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25-Hydroxy Vitamin D Assay Kit (HPLC-MS/MS)
The kit is composed of extraction solutions (A and C), derivatization solution, termination solution, reconstitution solution, mobile phases (A and B), internal standards (25-OH VD2 IS, 25-OH VD3 IS), calibrators (25-OH VD2, 25-OH VD3), quality controls (I and II), calibrator diluent, 96-well extraction plates, 96-well collection plates, and silicone cover mats. These components are designed to ensure accurate and reliable quantification of 25-hydroxy vitamin D in human plasma.
Product English Name:
25-Hydroxy Vitamin D Assay Kit (HPLC-MS/MS)
Alias:
Vitamin D Test Kit,25-OH Vitamin D Kit
Intended Use:
This kit is used for the quantitative in vitro determination of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 levels in human peripheral blood plasma. It is essential for assessing vitamin D status, which is crucial for bone health, immune function, and overall well-being.
Product Introduction

The 25-Hydroxy Vitamin D Assay Kit (HPLC-MS/MS) is a state-of-the-art diagnostic tool designed for the precise measurement of 25-hydroxy vitamin D levels in human plasma. Utilizing high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), this kit offers unparalleled accuracy and sensitivity. It is widely used in clinical laboratories to assess vitamin D status, which is vital for diagnosing deficiencies and monitoring treatment efficacy.

1. The kit features a robust extraction and derivatization process, ensuring high recovery rates and minimal interference from matrix effects. This makes it highly reliable for clinical diagnostics.
2. The composition includes specialized reagents and plates that streamline the workflow, reducing manual handling and potential errors. The inclusion of internal standards and quality controls further enhances the accuracy of the results.
3. Its primary use is in the quantification of 25-hydroxy vitamin D2 and D3, which are critical biomarkers for assessing vitamin D status. This is particularly important in populations at risk of deficiency, such as the elderly or those with limited sun exposure.

The 25-Hydroxy Vitamin D Assay Kit, also known as the Vitamin D Test Kit, is a comprehensive solution for measuring vitamin D levels. Its advanced technology and well-thought-out design make it a trusted tool in clinical settings, ensuring accurate and reliable results for patient care.

Main Features

The kit's main features include high sensitivity, precision, and ease of use, making it ideal for clinical laboratories. It employs HPLC-MS/MS technology for accurate quantification of 25-hydroxy vitamin D levels.

1. High sensitivity: The kit can detect low concentrations of 25-hydroxy vitamin D, making it suitable for diagnosing deficiencies.
2. Precision: The inclusion of internal standards and quality controls ensures reproducible and accurate results.
3. Ease of use: The kit's streamlined workflow reduces manual handling and minimizes the risk of errors.
4. Comprehensive components: All necessary reagents and plates are included, ensuring a complete solution for vitamin D testing.
5. Clinical relevance: The kit is widely used in clinical settings to assess vitamin D status, which is crucial for patient care.

The 25-Hydroxy Vitamin D Assay Kit stands out for its high sensitivity, precision, and user-friendly design. It is an indispensable tool for clinical laboratories, providing reliable results that are critical for patient diagnosis and treatment.

Clinical Applications

This kit is primarily used in clinical settings to assess vitamin D status, which is essential for diagnosing deficiencies and monitoring treatment efficacy. It is widely applied in hospitals, research institutions, and diagnostic laboratories.

1. Diagnosis of vitamin D deficiency: The kit is used to measure 25-hydroxy vitamin D levels, helping clinicians identify deficiencies.
2. Monitoring treatment: It is used to track the effectiveness of vitamin D supplementation in patients with deficiencies.
3. Research applications: The kit is also used in clinical studies to investigate the role of vitamin D in various health conditions.
4. Population screening: It is employed in large-scale screening programs to assess vitamin D status in at-risk populations.
5. Bone health assessment: The kit is used to evaluate vitamin D levels, which are crucial for maintaining bone health.

The 25-Hydroxy Vitamin D Assay Kit is a versatile tool in clinical practice, used for diagnosing deficiencies, monitoring treatment, and conducting research. Its applications are broad, making it a valuable asset in healthcare settings.

Purchasing Guide

When purchasing the 25-Hydroxy Vitamin D Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product specifications: Ensure the kit includes all necessary components and meets your laboratory's requirements for sensitivity and precision.
2. Product types: Choose between different kit formats based on your laboratory's workflow and sample volume.
3. Budget: Consider the cost of the kit and any additional reagents or equipment needed for its use.
4. Intended use: Select a kit that is suitable for your specific application, whether for clinical diagnostics, research, or population screening.

Purchasing the 25-Hydroxy Vitamin D Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By selecting the right kit, you can ensure accurate and reliable results for your laboratory's needs.

 Precautions

When using the 25-Hydroxy Vitamin D Assay Kit, it is important to follow the manufacturer's instructions carefully to ensure accurate results and avoid potential hazards.

1. Proper storage: Store the kit components at the recommended temperatures to maintain their stability and effectiveness.
2. Handling precautions: Use appropriate personal protective equipment when handling reagents to avoid exposure.
3. Calibration: Regularly calibrate the HPLC-MS/MS instrument to ensure accurate quantification of 25-hydroxy vitamin D.
4. Sample preparation: Follow the recommended procedures for sample preparation to avoid contamination or degradation.
5. Disposal: Dispose of used reagents and plates according to local regulations to minimize environmental impact.

Using the 25-Hydroxy Vitamin D Assay Kit requires attention to storage, handling, calibration, sample preparation, and disposal. By following these precautions, you can ensure the accuracy of your results and maintain a safe working environment.

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