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N-Acetyl-β-D-Glucosaminidase Assay Kit (CNP-NAG Substrate Method)
The N-Acetyl-β-D-Glucosaminidase Assay Kit is designed for the quantitative determination of N-Acetyl-β-D-Glucosaminidase (NAG) activity in human serum or urine. The kit includes two main reagents: Reagent 1, a Tris buffer with a pH range of 5.0 to 10.0, and Reagent 2, which contains 2-Chloro-4-Nitrophenyl-N-Acetyl-beta-D-Glucosaminide (CNP-NAG) at a concentration of 3.5 mmol/L. These components work together to provide a reliable and accurate measurement of NAG activity, which is crucial for various diagnostic applications.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (CNP-NAG Substrate Method)
Alias:
NAG Assay Kit,CNP-NAG Kit
Intended Use:
This kit is primarily used for the in vitro quantitative determination of N-Acetyl-β-D-Glucosaminidase (NAG) activity in human serum or urine. NAG is an enzyme that plays a significant role in the breakdown of complex carbohydrates, and its activity levels can be indicative of certain medical conditions, such as kidney disease or lysosomal storage disorders. The kit is essential for clinical laboratories and research institutions that require precise and reproducible measurements of NAG activity.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit (CNP-NAG Substrate Method) is a specialized diagnostic tool designed for the accurate measurement of NAG activity in biological samples. This kit is particularly useful in clinical settings where the detection of NAG activity can provide critical insights into various health conditions. The kit includes two key reagents that work in tandem to ensure precise and reliable results, making it an indispensable tool for laboratories focused on metabolic and renal health.

1. The N-Acetyl-β-D-Glucosaminidase Assay Kit is characterized by its high sensitivity and specificity, ensuring accurate detection of NAG activity even at low concentrations. The kit's design allows for easy integration into existing laboratory workflows, making it a practical choice for busy clinical environments.
2. The kit's composition includes two main reagents: Reagent 1, a Tris buffer that maintains the optimal pH for the enzymatic reaction, and Reagent 2, which contains the substrate CNP-NAG. These components are carefully formulated to ensure stability and consistency across different batches, providing reliable results every time.
3. The primary use of this kit is for the quantitative determination of NAG activity in human serum or urine. This measurement is crucial for diagnosing and monitoring conditions such as kidney disease, where elevated NAG levels can indicate renal tubular damage. The kit's accuracy and ease of use make it a valuable tool for clinicians and researchers alike.

The N-Acetyl-β-D-Glucosaminidase Assay Kit, also known as the CNP-NAG Kit, is a highly specialized diagnostic tool that offers precise measurement of NAG activity in biological samples. Its high sensitivity and specificity, combined with its ease of use, make it an essential component of any clinical laboratory focused on metabolic and renal health. The kit's reliable performance and consistent results ensure that clinicians and researchers can trust the data it provides, making it a cornerstone in the diagnosis and monitoring of various health conditions.

Main Features

The N-Acetyl-β-D-Glucosaminidase Assay Kit offers a range of features designed to ensure accurate and reliable measurement of NAG activity. These features include high sensitivity, specificity, and ease of use, making it an ideal choice for clinical laboratories.

1. High Sensitivity: The kit is capable of detecting low levels of NAG activity, ensuring accurate results even in samples with minimal enzyme presence.
2. Specificity: The kit is specifically designed to measure NAG activity, reducing the risk of cross-reactivity with other enzymes and ensuring precise results.
3. Ease of Use: The kit's straightforward protocol allows for easy integration into existing laboratory workflows, minimizing the need for extensive training or specialized equipment.
4. Stability: The reagents are formulated to maintain stability over time, ensuring consistent performance across different batches and storage conditions.
5. Reproducibility: The kit's design ensures that results are reproducible, providing reliable data for clinical decision-making and research purposes.

The N-Acetyl-β-D-Glucosaminidase Assay Kit stands out for its high sensitivity, specificity, and ease of use. These features make it an indispensable tool for clinical laboratories, ensuring accurate and reliable measurement of NAG activity. The kit's stability and reproducibility further enhance its value, making it a trusted choice for clinicians and researchers alike.

Clinical Applications

The N-Acetyl-β-D-Glucosaminidase Assay Kit is widely used in clinical settings for the diagnosis and monitoring of various health conditions, particularly those related to kidney function and lysosomal storage disorders.

1. Kidney Disease Diagnosis: The kit is commonly used to measure NAG activity in urine, which can indicate renal tubular damage and help diagnose kidney disease.
2. Lysosomal Storage Disorders: Elevated NAG levels can also be indicative of lysosomal storage disorders, making the kit a valuable tool for diagnosing these conditions.
3. Monitoring Treatment Efficacy: The kit can be used to monitor the effectiveness of treatments for conditions that affect NAG levels, providing clinicians with critical information for patient management.
4. Research Applications: In addition to clinical use, the kit is also used in research settings to study the role of NAG in various metabolic processes and disease states.
5. Preventive Screening: The kit can be used for preventive screening in populations at risk for kidney disease or lysosomal storage disorders, allowing for early detection and intervention.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a versatile tool with a wide range of clinical applications. Its ability to accurately measure NAG activity makes it invaluable for diagnosing and monitoring kidney disease, lysosomal storage disorders, and other conditions. The kit's use in both clinical and research settings underscores its importance in advancing our understanding of metabolic health and disease.

Purchasing Guide

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use to ensure that the kit meets your laboratory's needs.

1. Product Specifications: Review the kit's specifications, including sensitivity, specificity, and stability, to ensure it meets your laboratory's requirements.
2. Product Types: Consider the different types of kits available, such as those designed for specific sample types or with varying reagent concentrations, to find the best fit for your needs.
3. Budget: Evaluate the cost of the kit in relation to your laboratory's budget, taking into account any potential savings from bulk purchases or long-term contracts.
4. Intended Use: Determine the primary use of the kit, whether for clinical diagnostics, research, or preventive screening, to ensure it aligns with your laboratory's goals and objectives.

Purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit requires careful consideration of various factors, including product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that the kit you choose will meet your laboratory's needs and provide reliable, accurate results for your clinical or research applications.

 Precautions

When using the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to follow proper handling and storage procedures to ensure accurate results and maintain the integrity of the reagents.

1. Storage Conditions: Store the kit at the recommended temperature to maintain the stability and effectiveness of the reagents.
2. Handling: Handle the reagents with care, avoiding contamination and ensuring proper mixing to achieve accurate results.
3. Protocol Adherence: Follow the kit's protocol precisely, including incubation times and reagent volumes, to ensure reliable and reproducible results.
4. Safety Precautions: Wear appropriate personal protective equipment (PPE) when handling the reagents to avoid exposure and ensure safety.
5. Disposal: Dispose of used reagents and samples according to local regulations and laboratory protocols to minimize environmental impact and ensure safety.

Proper handling and storage of the N-Acetyl-β-D-Glucosaminidase Assay Kit are essential for obtaining accurate and reliable results. By adhering to the recommended storage conditions, handling procedures, and safety precautions, you can ensure the integrity of the reagents and the safety of laboratory personnel. Following the kit's protocol and disposal guidelines further enhances the reliability and safety of the assay process.

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