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α-Amylase Assay Kit (Gal-G5-pNP Substrate Method)
The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) consists of two main reagents: Reagent 1 (R1) containing glucoamylase (28U/mL) and α-glucosidase (14U/mL), and Reagent 2 (R2) containing capped-P-nitrophenyl-α-maltopentaose (Gal-G5-pNP) at a concentration of 3.34mg/mL. This kit is designed for the quantitative determination of α-amylase activity in serum or plasma samples.
Product English Name:
α-Amylase Assay Kit (Gal-G5-pNP Substrate Method)
Alias:
Amylase Test Kit,Gal-G5-pNP Kit
Intended Use:
This kit is primarily used for the quantitative determination of α-amylase activity in serum or plasma. It is widely utilized in clinical diagnostics to assess pancreatic function and to diagnose conditions such as pancreatitis, salivary gland disorders, and other related diseases.
Product Introduction

The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) is a highly sensitive and specific tool for measuring α-amylase activity in biological samples. It employs a unique substrate, Gal-G5-pNP, which allows for precise and accurate quantification of enzyme activity. This kit is essential for clinical laboratories and research institutions focusing on pancreatic and salivary gland disorders.

1. The kit features a unique substrate, Gal-G5-pNP, which provides high specificity and sensitivity in detecting α-amylase activity. This substrate is designed to react specifically with α-amylase, ensuring accurate results.
2. The kit consists of two reagents: Reagent 1 (R1) contains glucoamylase and α-glucosidase, which work together to break down the substrate, while Reagent 2 (R2) contains the Gal-G5-pNP substrate. The combination of these reagents ensures a reliable and reproducible assay.
3. The primary use of this kit is in clinical diagnostics, where it is employed to measure α-amylase activity in serum or plasma. This measurement is crucial for diagnosing pancreatic and salivary gland disorders, as well as monitoring the progression of these conditions.

The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) is a specialized tool designed for the precise measurement of α-amylase activity. Its unique substrate and reagent composition make it an indispensable resource for clinical laboratories. Whether you're diagnosing pancreatic disorders or conducting research, this kit offers the accuracy and reliability you need.

Main Features

The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) offers high sensitivity and specificity in measuring α-amylase activity. Its unique substrate and reagent composition ensure accurate and reproducible results.

1. High Sensitivity: The kit's unique substrate, Gal-G5-pNP, allows for the detection of even low levels of α-amylase activity, making it ideal for clinical diagnostics.
2. Specificity: The substrate is designed to react specifically with α-amylase, minimizing interference from other enzymes and ensuring accurate results.
3. Reproducibility: The combination of Reagent 1 and Reagent 2 ensures consistent and reliable assay performance, even across different batches.
4. Ease of Use: The kit is designed for straightforward use in clinical laboratories, with clear instructions and minimal preparation required.
5. Wide Application: This kit is suitable for a variety of clinical and research applications, including the diagnosis of pancreatic and salivary gland disorders.

The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) stands out for its high sensitivity, specificity, and ease of use. Whether you're a clinician or a researcher, this kit provides the tools you need to accurately measure α-amylase activity and make informed decisions.

Clinical Applications

The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) is widely used in clinical settings to diagnose and monitor pancreatic and salivary gland disorders. Its high sensitivity and specificity make it a valuable tool in clinical diagnostics.

1. Pancreatitis Diagnosis: The kit is commonly used to diagnose acute and chronic pancreatitis by measuring α-amylase activity in serum or plasma.
2. Salivary Gland Disorders: It is also employed to diagnose disorders of the salivary glands, such as sialadenitis, by assessing α-amylase levels.
3. Monitoring Disease Progression: The kit is used to monitor the progression of pancreatic and salivary gland disorders, helping clinicians adjust treatment plans as needed.
4. Research Applications: In addition to clinical diagnostics, the kit is used in research settings to study the role of α-amylase in various physiological and pathological processes.
5. Quality Control: The kit is also used in quality control processes in clinical laboratories to ensure the accuracy and reliability of α-amylase measurements.

The α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) is a versatile tool in clinical diagnostics, offering precise measurements of α-amylase activity. Its applications range from diagnosing pancreatitis to monitoring disease progression, making it an essential resource for clinicians and researchers alike.

Purchasing Guide

When purchasing the α-Amylase Assay Kit (Gal-G5-pNP Substrate Method), consider factors such as product specifications, types, budget, and intended use to ensure you select the right kit for your needs.

1. Product Specifications: Review the kit's specifications, including sensitivity, specificity, and reagent composition, to ensure it meets your requirements.
2. Product Types: Consider the different types of kits available, such as those designed for high-throughput screening or research purposes, and choose the one that best fits your application.
3. Budget: Determine your budget and compare prices from different suppliers to find a kit that offers the best value for your money.
4. Intended Use: Consider the specific clinical or research applications for which you will use the kit, and ensure that the kit you choose is suitable for those purposes.

Selecting the right α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) involves careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you choose a kit that meets your needs and provides reliable results.

 Precautions

When using the α-Amylase Assay Kit (Gal-G5-pNP Substrate Method), it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to maintain the stability and activity of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Handle the reagents with care, using appropriate protective equipment such as gloves and lab coats. Avoid contamination by using sterile techniques.
3. Calibration: Regularly calibrate your equipment, such as spectrophotometers or microplate readers, to ensure accurate measurements.
4. Sample Preparation: Follow the recommended procedures for sample preparation to avoid errors in α-amylase activity measurement.
5. Disposal: Dispose of used reagents and samples according to local regulations and guidelines to ensure safety and environmental compliance.

Proper handling and storage of the α-Amylase Assay Kit (Gal-G5-pNP Substrate Method) are crucial for obtaining accurate results. By following the recommended precautions, you can ensure the reliability of your measurements and maintain the integrity of the reagents.

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