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N-Acetyl-β-D-Glucosaminidase (NAG) Assay Kit (MPT Substrate Method)
The N-Acetyl-β-D-Glucosaminidase (NAG) Assay Kit is designed for the quantitative determination of NAG activity in human serum or urine. The kit includes two main reagents: Reagent 1 (R1), a citrate buffer solution with a pH of 4.4 and a concentration of 60 mmol/L, and Reagent 2 (R2), which contains 6-Methyl-2-Thiopyridine-N-Acetyl-β-D-Glucosaminide (MPT-NAG) at a concentration of 6.34 g/L. These components work together to facilitate the accurate measurement of NAG activity, which is crucial for diagnosing and monitoring various medical conditions.
Product English Name:
N-Acetyl-β-D-Glucosaminidase (NAG) Assay Kit (MPT Substrate Method)
Alias:
NAG Test Kit,MPT-NAG Assay Kit
Intended Use:
The N-Acetyl-β-D-Glucosaminidase (NAG) Assay Kit is intended for the in vitro quantitative determination of NAG activity in human serum or urine. This assay is particularly useful in clinical settings for diagnosing and monitoring conditions related to kidney function, as NAG is a sensitive marker for renal tubular damage. The kit provides a reliable and precise method for measuring NAG levels, aiding in the early detection and management of renal diseases.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase (NAG) Assay Kit is a specialized diagnostic tool used to measure the activity of the enzyme NAG in human serum or urine. NAG is an enzyme that plays a critical role in the breakdown of complex carbohydrates and is often used as a biomarker for renal tubular damage. This kit employs the MPT substrate method, which offers high sensitivity and specificity for NAG detection. The kit includes two main reagents: a citrate buffer and a substrate solution, which together enable accurate and reproducible results. This assay is essential for clinicians and researchers who need to assess kidney function and monitor the progression of renal diseases.

1. The NAG Assay Kit is characterized by its high sensitivity and specificity, making it an ideal choice for detecting low levels of NAG activity in biological samples. The MPT substrate method ensures that the assay is both accurate and reproducible, providing reliable results for clinical and research applications.
2. The kit consists of two main components: Reagent 1 (R1), a citrate buffer solution that maintains the optimal pH for the enzymatic reaction, and Reagent 2 (R2), which contains the MPT-NAG substrate. These reagents are carefully formulated to ensure stability and performance, allowing for consistent and accurate measurements of NAG activity.
3. The primary use of the NAG Assay Kit is for the quantitative determination of NAG activity in human serum or urine. This assay is particularly valuable in clinical settings for diagnosing and monitoring renal tubular damage, as NAG is a sensitive marker for this condition. The kit provides clinicians with a reliable tool for assessing kidney function and managing renal diseases.

The N-Acetyl-β-D-Glucosaminidase (NAG) Assay Kit, also known as the MPT-NAG Assay Kit, is a vital diagnostic tool for measuring NAG activity in human serum or urine. Its high sensitivity and specificity, combined with the reliable MPT substrate method, make it an essential resource for clinicians and researchers. The kit's two main reagents work in harmony to provide accurate and reproducible results, aiding in the diagnosis and management of renal diseases. This kit is a testament to the advancements in diagnostic technology, offering a precise and efficient solution for monitoring kidney health.

Main Features

The NAG Assay Kit is designed for the precise measurement of NAG activity in biological samples. It offers high sensitivity, specificity, and reproducibility, making it a reliable tool for clinical diagnostics and research.

1. The kit employs the MPT substrate method, which provides high sensitivity and specificity for detecting NAG activity. This method ensures that even low levels of NAG can be accurately measured, making it suitable for early detection of renal tubular damage.
2. The assay is highly reproducible, with consistent results across different batches and operators. This reliability is crucial for clinical diagnostics, where accurate and consistent results are essential for patient care.
3. The kit includes two main reagents: a citrate buffer and a substrate solution. These reagents are carefully formulated to ensure stability and performance, allowing for accurate measurements of NAG activity over time.
4. The assay is easy to perform, with a straightforward protocol that can be completed in a short amount of time. This ease of use makes it accessible to a wide range of users, from clinical laboratories to research institutions.
5. The NAG Assay Kit is versatile, suitable for use with both serum and urine samples. This flexibility allows clinicians to choose the most appropriate sample type for their specific diagnostic needs.

The NAG Assay Kit stands out for its high sensitivity, specificity, and reproducibility, making it a reliable tool for measuring NAG activity in biological samples. Its ease of use and versatility further enhance its appeal, making it a valuable resource for clinicians and researchers alike. The kit's robust performance and consistent results ensure that it remains a trusted choice for diagnosing and monitoring renal diseases.

Clinical Applications

The NAG Assay Kit is widely used in clinical settings for the diagnosis and monitoring of renal tubular damage. Its high sensitivity and specificity make it an essential tool for assessing kidney function and managing renal diseases.

1. The kit is commonly used in clinical laboratories to measure NAG activity in serum or urine samples. This measurement is crucial for diagnosing renal tubular damage, as NAG is a sensitive marker for this condition.
2. The assay is also used in research settings to study the role of NAG in various physiological and pathological processes. Researchers can use the kit to investigate the mechanisms underlying renal diseases and develop new therapeutic strategies.
3. In addition to its diagnostic applications, the NAG Assay Kit is used in monitoring the progression of renal diseases. Regular measurements of NAG activity can help clinicians assess the effectiveness of treatment and make informed decisions about patient care.
4. The kit is also valuable in epidemiological studies, where it can be used to assess the prevalence of renal tubular damage in different populations. This information can inform public health strategies and improve the management of renal diseases on a larger scale.
5. Finally, the NAG Assay Kit is used in drug development, where it can be employed to evaluate the efficacy of new drugs in treating renal diseases. The kit's high sensitivity and specificity make it an ideal tool for assessing the impact of therapeutic interventions on NAG activity.

The NAG Assay Kit is a versatile and essential tool in both clinical and research settings. Its ability to accurately measure NAG activity in serum or urine samples makes it invaluable for diagnosing and monitoring renal tubular damage. Whether used in clinical laboratories, research institutions, or drug development, the kit provides reliable and reproducible results that support the effective management of renal diseases. Its widespread application underscores its importance in advancing our understanding and treatment of kidney-related conditions.

Purchasing Guide

When purchasing the NAG Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product specifications: Ensure that the kit meets your specific requirements, including sensitivity, specificity, and reproducibility. Check the concentration and stability of the reagents to ensure they are suitable for your intended use.
2. Product types: The NAG Assay Kit is available in different formats, including single-use and bulk options. Choose the type that best fits your laboratory's needs and workflow.
3. Budget: Consider the cost of the kit, including any additional expenses such as shipping and handling. Compare prices from different suppliers to find the best value for your budget.
4. Intended use: Determine the primary application of the kit, whether for clinical diagnostics, research, or drug development. Ensure that the kit is compatible with your specific sample types and analytical methods.

Purchasing the NAG Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking the time to evaluate these aspects, you can select a kit that meets your specific needs and ensures accurate and reliable results. Whether you are a clinician, researcher, or drug developer, this guide will help you make an informed decision and choose the best NAG Assay Kit for your application.

 Precautions

When using the NAG Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate and reliable results. This section outlines key precautions to consider.

1. Storage: Store the kit at the recommended temperature to maintain the stability and performance of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Use aseptic techniques when handling the reagents to prevent contamination. Ensure that all equipment and surfaces are clean and free from contaminants.
3. Sample preparation: Follow the recommended procedures for preparing serum or urine samples. Ensure that samples are properly collected, stored, and processed to avoid degradation or interference.
4. Calibration: Regularly calibrate the assay using the provided standards to ensure accurate and consistent results. Follow the manufacturer's instructions for calibration and quality control.
5. Disposal: Dispose of used reagents and samples according to local regulations and guidelines. Ensure that all waste is properly labeled and handled to prevent environmental contamination.

Proper handling and storage of the NAG Assay Kit are essential for obtaining accurate and reliable results. By following the recommended precautions, you can ensure the stability and performance of the reagents, prevent contamination, and maintain the integrity of your samples. These precautions are crucial for achieving consistent and reproducible results, whether in clinical diagnostics, research, or drug development. Taking these steps will help you maximize the effectiveness of the NAG Assay Kit and ensure the success of your experiments or diagnostic procedures.

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