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α-Amylase Assay Kit (CNPG2 Substrate Method)
The α-Amylase Assay Kit is composed of a carefully balanced mixture of reagents, including MES buffer (pH 6.1), sodium chloride, calcium chloride, sodium hydroxide, 2-chloro-4-nitrophenyl-α-galactosyl-maltoside, Triton X-100, and Proclin 300. The kit also includes calibrators and control samples with specific concentrations of α-amylase and bovine serum albumin, ensuring accurate and reliable measurements of α-amylase activity in human serum.
Product English Name:
α-Amylase Assay Kit (CNPG2 Substrate Method)
Alias:
Amylase Test Kit,CNPG2 Amylase Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of α-amylase activity in human serum. It is widely used in clinical laboratories to assess pancreatic function and diagnose conditions related to abnormal amylase levels, such as pancreatitis and other pancreatic disorders.
Product Introduction

The α-Amylase Assay Kit (CNPG2 Substrate Method) is a high-quality diagnostic tool used to measure the activity of α-amylase in human serum. This enzyme plays a crucial role in carbohydrate metabolism, and its levels can provide valuable insights into pancreatic health. The kit is designed for ease of use, offering precise and reproducible results, making it an essential tool in clinical diagnostics.

1. The kit features a unique CNPG2 substrate, which ensures high sensitivity and specificity in detecting α-amylase activity. This substrate reacts with α-amylase to produce a measurable color change, allowing for accurate quantification.
2. The composition of the kit includes a balanced mixture of reagents, such as MES buffer, sodium chloride, and calcium chloride, which create an optimal environment for the enzymatic reaction. Additionally, the inclusion of Proclin 300 ensures the stability and longevity of the reagents.
3. The primary use of this kit is to quantitatively measure α-amylase activity in human serum, aiding in the diagnosis of pancreatic disorders. It is particularly useful in clinical settings where rapid and accurate assessment of pancreatic function is required.

The α-Amylase Assay Kit, also known as the CNPG2 Amylase Kit, is a reliable and efficient tool for measuring α-amylase activity in human serum. Its unique composition and high sensitivity make it an indispensable resource in clinical diagnostics, particularly for assessing pancreatic health.

Main Features

The α-Amylase Assay Kit offers high sensitivity, specificity, and ease of use, making it a preferred choice for clinical laboratories. Its robust design ensures accurate and reproducible results, essential for diagnosing pancreatic disorders.

1. High Sensitivity: The CNPG2 substrate ensures that even low levels of α-amylase activity can be detected, providing accurate results in clinical samples.
2. Specificity: The kit is designed to specifically react with α-amylase, minimizing interference from other enzymes or substances in the serum.
3. Stability: The inclusion of Proclin 300 and other stabilizing agents ensures that the reagents remain effective over a long period, reducing the need for frequent replacements.
4. Ease of Use: The kit is designed for straightforward application, with clear instructions and minimal preparation required, making it accessible for both experienced and novice users.
5. Reproducibility: The consistent formulation of the reagents and calibrators ensures that results are reproducible across different batches and laboratories.

The α-Amylase Assay Kit stands out for its high sensitivity, specificity, and ease of use. Its robust design and reliable performance make it an essential tool for clinical laboratories, ensuring accurate and reproducible results in the diagnosis of pancreatic disorders.

Clinical Applications

The α-Amylase Assay Kit is widely used in clinical settings to diagnose and monitor pancreatic disorders, such as pancreatitis, by measuring α-amylase activity in human serum.

1. Diagnosis of Pancreatitis: Elevated levels of α-amylase in serum are a key indicator of acute pancreatitis, making this kit essential for early diagnosis and treatment.
2. Monitoring Pancreatic Function: The kit is used to monitor α-amylase levels in patients with chronic pancreatitis or other pancreatic disorders, helping to assess the effectiveness of treatment.
3. Post-Surgical Monitoring: After pancreatic surgery, the kit can be used to monitor α-amylase levels, ensuring that the pancreas is functioning correctly and detecting any complications early.
4. Research Applications: The kit is also used in research settings to study the role of α-amylase in various metabolic processes and its implications in different diseases.
5. Routine Health Checkups: In some cases, the kit is used in routine health checkups to screen for potential pancreatic issues, especially in patients with a family history of pancreatic disorders.

The α-Amylase Assay Kit is a versatile tool in clinical diagnostics, primarily used for diagnosing and monitoring pancreatic disorders. Its applications extend from routine health checkups to post-surgical monitoring, making it an invaluable resource in both clinical and research settings.

Purchasing Guide

When purchasing the α-Amylase Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product Specifications: Ensure that the kit's sensitivity, specificity, and stability meet the requirements of your laboratory. Check the concentration of reagents and the shelf life of the product.
2. Product Types: There may be different versions of the kit available, such as those with different substrate concentrations or additional features. Choose the one that best fits your diagnostic needs.
3. Budget: Consider the cost of the kit, including any additional reagents or equipment that may be required. Ensure that the kit provides good value for money in terms of performance and reliability.
4. Usage Scenarios: Think about the specific applications for which you will use the kit, such as routine diagnostics, research, or specialized clinical tests. Ensure that the kit is suitable for your intended use.

Purchasing the α-Amylase Assay Kit requires careful consideration of product specifications, types, budget, and usage scenarios. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable and accurate results.

 Precautions

Proper handling and storage of the α-Amylase Assay Kit are essential to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature, typically between 2-8°C, to maintain the stability of the reagents. Avoid freezing or exposing the kit to high temperatures.
2. Handling: Always handle the reagents with care, using appropriate protective equipment such as gloves and lab coats. Avoid contamination by using sterile techniques.
3. Expiration Date: Check the expiration date before use. Do not use expired reagents, as they may not provide accurate results.
4. Calibration: Regularly calibrate the equipment used with the kit, such as spectrophotometers or microplate readers, to ensure accurate measurements.
5. Disposal: Dispose of used reagents and samples according to local regulations and laboratory protocols to prevent environmental contamination.

Proper storage, handling, and disposal of the α-Amylase Assay Kit are crucial for maintaining its effectiveness and ensuring accurate results. By following these precautions, you can maximize the kit's performance and reliability in clinical diagnostics.

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