study the role of HBDH in various physiological and pathological processes.
5. Quality Control: The kit is employed in quality control processes in clinical laboratories to ensure the accuracy and reliability of HBDH activity measurements.

The α-Hydroxybutyrate Dehydrogenase Assay Kit is a versatile tool in clinical diagnostics, primarily used for the diagnosis and monitoring of cardiac and liver diseases. Its ability to provide accurate and reliable measurements of HBDH activity makes it invaluable for healthcare professionals. Additionally, the kit finds applications in research and quality control, further underscoring its importance in the medical field.

When purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to consider product parameters, types, budget, and intended use to ensure the best fit for your clinical laboratory needs.

1. Product Parameters: Evaluate the sensitivity, specificity, and stability of the kit to ensure it meets your laboratory's requirements.
2. Product Types: Consider the different types of kits available, such as those with varying reagent concentrations or additional features, to find the most suitable option.
3. Budget: Determine your budget and compare prices from different suppliers to find a kit that offers the best value for money.
4. Intended Use: Assess the specific applications for which you will use the kit, such as cardiac or liver disease diagnosis, to ensure it aligns with your clinical needs.

Purchasing the α-Hydroxybutyrate Dehydrogenase Assay Kit requires careful consideration of product parameters, types, budget, and intended use. By evaluating these factors, you can select a kit that best meets the needs of your clinical laboratory, ensuring accurate and reliable HBDH activity measurements.

When using the α-Hydroxybutyrate Dehydrogenase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain reagent stability.

1. Storage Conditions: Store the kit at the recommended temperature to maintain reagent stability and ensure accurate results.
2. Handling: Follow proper handling procedures to avoid contamination or degradation of the reagents.
3. Calibration: Regularly calibrate the equipment used with the kit to ensure accurate measurements.
4. Safety: Wear appropriate personal protective equipment (PPE) when handling the reagents to avoid exposure.
5. Disposal: Dispose of used reagents and materials according to local regulations to minimize environmental impact.

Proper handling and storage of the α-Hydroxybutyrate Dehydrogenase Assay Kit are crucial for maintaining reagent stability and ensuring accurate results. By following the recommended precautions, including proper storage, handling, calibration, safety measures, and disposal procedures, you can maximize the performance and longevity of the kit in your clinical laboratory.