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α-Amylase Assay Kit (4-Nitrophenyl-1,4-α-D-Maltoheptaoside Substrate Method)
The α-Amylase Assay Kit consists of two main reagents: R1 and R2. R1 contains a buffer solution with a concentration of 100-500 mmol/L, α-glucosidase at 2-5 KU/L, sodium chloride at 50-100 mmol/L, and a preservative at 0.10-3.0%. R2 includes a buffer solution at 100-500 mmol/L and 4-Nitrophenyl-1,4-α-D-Maltoheptaoside (EPS-G7) at 6-45 mmol/L. These components work together to facilitate the accurate measurement of α-amylase activity in serum samples.
Product English Name:
α-Amylase Assay Kit (4-Nitrophenyl-1,4-α-D-Maltoheptaoside Substrate Method)
Alias:
Amylase Test Kit,EPS-G7 Amylase Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of α-amylase activity in human serum samples. It is primarily used in clinical settings to aid in the diagnosis of pancreatic diseases, providing critical information for patient management and treatment decisions.
Product Introduction

The α-Amylase Assay Kit is a sophisticated diagnostic tool used to measure the activity of α-amylase in serum samples. This enzyme plays a crucial role in carbohydrate metabolism, and its levels can be indicative of various pancreatic conditions. The kit employs a colorimetric method using 4-Nitrophenyl-1,4-α-D-Maltoheptaoside (EPS-G7) as a substrate, ensuring high sensitivity and specificity in detecting α-amylase activity.

1. The kit features a highly sensitive and specific assay method, utilizing EPS-G7 as a substrate to ensure accurate detection of α-amylase activity. This method is known for its reliability and precision in clinical diagnostics.
2. The composition of the kit includes two reagents, R1 and R2, each containing specific components that work synergistically to facilitate the enzymatic reaction. R1 contains α-glucosidase and a buffer solution, while R2 includes the EPS-G7 substrate.
3. The primary use of this kit is in the clinical diagnosis of pancreatic diseases. By measuring α-amylase activity in serum samples, healthcare providers can gain valuable insights into a patient's pancreatic health and make informed treatment decisions.

The α-Amylase Assay Kit, also known as the Amylase Test Kit or EPS-G7 Amylase Kit, is a vital tool in clinical diagnostics. Its ability to accurately measure α-amylase activity in serum samples makes it indispensable for the diagnosis of pancreatic diseases. The kit's robust design and reliable performance ensure that healthcare providers can trust the results it provides, ultimately leading to better patient outcomes.

Main Features

The α-Amylase Assay Kit offers a range of features designed to ensure accurate and reliable measurement of α-amylase activity in serum samples. These features include high sensitivity, specificity, and ease of use, making it a valuable tool in clinical diagnostics.

1. High Sensitivity: The kit is designed to detect even low levels of α-amylase activity, ensuring that subtle changes in enzyme levels are not missed.
2. Specificity: The use of EPS-G7 as a substrate ensures that the assay is specific to α-amylase, reducing the risk of cross-reactivity with other enzymes.
3. Ease of Use: The kit is user-friendly, with clear instructions and a straightforward protocol that can be easily followed by laboratory personnel.
4. Reliability: The kit has been rigorously tested to ensure consistent performance, providing reliable results that clinicians can trust.
5. Versatility: The kit can be used in a variety of clinical settings, making it a versatile tool for diagnosing pancreatic diseases.

The α-Amylase Assay Kit stands out for its high sensitivity, specificity, and ease of use. These features make it an invaluable tool in clinical diagnostics, particularly for the diagnosis of pancreatic diseases. The kit's reliability and versatility ensure that it can be used effectively in a wide range of clinical settings, providing accurate and trustworthy results.

Clinical Applications

The α-Amylase Assay Kit is widely used in clinical settings for the diagnosis of pancreatic diseases. Its ability to accurately measure α-amylase activity in serum samples makes it a critical tool for healthcare providers.

1. Pancreatic Disease Diagnosis: The kit is primarily used to diagnose pancreatic diseases, such as pancreatitis, by measuring α-amylase activity in serum samples.
2. Monitoring Treatment: The kit can be used to monitor the effectiveness of treatment for pancreatic diseases, providing valuable feedback on patient progress.
3. Research Applications: The kit is also used in research settings to study the role of α-amylase in various physiological and pathological processes.
4. Clinical Trials: The kit is often used in clinical trials to assess the efficacy of new treatments for pancreatic diseases.
5. Routine Screening: The kit can be used for routine screening of patients at risk for pancreatic diseases, allowing for early detection and intervention.

The α-Amylase Assay Kit is a versatile tool with a wide range of clinical applications. Its ability to accurately measure α-amylase activity makes it indispensable for the diagnosis and monitoring of pancreatic diseases. Whether used in routine screening, research, or clinical trials, the kit provides reliable and actionable results that can improve patient outcomes.

Purchasing Guide

When purchasing the α-Amylase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use to ensure that the kit meets your specific needs.

1. Product Specifications: Ensure that the kit's specifications, such as sensitivity, specificity, and shelf life, meet your laboratory's requirements.
2. Product Types: Consider the different types of kits available, such as those designed for high-throughput screening or those with extended shelf life, to find the best fit for your needs.
3. Budget: Determine your budget and compare prices from different suppliers to find a kit that offers the best value for your money.
4. Intended Use: Consider the specific applications for which you will be using the kit, such as routine screening, research, or clinical trials, to ensure that the kit is suitable for your intended use.

Purchasing the α-Amylase Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking the time to evaluate these factors, you can ensure that you select a kit that meets your specific needs and provides reliable results for your clinical or research applications.

 Precautions

When using the α-Amylase Assay Kit, it is important to follow all safety precautions and handling instructions to ensure accurate results and prevent contamination or injury.

1. Proper Storage: Store the kit at the recommended temperature to maintain the stability and effectiveness of the reagents.
2. Handling: Handle all reagents with care, using appropriate personal protective equipment (PPE) to avoid exposure to harmful substances.
3. Contamination Prevention: Use sterile techniques and avoid cross-contamination between samples to ensure accurate results.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure consistent and accurate measurements.
5. Disposal: Dispose of all used reagents and materials according to local regulations and guidelines to prevent environmental contamination.

Using the α-Amylase Assay Kit safely and effectively requires adherence to proper storage, handling, and disposal procedures. By following these precautions, you can ensure the accuracy of your results and protect yourself and the environment from potential harm. Always prioritize safety and compliance with regulations when working with diagnostic kits.

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