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N-Acetyl-β-D-Glucosaminidase Assay Kit (Enzymatic Colorimetric Method)
The N-Acetyl-β-D-Glucosaminidase Assay Kit is composed of two main reagents: R1 and R2. R1 contains a buffer solution with a concentration of 100-500 mmol/L, a surfactant at 0.1-3.0%, and a preservative at 0.10-0.50%. R2 includes 6-Methyl-2-thiopyridine-N-acetyl-β-D-glucosaminide (MPT-NAG) at a concentration of 20-100 mmol/L. The kit also includes a calibrator and a control, both containing bovine serum albumin (2%-10%), N-Acetyl-β-D-Glucosaminidase (30-100 U/L for calibrator and 30-150 U/L for control), and a preservative (0.10-0.50%).
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (Enzymatic Colorimetric Method)
Alias:
NAG Assay Kit,Glucosaminidase Test Kit
Intended Use:
This kit is designed for the quantitative in vitro determination of N-Acetyl-β-D-Glucosaminidase activity in human urine. It is primarily used in clinical settings to assist in the evaluation of renal tubular damage, providing crucial information for diagnosing and monitoring kidney-related conditions.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a specialized diagnostic tool used to measure the activity of the enzyme N-Acetyl-β-D-Glucosaminidase in human urine. This enzyme is a marker for renal tubular damage, making the kit an essential component in the assessment of kidney health. The kit employs an enzymatic colorimetric method, ensuring accurate and reliable results. It is widely used in clinical laboratories and research institutions for its precision and ease of use.

1. The kit features a highly sensitive enzymatic colorimetric method, which allows for the precise measurement of N-Acetyl-β-D-Glucosaminidase activity. This method is known for its accuracy and reliability, making it a preferred choice in clinical diagnostics.
2. The composition of the kit includes two main reagents, R1 and R2, along with a calibrator and a control. R1 contains a buffer solution, surfactant, and preservative, while R2 includes the substrate MPT-NAG. The calibrator and control are essential for ensuring the accuracy of the assay.
3. The primary use of this kit is to quantitatively determine the activity of N-Acetyl-β-D-Glucosaminidase in human urine. This measurement is crucial for assessing renal tubular damage, aiding in the diagnosis and monitoring of kidney-related conditions.

The N-Acetyl-β-D-Glucosaminidase Assay Kit, also known as the NAG Assay Kit, is a vital tool in clinical diagnostics. Its ability to accurately measure enzyme activity in urine makes it indispensable for evaluating kidney health. The kit's composition, featuring precise reagents and controls, ensures reliable results, making it a trusted choice for healthcare professionals.

Main Features

The N-Acetyl-β-D-Glucosaminidase Assay Kit offers a range of features designed to ensure accurate and reliable measurement of enzyme activity. Its enzymatic colorimetric method is highly sensitive, and the inclusion of calibrators and controls enhances the precision of the assay.

1. The kit utilizes an enzymatic colorimetric method, which is known for its high sensitivity and accuracy in measuring enzyme activity.
2. It includes two main reagents, R1 and R2, which are essential for the assay. R1 contains a buffer solution, surfactant, and preservative, while R2 includes the substrate MPT-NAG.
3. The kit comes with a calibrator and a control, both of which are crucial for ensuring the accuracy and reliability of the assay results.
4. The assay is designed for the quantitative determination of N-Acetyl-β-D-Glucosaminidase activity in human urine, providing valuable information for the assessment of renal tubular damage.
5. The kit is easy to use and is suitable for both clinical laboratories and research institutions, making it a versatile tool in the field of diagnostics.

The N-Acetyl-β-D-Glucosaminidase Assay Kit stands out for its precision and reliability. Its enzymatic colorimetric method, combined with carefully formulated reagents and controls, ensures accurate measurement of enzyme activity. This makes it an essential tool for diagnosing and monitoring kidney-related conditions.

Clinical Applications

The N-Acetyl-β-D-Glucosaminidase Assay Kit is primarily used in clinical settings to assess renal tubular damage. Its ability to measure enzyme activity in urine makes it a valuable tool for diagnosing and monitoring kidney-related conditions.

1. The kit is used in clinical laboratories to quantitatively determine the activity of N-Acetyl-β-D-Glucosaminidase in human urine, aiding in the diagnosis of renal tubular damage.
2. It is particularly useful in monitoring patients with known kidney conditions, providing crucial information on the progression of the disease.
3. The assay is also employed in research settings to study the effects of various treatments on renal function, offering insights into potential therapeutic interventions.
4. In addition to its diagnostic applications, the kit is used in epidemiological studies to assess the prevalence of kidney-related conditions in different populations.
5. The kit's ease of use and reliability make it a preferred choice for healthcare professionals in both clinical and research settings.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a versatile tool in clinical diagnostics. Its ability to accurately measure enzyme activity in urine makes it indispensable for assessing renal tubular damage. Whether used in clinical laboratories or research institutions, the kit provides valuable insights into kidney health, aiding in the diagnosis and monitoring of kidney-related conditions.

Purchasing Guide

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product specifications: Ensure that the kit meets your laboratory's requirements, including the sensitivity and accuracy of the assay.
2. Product types: Consider the different types of kits available, such as those with varying reagent concentrations or additional controls, to find the one that best suits your needs.
3. Budget: Determine your budget and compare prices from different suppliers to find a kit that offers the best value for money.
4. Intended use: Consider the specific applications for which you will be using the kit, such as clinical diagnostics or research, to ensure that it meets your requirements.

Purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you select the right kit for your needs, whether for clinical diagnostics or research purposes.

 Precautions

When using the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to follow certain precautions to ensure accurate results and maintain safety in the laboratory.

1. Always wear appropriate personal protective equipment (PPE), such as gloves and lab coats, when handling the reagents to avoid contamination and ensure safety.
2. Ensure that all reagents are stored at the recommended temperatures to maintain their stability and effectiveness.
3. Follow the manufacturer's instructions carefully when preparing and using the reagents to ensure accurate and reliable results.
4. Dispose of all waste materials according to local regulations and laboratory protocols to prevent environmental contamination.
5. Regularly calibrate and maintain laboratory equipment, such as pipettes and microplate readers, to ensure the accuracy of the assay.

Using the N-Acetyl-β-D-Glucosaminidase Assay Kit requires adherence to specific precautions to ensure accurate results and maintain laboratory safety. By following these guidelines, you can minimize the risk of contamination, ensure the stability of reagents, and achieve reliable outcomes in your diagnostic or research work.

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