The BD FACSCount Reagent Kit is a specialized tool designed for the precise enumeration of T lymphocyte subsets using flow cytometry. It is widely used in clinical and research settings to assess immune function by measuring the absolute counts of CD3, CD4, and CD8 T cells. The kit includes pre-mixed reagents and a user-friendly protocol, making it suitable for laboratories of varying expertise levels.

1. The BD FACSCount Reagent Kit is characterized by its high accuracy and reproducibility in T lymphocyte subset counting. It utilizes fluorescently labeled antibodies to specifically bind to CD3, CD4, and CD8 markers, enabling precise identification and quantification of these cell populations.
2. The kit consists of two main reagent tubes: one for CD4 and CD3 detection (green cap) and another for CD8 and CD3 detection (clear cap). Additionally, it includes phosphate-buffered saline with 5% formaldehyde for sample fixation, ensuring stable and reliable results.
3. This kit is primarily used in clinical diagnostics to monitor immune status, particularly in patients with HIV/AIDS, where CD4 T cell counts are critical for disease management. It is also valuable in research settings for studying immune responses in various diseases.

The BD FACSCount Reagent Kit, also known as the T Cell Subset Counting Kit, is a reliable and efficient tool for T lymphocyte subset enumeration. Its well-designed components and straightforward protocol make it a preferred choice for both clinical and research applications, particularly in immune monitoring.

The BD FACSCount Reagent Kit offers high precision in T lymphocyte subset counting, with pre-mixed reagents and a streamlined protocol for ease of use. It is compatible with the BD FACSCount™ system, ensuring accurate and reproducible results.

1. The kit provides highly accurate absolute counts of CD3, CD4, and CD8 T lymphocytes, essential for immune monitoring in clinical settings.
2. Pre-mixed reagents reduce preparation time and minimize user error, ensuring consistent and reliable results.
3. The inclusion of phosphate-buffered saline with 5% formaldehyde ensures sample stability, preserving cell integrity for accurate analysis.
4. The kit is designed for compatibility with the BD FACSCount™ system, offering seamless integration and user-friendly operation.
5. It is suitable for both clinical diagnostics and research applications, making it a versatile tool for immune system analysis.

The BD FACSCount Reagent Kit stands out for its precision, ease of use, and compatibility with the BD FACSCount™ system. Its pre-mixed reagents and stable sample fixation solution make it an indispensable tool for accurate T lymphocyte subset counting.

The BD FACSCount Reagent Kit is widely used in clinical settings for immune monitoring, particularly in HIV/AIDS management, autoimmune diseases, and post-transplant immune reconstitution.

1. In HIV/AIDS management, the kit is used to monitor CD4 T cell counts, a critical parameter for assessing disease progression and treatment efficacy.
2. It is employed in autoimmune disease research to study changes in T lymphocyte subsets, providing insights into disease mechanisms and therapeutic responses.
3. The kit is used in post-transplant settings to monitor immune reconstitution and detect potential graft-versus-host disease.
4. It is also utilized in cancer immunotherapy research to evaluate the impact of treatments on T cell populations.
5. In general immunology research, the kit aids in understanding the role of T lymphocytes in various diseases and immune responses.

The BD FACSCount Reagent Kit is a versatile tool in clinical and research settings, particularly for immune monitoring in HIV/AIDS, autoimmune diseases, and post-transplant care. Its ability to provide precise T lymphocyte subset counts makes it invaluable for disease management and research.

When purchasing the BD FACSCount Reagent Kit, consider factors such as product specifications, compatibility with your laboratory equipment, budget, and intended applications.

1. Product parameters: Ensure the kit includes all necessary components, such as CD4/CD3 and CD8/CD3 reagent tubes, and phosphate-buffered saline with 5% formaldehyde.
2. Product types: Choose the kit that best suits your laboratory's needs, whether for clinical diagnostics or research purposes.
3. Budget considerations: Evaluate the cost-effectiveness of the kit, considering its accuracy, ease of use, and compatibility with existing equipment.
4. Usage scenarios: Determine the kit's suitability for your specific applications, such as HIV/AIDS monitoring, autoimmune disease research, or post-transplant immune reconstitution.

Purchasing the BD FACSCount Reagent Kit requires careful consideration of product specifications, compatibility, budget, and intended use. Its versatility and reliability make it a valuable addition to any laboratory focused on immune monitoring.

Proper handling and storage of the BD FACSCount Reagent Kit are essential to ensure accurate results and maintain reagent stability.

1. Store the kit at the recommended temperature (2-8°C) and protect it from light to maintain reagent stability.
2. Follow the manufacturer's instructions for sample preparation and reagent handling to avoid contamination and ensure accurate results.
3. Use appropriate personal protective equipment (PPE) when handling reagents, especially formaldehyde-containing solutions.
4. Ensure the BD FACSCount™ system is properly calibrated and maintained to achieve reliable and reproducible results.
5. Dispose of reagents and samples according to local regulations and laboratory safety protocols.

The BD FACSCount Reagent Kit requires careful handling and storage to ensure optimal performance. Adhering to safety protocols and manufacturer guidelines is crucial for accurate and reliable T lymphocyte subset counting.