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N-Acetyl-β-D-Glucosaminidase Assay Kit (CNP-NAG Substrate Method)
The N-Acetyl-β-D-Glucosaminidase Assay Kit is composed of two main reagents: R1, which contains a modified Tris-citrate buffer at pH 5.0, and R2, which includes CNP-NAG at a concentration of 3.5 mmol/L. This kit is designed for the quantitative determination of N-Acetyl-β-D-Glucosaminidase activity in human urine or serum.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (CNP-NAG Substrate Method)
Alias:
NAG Assay Kit,CNP-NAG Kit
Intended Use:
This kit is used to quantitatively measure the activity of N-Acetyl-β-D-Glucosaminidase in human urine or serum. It is particularly useful in clinical settings for diagnosing and monitoring conditions related to kidney function and other metabolic disorders.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a specialized diagnostic tool designed for the accurate measurement of NAG enzyme activity in biological samples. This enzyme is a key biomarker for renal and metabolic health, making the kit essential for clinical diagnostics.

1. The kit features a highly sensitive and specific substrate method, ensuring precise measurement of NAG activity. The use of CNP-NAG as a substrate allows for clear and reliable results.
2. The composition includes a modified Tris-citrate buffer (R1) and CNP-NAG (R2), which together create an optimal environment for the enzymatic reaction. The buffer maintains a stable pH, while the substrate provides a consistent reaction medium.
3. The primary use of this kit is in clinical laboratories for the quantitative analysis of NAG activity in urine or serum. It aids in the diagnosis and monitoring of kidney-related diseases and other metabolic conditions.

The N-Acetyl-β-D-Glucosaminidase Assay Kit, also known as the NAG Assay Kit, is a vital tool in clinical diagnostics. Its precise measurement capabilities and reliable composition make it indispensable for assessing renal and metabolic health.

Main Features

The main features of this kit include high sensitivity, specificity, and ease of use, making it a preferred choice for clinical laboratories.

1. High Sensitivity: The kit is designed to detect even low levels of NAG activity, ensuring accurate results in various clinical conditions.
2. Specificity: The use of CNP-NAG as a substrate ensures that the assay is specific to NAG, reducing the risk of cross-reactivity with other enzymes.
3. Ease of Use: The kit comes with clear instructions and requires minimal preparation, making it user-friendly for laboratory technicians.
4. Stability: The reagents are stable under recommended storage conditions, ensuring consistent performance over time.
5. Versatility: The kit can be used with both urine and serum samples, providing flexibility in clinical applications.

The N-Acetyl-β-D-Glucosaminidase Assay Kit stands out for its sensitivity, specificity, and ease of use. These features make it a reliable and versatile tool for clinical diagnostics, particularly in the assessment of renal and metabolic health.

Clinical Applications

This kit is widely used in clinical settings for the diagnosis and monitoring of kidney diseases and other metabolic disorders.

1. Kidney Disease Diagnosis: The kit is used to measure NAG activity in urine, which is a key indicator of renal tubular damage.
2. Monitoring Treatment: It helps in monitoring the effectiveness of treatments for kidney diseases by tracking changes in NAG activity over time.
3. Metabolic Disorders: The kit is also used in the diagnosis of metabolic disorders where NAG activity is altered.
4. Research Applications: Beyond clinical diagnostics, the kit is used in research studies to understand the role of NAG in various physiological and pathological processes.
5. Preventive Screening: It is employed in preventive screening programs to detect early signs of kidney dysfunction in at-risk populations.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a cornerstone in clinical diagnostics, particularly for kidney diseases and metabolic disorders. Its applications extend from diagnosis and treatment monitoring to research and preventive screening, making it an invaluable tool in healthcare.

Purchasing Guide

This guide provides essential information for purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, including product specifications, types, budget considerations, and usage scenarios.

1. Product Specifications: The kit includes R1 (modified Tris-citrate buffer) and R2 (CNP-NAG substrate), with specific concentrations and pH levels optimized for accurate NAG activity measurement.
2. Product Types: Available in different sizes and formats to suit various laboratory needs, from small-scale research to high-throughput clinical labs.
3. Budget Considerations: The cost of the kit varies based on the quantity and format, with bulk purchases often offering cost savings.
4. Usage Scenarios: The kit is suitable for use in clinical laboratories, research institutions, and preventive health screening programs.

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, it's important to consider product specifications, types, budget, and intended usage scenarios. This ensures that the kit meets the specific needs of your laboratory or clinical setting.

 Precautions

Proper handling and storage of the N-Acetyl-β-D-Glucosaminidase Assay Kit are crucial to ensure accurate results and maintain reagent stability.

1. Storage Conditions: Store the kit at the recommended temperature to maintain reagent stability and performance.
2. Handling: Follow proper laboratory protocols when handling the reagents to avoid contamination and ensure accurate results.
3. Expiration Date: Always check the expiration date before use to ensure the reagents are still effective.
4. Sample Preparation: Ensure that urine or serum samples are properly collected and prepared according to the kit's instructions.
5. Disposal: Dispose of used reagents and samples in accordance with local regulations and laboratory safety guidelines.

To get the most out of the N-Acetyl-β-D-Glucosaminidase Assay Kit, it's essential to follow proper storage, handling, and disposal procedures. These precautions help maintain the integrity of the reagents and ensure accurate and reliable results in clinical diagnostics.

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