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N-Acetyl-β-D-Glucosaminidase Assay Kit (MNP-GlcNAc Substrate Method)
The N-Acetyl-β-D-Glucosaminidase Assay Kit is designed for the quantitative determination of N-Acetyl-β-D-Glucosaminidase activity in human urine. The kit includes two main reagents: Reagent 1, which contains 2-Methoxy-4(2'-nitro)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside hydrochloride at a concentration of 0.05 mol/L, and Reagent 2, which consists of citric acid (4.5 g/L), potassium phosphate (20 g/L), and a sodium carbonate buffer (pH 9.5) at 50-60 ml/L.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (MNP-GlcNAc Substrate Method)
Alias:
NAG Assay Kit,Glucosaminidase Test Kit
Intended Use:
This kit is specifically used for the in vitro quantitative determination of N-Acetyl-β-D-Glucosaminidase activity in human urine. It is a crucial tool in clinical diagnostics, particularly for assessing renal function and detecting early signs of kidney damage.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a sophisticated diagnostic tool designed to measure the activity of N-Acetyl-β-D-Glucosaminidase in human urine. This enzyme is a key biomarker for renal health, and its activity levels can provide early indications of kidney dysfunction. The kit employs the MNP-GlcNAc substrate method, which is known for its high sensitivity and specificity.

1. The kit features a highly sensitive and specific substrate, 2-Methoxy-4(2'-nitro)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside hydrochloride, which ensures accurate detection of N-Acetyl-β-D-Glucosaminidase activity.
2. The composition of the kit includes two main reagents: Reagent 1, which contains the substrate, and Reagent 2, which provides the necessary buffer system to maintain optimal pH conditions for the enzymatic reaction.
3. The primary use of this kit is in clinical diagnostics, particularly for the early detection of kidney damage. It is also used in research settings to study renal function and related diseases.

The N-Acetyl-β-D-Glucosaminidase Assay Kit, also known as the NAG Assay Kit, is a vital tool in both clinical and research settings. Its high sensitivity and specificity make it an indispensable resource for assessing renal health and detecting early signs of kidney damage.

Main Features

The N-Acetyl-β-D-Glucosaminidase Assay Kit offers high sensitivity and specificity in detecting N-Acetyl-β-D-Glucosaminidase activity, making it a reliable tool for clinical diagnostics and research.

1. The kit utilizes the MNP-GlcNAc substrate method, which is known for its high sensitivity and specificity in detecting N-Acetyl-β-D-Glucosaminidase activity.
2. It includes two main reagents: Reagent 1, which contains the substrate, and Reagent 2, which provides the necessary buffer system for optimal enzymatic reaction conditions.
3. The kit is designed for easy use in clinical laboratories, with a straightforward protocol that ensures accurate and reproducible results.
4. It is particularly useful for the early detection of kidney damage, providing critical information for the diagnosis and management of renal diseases.
5. The kit is also used in research settings to study the role of N-Acetyl-β-D-Glucosaminidase in various physiological and pathological processes.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a highly reliable and sensitive tool for detecting N-Acetyl-β-D-Glucosaminidase activity. Its ease of use and high specificity make it an essential resource for both clinical diagnostics and research.

Clinical Applications

The N-Acetyl-β-D-Glucosaminidase Assay Kit is widely used in clinical settings for the early detection of kidney damage and the assessment of renal function.

1. The kit is used in clinical laboratories to measure N-Acetyl-β-D-Glucosaminidase activity in human urine, providing early indications of kidney damage.
2. It is employed in the diagnosis and management of various renal diseases, including acute kidney injury and chronic kidney disease.
3. The kit is also used in research settings to study the role of N-Acetyl-β-D-Glucosaminidase in renal physiology and pathology.
4. It is particularly useful in monitoring patients with diabetes, as elevated levels of N-Acetyl-β-D-Glucosaminidase activity can indicate diabetic nephropathy.
5. The kit is also used in toxicology studies to assess the nephrotoxic effects of various drugs and chemicals.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a versatile tool in clinical diagnostics and research. Its ability to provide early indications of kidney damage makes it an invaluable resource for the diagnosis and management of renal diseases.

Purchasing Guide

The N-Acetyl-β-D-Glucosaminidase Assay Kit is available for purchase with detailed guidelines on product parameters, types, budget considerations, and usage scenarios.

1. The kit is available in different configurations, including single-use and multi-use formats, to suit various laboratory needs.
2. It is important to consider the specific requirements of your laboratory, such as the volume of samples to be processed, when selecting the appropriate kit type.
3. Budget considerations should include not only the initial purchase cost but also the long-term costs associated with storage, handling, and disposal of the kit components.
4. The kit is suitable for use in a variety of clinical and research settings, including hospitals, diagnostic laboratories, and academic research institutions.

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to consider the specific needs of your laboratory, including the volume of samples to be processed and the long-term costs associated with the kit. The kit is suitable for use in a wide range of clinical and research settings.

 Precautions

Proper handling and storage of the N-Acetyl-β-D-Glucosaminidase Assay Kit are essential to ensure accurate and reliable results.

1. The kit should be stored at the recommended temperature to maintain the stability of the reagents.
2. It is important to follow the manufacturer's instructions for the preparation and use of the reagents to avoid contamination and ensure accurate results.
3. Proper disposal of used reagents and kit components is essential to prevent environmental contamination and ensure laboratory safety.
4. The kit should be used within the specified expiration date to ensure the reliability of the results.
5. It is important to wear appropriate personal protective equipment, such as gloves and lab coats, when handling the kit components to prevent exposure to potentially harmful substances.

The N-Acetyl-β-D-Glucosaminidase Assay Kit requires careful handling and storage to ensure accurate and reliable results. Proper disposal of used reagents and adherence to safety protocols are essential to maintain laboratory safety and environmental health.

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