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N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method)
The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) is composed of two main reagents: Reagent 1 (R1) contains a citrate-phosphate buffer (pH 4.6, 200 mmol/L), and Reagent 2 (R2) contains the same buffer with MPT-NAG (7.0 g/L). The kit also includes a calibrator (lyophilized) with sodium chloride (9.0 g/L), bovine serum albumin (10.0 g/L), and human-derived N-Acetyl-β-D-Glucosaminidase (60.0±10.0 U/L). Additionally, there are two quality control samples (lyophilized) with varying concentrations of N-Acetyl-β-D-Glucosaminidase.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method)
Alias:
NAG Assay Kit,MPT-NAG Kit
Intended Use:
This kit is designed for the in vitro quantitative determination of N-Acetyl-β-D-Glucosaminidase (NAG) content in human urine. It is widely used in clinical laboratories to assess kidney function and detect renal tubular damage.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) is a specialized diagnostic tool used to measure the activity of NAG in human urine. NAG is an enzyme that is primarily found in the lysosomes of renal tubular cells, and its presence in urine can indicate renal tubular damage. This kit provides a reliable and accurate method for quantifying NAG levels, which is crucial for diagnosing and monitoring kidney-related diseases.

1. The kit features a highly sensitive and specific MPT-NAG method, which ensures accurate detection of NAG levels even at low concentrations. This makes it particularly useful for early detection of renal tubular damage.
2. The composition of the kit includes carefully calibrated reagents and controls, ensuring consistent and reproducible results. The use of human-derived NAG in the calibrator and controls enhances the accuracy of the assay.
3. The primary use of this kit is in clinical laboratories for the diagnosis and monitoring of kidney diseases. It is also used in research settings to study the role of NAG in various pathological conditions.

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) is a vital tool in the clinical assessment of kidney function. Its high sensitivity and specificity make it an indispensable resource for diagnosing renal tubular damage. The kit's well-designed composition and reliable performance ensure accurate and consistent results, making it a trusted choice for healthcare professionals.

Main Features

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) offers high sensitivity, specificity, and reliability in detecting NAG levels in human urine. It is designed for ease of use in clinical laboratories.

1. High Sensitivity: The MPT-NAG method allows for the detection of low concentrations of NAG, making it ideal for early diagnosis of renal tubular damage.
2. Specificity: The kit is highly specific to NAG, reducing the likelihood of cross-reactivity with other enzymes or substances in the urine.
3. Reliability: The inclusion of calibrated reagents and controls ensures consistent and reproducible results across different batches and laboratories.
4. Ease of Use: The kit is designed for straightforward application in clinical settings, with clear instructions and minimal preparation required.
5. Versatility: In addition to clinical diagnostics, the kit can be used in research to study the role of NAG in various diseases and conditions.

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) stands out for its high sensitivity, specificity, and reliability. Its ease of use and versatility make it a valuable tool for both clinical diagnostics and research. The kit's robust design ensures accurate and consistent results, making it a trusted choice for healthcare professionals.

Clinical Applications

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) is primarily used in clinical settings for the diagnosis and monitoring of kidney diseases, particularly those involving renal tubular damage.

1. Diagnosis of Kidney Diseases: The kit is widely used to detect renal tubular damage, which is often an early indicator of kidney disease.
2. Monitoring Disease Progression: By measuring NAG levels over time, healthcare providers can monitor the progression of kidney diseases and adjust treatment plans accordingly.
3. Research Applications: The kit is also used in research to study the role of NAG in various pathological conditions, including diabetes and hypertension.
4. Clinical Trials: The kit is employed in clinical trials to evaluate the efficacy of new treatments for kidney diseases.
5. Routine Screening: In some healthcare settings, the kit is used for routine screening of patients at risk for kidney disease, such as those with diabetes or hypertension.

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) is a versatile tool in clinical practice, used for diagnosing and monitoring kidney diseases. Its application extends to research and clinical trials, making it a valuable resource for advancing our understanding of kidney-related conditions.

Purchasing Guide

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method), consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product Specifications: Review the kit's specifications, including sensitivity, specificity, and shelf life, to ensure it aligns with your laboratory's requirements.
2. Product Types: Determine whether you need a standard kit or one with additional features, such as extended controls or higher sensitivity.
3. Budget: Consider your budget and compare prices from different suppliers to find the best value without compromising on quality.
4. Intended Use: Ensure the kit is suitable for your intended applications, whether for clinical diagnostics, research, or routine screening.

Purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can select a kit that meets your laboratory's needs and ensures accurate and reliable results.

 Precautions

Proper handling and storage of the N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method) are essential to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to prevent degradation of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Use proper laboratory techniques when handling the reagents to avoid contamination. Always wear appropriate personal protective equipment (PPE).
3. Expiration Date: Check the expiration date before use. Do not use expired reagents, as they may yield inaccurate results.
4. Calibration: Regularly calibrate the equipment used with the kit to ensure accurate measurements.
5. Disposal: Dispose of used reagents and materials according to local regulations and laboratory safety protocols.

To ensure the accuracy and reliability of the N-Acetyl-β-D-Glucosaminidase Assay Kit (MPT-NAG Method), it is crucial to follow proper storage, handling, and disposal procedures. Adhering to these precautions will help maintain the integrity of the reagents and ensure consistent results.

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