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N-Acetyl-beta-D-Glucosaminidase (NAG) Assay Kit (MPT Method)
The NAG Assay Kit is composed of Reagent 1 (R1), Reagent 2 (R2), optional control materials, and optional calibrators. R1 contains a citric acid buffer (pH 4.5, 200 mmol/L), while R2 includes 6-methyl-2-pyridyl-N-acetyl-1-thio-beta-D-glucosaminide at a concentration of 6 mg/dL. The calibrator is a single-level lyophilized product containing NAG, phosphate buffer (50 mM), lactose (5%), and sodium azide (1%), with a target concentration range of 100-200 IU/L. The control material is also a single-level lyophilized product with similar components and a target concentration range of 5-25 IU/L.
Product English Name:
N-Acetyl-beta-D-Glucosaminidase (NAG) Assay Kit (MPT Method)
Alias:
NAG Test Kit,NAG Assay Kit
Intended Use:
This product is designed for the in vitro quantitative determination of N-acetyl-beta-D-glucosaminidase (NAG) levels in human urine. It is widely used in clinical diagnostics to assess renal function and detect early kidney damage.
Product Introduction

The N-Acetyl-beta-D-Glucosaminidase (NAG) Assay Kit is a specialized diagnostic tool used to measure the activity of NAG in human urine. NAG is an enzyme found in the lysosomes of renal tubular cells, and its increased levels in urine can indicate kidney damage or dysfunction. This kit employs the MPT (Methylumbelliferyl Phosphate) method, which is known for its high sensitivity and specificity. The kit includes all necessary reagents and optional calibrators and controls to ensure accurate and reliable results.

1. The NAG Assay Kit is characterized by its high sensitivity and specificity, making it an excellent tool for detecting early renal damage. The MPT method used in this kit ensures precise measurement of NAG activity, which is crucial for accurate diagnosis.
2. The kit is composed of two main reagents: Reagent 1 (R1) and Reagent 2 (R2). R1 contains a citric acid buffer, while R2 includes a specific substrate for NAG. Additionally, the kit offers optional calibrators and controls to ensure the accuracy of the results.
3. The primary use of this kit is to quantitatively determine NAG levels in human urine. Elevated NAG levels are often associated with renal tubular damage, making this kit an essential tool in nephrology and clinical diagnostics.

The NAG Assay Kit is a reliable and sensitive tool for measuring NAG activity in human urine. Its high specificity and the inclusion of optional calibrators and controls make it a valuable asset in clinical diagnostics, particularly in the assessment of renal function.

Main Features

The NAG Assay Kit offers high sensitivity and specificity for detecting NAG activity in human urine. It includes all necessary reagents and optional controls for accurate measurement.

1. The kit uses the MPT method, which is highly sensitive and specific for detecting NAG activity. This method ensures precise and reliable results, making it ideal for clinical diagnostics.
2. The kit includes Reagent 1 (R1) and Reagent 2 (R2), which are essential for the assay. R1 contains a citric acid buffer, while R2 includes a specific substrate for NAG.
3. Optional calibrators and controls are provided to ensure the accuracy of the results. These components are crucial for validating the assay and ensuring consistent performance.
4. The kit is designed for ease of use, with clear instructions and a straightforward protocol. This makes it accessible to both experienced and novice users.
5. The NAG Assay Kit is widely used in clinical settings to assess renal function and detect early kidney damage. Its reliability and accuracy make it a trusted tool in nephrology.

The NAG Assay Kit is a comprehensive and reliable tool for measuring NAG activity in human urine. Its high sensitivity, specificity, and ease of use make it an essential component of clinical diagnostics, particularly in the field of nephrology.

Clinical Applications

The NAG Assay Kit is primarily used in clinical settings to assess renal function and detect early kidney damage. It is a valuable tool in nephrology and clinical diagnostics.

1. The kit is used to measure NAG activity in human urine, which is a marker for renal tubular damage. Elevated NAG levels can indicate early kidney dysfunction, making this kit essential for early diagnosis.
2. In clinical practice, the NAG Assay Kit is used to monitor patients with known kidney diseases, such as diabetic nephropathy or glomerulonephritis. Regular monitoring helps in assessing disease progression and treatment efficacy.
3. The kit is also used in research settings to study the effects of various drugs and treatments on renal function. Its high sensitivity and specificity make it a reliable tool for such studies.
4. In addition to clinical diagnostics, the NAG Assay Kit is used in epidemiological studies to assess the prevalence of kidney damage in specific populations. This helps in understanding the burden of kidney diseases and planning public health interventions.
5. The kit is also used in occupational health settings to monitor workers exposed to nephrotoxic substances. Early detection of kidney damage can help in preventing further damage and ensuring worker safety.

The NAG Assay Kit is a versatile tool used in various clinical and research settings to assess renal function and detect early kidney damage. Its reliability and accuracy make it a trusted choice for healthcare professionals and researchers alike.

Purchasing Guide

When purchasing the NAG Assay Kit, it is important to consider product specifications, types, budget, and intended use to ensure it meets your needs.

1. Product specifications: Ensure that the kit includes all necessary reagents, such as Reagent 1 (R1) and Reagent 2 (R2), as well as optional calibrators and controls. Verify the concentration ranges and compatibility with your laboratory equipment.
2. Product types: The NAG Assay Kit is available in different configurations, including kits with or without calibrators and controls. Choose the type that best suits your laboratory's needs and budget.
3. Budget: Consider the cost of the kit, including any additional expenses for calibrators, controls, and shipping. Ensure that the kit fits within your laboratory's budget without compromising on quality.
4. Intended use: Determine the primary use of the kit, whether for clinical diagnostics, research, or occupational health. Ensure that the kit's sensitivity, specificity, and ease of use align with your intended application.

Purchasing the NAG Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can ensure that the kit meets your laboratory's needs and provides reliable results.

 Precautions

When using the NAG Assay Kit, it is important to follow all instructions carefully to ensure accurate and reliable results. Proper handling and storage of reagents are crucial.

1. Always follow the manufacturer's instructions for handling and storing the reagents. Improper storage can affect the stability and performance of the reagents, leading to inaccurate results.
2. Ensure that all equipment used in the assay is properly calibrated and maintained. Any deviation from the recommended procedures can compromise the accuracy of the results.
3. Use the optional calibrators and controls provided with the kit to validate the assay. This helps in ensuring the consistency and reliability of the results.
4. Handle all reagents with care, wearing appropriate personal protective equipment (PPE) such as gloves and lab coats. Some reagents may contain hazardous substances.
5. Dispose of all waste materials according to local regulations and guidelines. Proper disposal is essential to prevent environmental contamination and ensure safety.

Using the NAG Assay Kit requires careful attention to handling, storage, and disposal of reagents. By following the manufacturer's instructions and taking necessary precautions, you can ensure accurate and reliable results while maintaining safety in the laboratory.

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