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α-Amylase Assay Kit (E-pNP-G7 Method)
The α-Amylase Assay Kit is composed of two main reagents: Reagent R1 and Reagent R2. Reagent R1 contains glucose glycosidase (>4500KU/L) and magnesium sulfate (10mmol/L), while Reagent R2 includes a buffer solution (100mmol/L), sodium chloride (50mmol/L), and ethylene-pNP-G7 (50mmol/L). The detection system is traceable to the Internal Master. It is important to note that components from different batch numbers are not interchangeable.
Product English Name:
α-Amylase Assay Kit (E-pNP-G7 Method)
Alias:
Amylase Test Kit,E-pNP-G7 Amylase Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of α-amylase (α-AMY) activity in human samples, including serum, plasma, or urine. It is widely used in clinical diagnostics to assess pancreatic function and diagnose related disorders.
Product Introduction

The α-Amylase Assay Kit (E-pNP-G7 Method) is a highly reliable and accurate diagnostic tool used to measure α-amylase activity in human samples. This enzyme plays a crucial role in carbohydrate metabolism, and its levels are often indicative of pancreatic health. The kit employs a colorimetric method, ensuring precise and reproducible results. It is an essential tool in clinical laboratories for diagnosing and monitoring conditions such as pancreatitis, salivary gland disorders, and other related diseases.

1. The kit features a robust detection system that ensures high sensitivity and specificity. The use of ethylene-pNP-G7 as a substrate allows for accurate measurement of α-amylase activity, making it suitable for both routine and research applications.
2. The composition of the kit includes two reagents: Reagent R1, which contains glucose glycosidase and magnesium sulfate, and Reagent R2, which consists of a buffer solution, sodium chloride, and ethylene-pNP-G7. These components work together to provide a stable and reliable assay environment.
3. The primary use of this kit is to quantitatively determine α-amylase activity in human serum, plasma, or urine. It is particularly useful in diagnosing pancreatic disorders, monitoring disease progression, and evaluating the effectiveness of treatment.

The α-Amylase Assay Kit (E-pNP-G7 Method) is a vital tool in clinical diagnostics, offering a reliable and accurate means of measuring α-amylase activity. Its robust design and precise detection system make it indispensable for diagnosing and monitoring pancreatic and salivary gland disorders. The kit's composition and ease of use further enhance its appeal to both clinical and research laboratories.

Main Features

The α-Amylase Assay Kit offers high sensitivity, specificity, and reproducibility in measuring α-amylase activity. Its colorimetric method ensures accurate results, making it suitable for both clinical and research applications.

1. High Sensitivity: The kit is designed to detect even low levels of α-amylase activity, ensuring accurate diagnosis and monitoring of pancreatic disorders.
2. Specificity: The use of ethylene-pNP-G7 as a substrate ensures that the assay is specific to α-amylase, minimizing interference from other enzymes.
3. Reproducibility: The kit provides consistent results across different batches and laboratories, thanks to its traceable detection system.
4. Ease of Use: The kit is user-friendly, with clear instructions and a straightforward protocol, making it accessible to both experienced and novice users.
5. Versatility: The kit can be used with various human samples, including serum, plasma, and urine, making it a versatile tool in clinical diagnostics.

The α-Amylase Assay Kit stands out for its high sensitivity, specificity, and reproducibility. Its user-friendly design and versatility make it an essential tool for clinical laboratories, ensuring accurate and reliable results in the diagnosis and monitoring of pancreatic disorders.

Clinical Applications

The α-Amylase Assay Kit is widely used in clinical settings to diagnose and monitor pancreatic disorders, such as pancreatitis, and salivary gland diseases. It is also used in research to study carbohydrate metabolism and enzyme activity.

1. Diagnosis of Pancreatitis: The kit is commonly used to measure α-amylase levels in patients suspected of having acute or chronic pancreatitis, aiding in accurate diagnosis.
2. Monitoring Disease Progression: Regular measurement of α-amylase activity helps in monitoring the progression of pancreatic disorders and evaluating the effectiveness of treatment.
3. Salivary Gland Disorders: The kit is also used to diagnose disorders of the salivary glands, where abnormal α-amylase levels may be indicative of disease.
4. Research Applications: In research settings, the kit is used to study the role of α-amylase in carbohydrate metabolism and its interaction with other enzymes.
5. Clinical Trials: The kit is employed in clinical trials to assess the efficacy of new treatments for pancreatic and salivary gland disorders.

The α-Amylase Assay Kit is a versatile tool in both clinical and research settings. Its ability to accurately measure α-amylase activity makes it indispensable for diagnosing and monitoring pancreatic and salivary gland disorders, as well as for advancing research in carbohydrate metabolism.

Purchasing Guide

When purchasing the α-Amylase Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product Specifications: Ensure the kit meets the required sensitivity, specificity, and reproducibility standards for your laboratory's diagnostic needs.
2. Product Types: Choose between different kit formats (e.g., single-use vs. bulk) based on your laboratory's volume and frequency of use.
3. Budget: Consider the cost per test and overall budget when selecting a kit, ensuring it provides good value without compromising quality.
4. Intended Use: Determine the primary application (clinical diagnostics, research, or both) to select a kit that best suits your laboratory's requirements.

Selecting the right α-Amylase Assay Kit involves careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable and accurate results.

 Precautions

Proper handling and storage of the α-Amylase Assay Kit are essential to ensure accurate results and prolong the kit's shelf life. Follow all instructions carefully to avoid contamination and ensure reliable performance.

1. Storage Conditions: Store the kit at the recommended temperature to maintain the stability of the reagents. Avoid freezing or exposing the kit to extreme temperatures.
2. Handling: Use sterile techniques when handling the reagents to prevent contamination. Always wear appropriate personal protective equipment (PPE).
3. Expiration Date: Do not use the kit beyond its expiration date, as this may affect the accuracy and reliability of the results.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure accurate measurements.
5. Disposal: Dispose of used reagents and samples according to local regulations and laboratory safety protocols.

To ensure the α-Amylase Assay Kit performs reliably, it is crucial to follow proper storage, handling, and disposal procedures. Adhering to these precautions will help maintain the kit's integrity and ensure accurate and consistent results in your laboratory.

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