The 25-Hydroxy Vitamin D3 Quantitative Assay Kit is a highly sensitive and specific tool for measuring 25(OH)D3 levels in human serum. Utilizing the enzyme-linked immunosorbent assay (ELISA) method, this kit provides accurate and reproducible results, making it an essential tool for both clinical diagnostics and research. The kit includes all necessary components, such as enzyme-labeled plates, enzyme conjugates, calibrators, and chromogenic solutions, ensuring a streamlined and efficient assay process.

1. The kit features a highly specific monoclonal antibody against 25(OH)D3, ensuring minimal cross-reactivity and high accuracy in detecting 25(OH)D3 levels. The enzyme-labeled plates are pre-coated with this antibody, providing a stable and reliable platform for the assay.
2. The kit includes a series of calibrators (S0-S5) with known concentrations of 25(OH)D3, allowing for the generation of a standard curve. This curve is essential for quantifying the 25(OH)D3 levels in unknown samples. The calibrators are prepared in a matrix that mimics human serum, ensuring accurate and consistent results.
3. The kit is designed for ease of use, with all components provided in ready-to-use formats. The chromogenic solutions (A and B) are optimized for rapid and sensitive detection of 25(OH)D3, while the concentrated wash buffer ensures thorough removal of unbound materials, reducing background noise and improving assay precision.

The 25-Hydroxy Vitamin D3 Quantitative Assay Kit is a reliable and efficient tool for measuring 25(OH)D3 levels in human serum. Its high specificity, accuracy, and ease of use make it an invaluable resource for clinical diagnostics and research. Whether you're assessing vitamin D status in patients or conducting research on the role of vitamin D in health and disease, this kit provides the precision and reliability you need.

The 25-Hydroxy Vitamin D3 Quantitative Assay Kit offers high sensitivity, specificity, and reproducibility in measuring 25(OH)D3 levels. It is designed for both clinical and research applications, providing accurate results with minimal cross-reactivity.

1. The kit utilizes a highly specific monoclonal antibody against 25(OH)D3, ensuring accurate detection with minimal interference from other compounds. This specificity is crucial for obtaining reliable results in both clinical and research settings.
2. The kit includes a series of calibrators with known concentrations of 25(OH)D3, allowing for the generation of a standard curve. This curve is essential for quantifying 25(OH)D3 levels in unknown samples, ensuring accurate and consistent results.
3. The enzyme-labeled plates are pre-coated with the monoclonal antibody, providing a stable and reliable platform for the assay. This pre-coating eliminates the need for additional coating steps, simplifying the assay process and reducing the risk of errors.
4. The chromogenic solutions (A and B) are optimized for rapid and sensitive detection of 25(OH)D3. These solutions produce a color change that is proportional to the amount of 25(OH)D3 present in the sample, allowing for easy and accurate quantification.
5. The concentrated wash buffer ensures thorough removal of unbound materials, reducing background noise and improving assay precision. This step is critical for obtaining accurate and reproducible results, especially in low-concentration samples.

The 25-Hydroxy Vitamin D3 Quantitative Assay Kit is a powerful tool for accurately measuring 25(OH)D3 levels in human serum. Its high specificity, sensitivity, and ease of use make it an essential resource for both clinical diagnostics and research. Whether you're assessing vitamin D status in patients or conducting research on the role of vitamin D in health and disease, this kit provides the precision and reliability you need.

The 25-Hydroxy Vitamin D3 Quantitative Assay Kit is widely used in clinical diagnostics to assess vitamin D status, which is crucial for bone health, immune function, and overall well-being. It is also employed in research studies to investigate the role of vitamin D in various physiological and pathological conditions.

1. In clinical diagnostics, the kit is used to assess vitamin D status in patients, particularly those at risk of vitamin D deficiency or insufficiency. Accurate measurement of 25(OH)D3 levels is essential for diagnosing and managing conditions such as osteoporosis, rickets, and other bone disorders.
2. The kit is also used in research studies to investigate the role of vitamin D in various physiological processes, including calcium homeostasis, immune function, and cell proliferation. Accurate measurement of 25(OH)D3 levels is crucial for understanding the role of vitamin D in health and disease.
3. In epidemiological studies, the kit is used to assess the prevalence of vitamin D deficiency in different populations. This information is essential for developing public health strategies to address vitamin D deficiency and its associated health risks.
4. The kit is also used in clinical trials to evaluate the efficacy of vitamin D supplementation in improving vitamin D status and reducing the risk of vitamin D-related health conditions. Accurate measurement of 25(OH)D3 levels is crucial for assessing the effectiveness of these interventions.
5. In veterinary medicine, the kit is used to assess vitamin D status in animals, particularly those at risk of vitamin D deficiency or insufficiency. Accurate measurement of 25(OH)D3 levels is essential for diagnosing and managing conditions such as rickets and other bone disorders in animals.

The 25-Hydroxy Vitamin D3 Quantitative Assay Kit is a versatile tool for assessing vitamin D status in both clinical and research settings. Its high accuracy and reliability make it an essential resource for diagnosing and managing vitamin D-related conditions, as well as for investigating the role of vitamin D in health and disease.

When purchasing the 25-Hydroxy Vitamin D3 Quantitative Assay Kit, it is important to consider factors such as product specifications, intended use, budget, and application scenarios to ensure that the kit meets your specific needs.

1. Product specifications: The kit is available in two formats: 48 tests per box and 96 tests per box. Consider the volume of testing you will be conducting to determine which format is most suitable for your needs.
2. Product type: The kit is designed for the quantitative measurement of 25(OH)D3 levels in human serum. Ensure that the kit is compatible with your sample type and intended use.
3. Budget: The cost of the kit may vary depending on the format and supplier. Consider your budget and the cost per test when selecting the kit.
4. Application scenarios: The kit is suitable for both clinical diagnostics and research applications. Consider the specific requirements of your application, such as sensitivity, specificity, and ease of use, when selecting the kit.

When purchasing the 25-Hydroxy Vitamin D3 Quantitative Assay Kit, it is important to carefully consider your specific needs and requirements. By taking into account factors such as product specifications, intended use, budget, and application scenarios, you can ensure that the kit you select will meet your needs and provide accurate and reliable results.

When using the 25-Hydroxy Vitamin D3 Quantitative Assay Kit, it is important to follow the manufacturer's instructions carefully to ensure accurate and reliable results. Proper handling, storage, and disposal of the kit components are also essential to maintain the integrity of the assay.

1. Always follow the manufacturer's instructions for handling and storing the kit components. Improper storage or handling can affect the performance of the assay and lead to inaccurate results.
2. Ensure that all reagents are at room temperature before use. Cold reagents can affect the performance of the assay and lead to inaccurate results.
3. Use only the provided calibrators and controls to generate the standard curve and validate the assay. Using non-standardized materials can lead to inaccurate results.
4. Avoid cross-contamination between samples and reagents. Use separate pipette tips for each sample and reagent to prevent contamination and ensure accurate results.
5. Dispose of all kit components and waste materials according to local regulations and guidelines. Improper disposal can pose a risk to human health and the environment.

The 25-Hydroxy Vitamin D3 Quantitative Assay Kit is a powerful tool for accurately measuring 25(OH)D3 levels in human serum. However, it is important to follow the manufacturer's instructions carefully and take appropriate precautions to ensure accurate and reliable results. By handling, storing, and disposing of the kit components properly, you can maintain the integrity of the assay and obtain accurate and reliable results.