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N-Acetyl-β-D-Glucosaminidase Assay Kit (MNP-G1CNAc Substrate Method)
The kit consists of Reagent 1 (R1), Reagent 2 (R2), optional quality control, and optional calibrator. Reagent 1 contains 2.5 mmol/L of 2-Methoxy-4-(2'-nitro)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-G1cNAc), 26 mmol/L of citric acid, and 1 mmol/L of disodium ethylenediaminetetraacetate. Reagent 2 contains 175 mmol/L of sodium carbonate buffer. The calibrator and quality control are based on N-Acetyl-β-D-Glucosaminidase.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (MNP-G1CNAc Substrate Method)
Alias:
NAG Assay Kit,Glucosaminidase Test Kit
Intended Use:
This kit is designed for the quantitative determination of N-Acetyl-β-D-Glucosaminidase (NAG) in human urine samples in vitro. It is widely used in clinical diagnostics to assess kidney function and detect renal tubular damage.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit (MNP-G1CNAc Substrate Method) is a highly sensitive and specific diagnostic tool used to measure the activity of NAG in human urine. This enzyme is a key biomarker for renal tubular injury, making the kit essential for early detection and monitoring of kidney diseases. The kit is designed for ease of use, providing accurate and reliable results in clinical laboratories.

1. The kit features a unique substrate, MNP-G1CNAc, which ensures high specificity and sensitivity in detecting NAG activity. This substrate is stable and provides consistent results across various sample types.
2. The composition of the kit includes two main reagents: Reagent 1, which contains the substrate and stabilizing agents, and Reagent 2, which provides the necessary buffer for the enzymatic reaction. The optional calibrator and quality control materials ensure the accuracy and precision of the assay.
3. The primary use of this kit is to quantify NAG levels in urine, which is crucial for diagnosing and monitoring kidney-related conditions. It is particularly useful in detecting early stages of renal tubular damage, allowing for timely intervention and treatment.

The N-Acetyl-β-D-Glucosaminidase Assay Kit, also known as the NAG Assay Kit, is a vital tool in clinical diagnostics. Its unique composition and high sensitivity make it indispensable for assessing kidney health. Whether you're a clinician or a researcher, this kit offers reliable and accurate results, helping you make informed decisions about patient care.

Main Features

The N-Acetyl-β-D-Glucosaminidase Assay Kit offers high sensitivity, specificity, and ease of use, making it a preferred choice for clinical laboratories. It provides accurate quantification of NAG activity, essential for diagnosing kidney diseases.

1. High Sensitivity: The kit uses a specialized substrate, MNP-G1CNAc, which ensures precise detection of NAG activity even at low concentrations.
2. Specificity: The assay is designed to specifically target NAG, minimizing interference from other enzymes or substances in the urine sample.
3. Stability: The reagents are formulated to maintain stability over a wide range of storage conditions, ensuring consistent performance.
4. Ease of Use: The kit comes with clear instructions and requires minimal preparation, making it suitable for routine use in clinical settings.
5. Comprehensive Quality Control: The optional calibrator and quality control materials allow for regular validation of the assay's accuracy and precision.

The N-Acetyl-β-D-Glucosaminidase Assay Kit stands out for its high sensitivity, specificity, and user-friendly design. It's a reliable tool for clinicians and researchers alike, providing accurate results that are crucial for diagnosing and monitoring kidney health.

Clinical Applications

This kit is widely used in clinical settings to assess kidney function and detect renal tubular damage. It is particularly valuable in diagnosing early stages of kidney disease and monitoring treatment efficacy.

1. Early Detection: The kit is highly effective in identifying early signs of renal tubular injury, allowing for prompt intervention.
2. Monitoring Treatment: It is used to monitor the effectiveness of treatments for kidney diseases, providing valuable feedback on patient progress.
3. Research Applications: Beyond clinical use, the kit is also utilized in research studies to explore the role of NAG in various renal conditions.
4. Diagnostic Tool: It serves as a key diagnostic tool in nephrology, helping clinicians make informed decisions about patient care.
5. Quality Assurance: The inclusion of quality control materials ensures that the assay results are reliable and consistent, which is critical in clinical diagnostics.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a versatile tool in both clinical and research settings. Its ability to detect early renal damage and monitor treatment efficacy makes it an invaluable asset in the fight against kidney disease.

Purchasing Guide

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, consider factors such as product specifications, types, budget, and intended use to ensure it meets your laboratory's needs.

1. Product Specifications: Review the kit's components, including reagents, calibrators, and quality control materials, to ensure they align with your laboratory's requirements.
2. Product Types: Choose between different kit formats, such as single-use or bulk options, based on your laboratory's workflow and sample volume.
3. Budget Considerations: Evaluate the cost-effectiveness of the kit, taking into account the number of tests it can perform and the potential savings from bulk purchases.
4. Usage Scenarios: Consider the specific applications of the kit, such as routine diagnostics or research, to ensure it is suitable for your intended use.

Purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit requires careful consideration of your laboratory's needs. By focusing on product specifications, types, budget, and usage scenarios, you can select a kit that delivers reliable and accurate results for your clinical or research applications.

 Precautions

Proper handling and storage of the N-Acetyl-β-D-Glucosaminidase Assay Kit are essential to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to prevent degradation of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling Precautions: Use appropriate personal protective equipment (PPE) when handling the reagents to avoid contamination or exposure.
3. Sample Preparation: Ensure that urine samples are collected and processed according to the kit's instructions to avoid errors in the assay results.
4. Reagent Stability: Check the expiration dates of the reagents and use them within the specified period to ensure optimal performance.
5. Quality Control: Regularly perform quality control tests using the provided materials to verify the accuracy and precision of the assay.

Taking the necessary precautions when using the N-Acetyl-β-D-Glucosaminidase Assay Kit is crucial for obtaining reliable results. Proper storage, handling, and adherence to the kit's instructions will help ensure the success of your diagnostic or research efforts.

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